Course Title: Gene Technologies 1

Part A: Course Overview

Course Title: Gene Technologies 1

Credit Points: 12.00

Course Code




Learning Mode

Teaching Period(s)


Bundoora Campus


135H Applied Sciences


Sem 1 2014,
Sem 1 2015,
Sem 1 2016


Bundoora Campus


171H School of Science


Sem 1 2017

Course Coordinator: Assoc. Professor Aidyn Mouradov

Course Coordinator Phone: +61 3 9925-7144

Course Coordinator Email:

Course Coordinator Location: 223-1- Bundoora West campus

Pre-requisite Courses and Assumed Knowledge and Capabilities

This course assumes knowledge of genetics, cell biology and biochemistry equivalent to the successful completion of a second-year tertiary course .

Course Description

This course aims provide you with an understanding of the principles and methodologies of recently developed gene technologies and the applications of these technologies to the diagnosis and treatment of disease and the development of new products through genetic manipulation. Topic areas include, but may not be limited to, detailed study of molecular biology techniques, particularly gene cloning and expression systems such as plasmids, construction and screening of genomic libraries, foreign gene transfer and expression systems in eukaryotes and prokaryotes, high throughput and next generation DNA sequencing and microarrays and the use of gene expression profiling. How these emerging technologies are used to diagnose disease, develop therapeutics, new foods and transgenic crops will be detailed for you.

Objectives/Learning Outcomes/Capability Development

This course contributes to the following Program Learning Outcomes in MC111 Master of Biotechnology:

 1. Understanding Science 

1.1 You will demonstrate an advanced understanding of biological sciences by articulating the methods of science, explaining why current biological knowledge is both contestable and testable through further inquiry, and explaining the role and relevance of biotechnology in society. 

1.2 You will have an understanding of recent developments in a specialised area of biotechnology 

2. Advanced skills to critically analyse and solve problems in biotechnology. 

2.1 You will demonstrate cognitive skills in the mastery of advanced theoretical knowledge in biotechnology and apply this knowledge to solve complex problems in existing and new areas. 

5. Personal and professional responsibility. 

5.3 You will demonstrate knowledge of the regulatory frameworks and ethical principles relevant to biotechnology


,On the successful completion of this course, you should be able to:

1. Demonstrate theoretical and technical understanding of the molecular structure and function of DNA and the various methods of DNA cloning, strategies, gene expression by prokaryote and eukaryote vectors, detection of gene products and utilisation of cloned products for diagnostic or production purposes.

2. Perform molecular biological procedures safely in the laboratory.

3. Analyse and critically evaluate data.

4. Summarise and critique primary scientific literature.

5. Communicate clearly and effectively using correct scientific language and conventions.




Overview of Learning Activities

You will learn in this course by

participating in structured lectures and practical laboratory classes

your own self-directed learning e.g. computer-assisted learning, reading literature, current publications and internet resources.

Total Study Hours

A total of 120 hours of study is expected during this course, comprising:

Teacher-directed hours (56 hours): Each week there will be 2 hours of face-to-face lecture (24 hours). There will also be 4 full days of laboratory practical sessions (32 hours). You are encouraged to participate during lectures by asking and answering questions and commenting on the lecture material based on your own study.

Student-directed hours (64 hours): You are expected to be self-directed, studying independently outside class. A minimum of 5 hours per week is recommended.



Overview of Learning Resources

The course website provides a syllabus, lecture notes, handouts for laboratory practicals and excursions, sample assignments and test questions, and references to additional resources.

The course is supported by the Blackboard learning management system which provides specific learning resources. See the RMIT Library Guide at


Overview of Assessment

 Note that: This course has no hurdle requirements. 

Assessments comprise:

1. A series of online quizzes (30%)

This assessment supports CLOs 1, 3, 4 & 5 

2. Practical component (20%)

This assessment supports CLOs 2, 3, 4 & 5 

3. Written exam (50%)

This assessment supports CLOs 1, 3, 4 & 5