Course Title: Protein Technologies

Part A: Course Overview

Course Title: Protein Technologies

Credit Points: 12.00


Course Code

Campus

Career

School

Learning Mode

Teaching Period(s)

BIOL2226

City Campus

Postgraduate

135H Applied Sciences

Face-to-Face

Sem 2 2006,
Sem 2 2007,
Sem 2 2009,
Sem 2 2011,
Sem 2 2012,
Sem 2 2015,
Sem 2 2016

Course Coordinator: A/Prof Ian Macreadie

Course Coordinator Phone: +613-9925 6627

Course Coordinator Email: ian.macreadie@rmit.edu.au

Course Coordinator Location: Bundoora West Campus, Bld. 223, level 1, R. 28C

Course Coordinator Availability: By appointment before or after the class


Pre-requisite Courses and Assumed Knowledge and Capabilities

Satisfactory completion of at least a second year course in biochemistry (such as BIOL2260 or equivalent).


Course Description

The course will present the theory and techniques of modern protein chemistry and proteomics and their application in the study of protein structure and function and their interactions.

On successful completion of this course, you will have acquired:

  1. advanced knowledge of the underlying principles of protein technologies and its application in solving problems in biological
  2. some practical skills for the isolation, identification and characterisation of proteins
  3. an understanding of how a range of analytical, synthetic and molecular biology methodologies can be combined to elucidate structure-function relationships in proteins
  4. practical experience with the fundamental techniques of proteomics and knowledge of high-throughput automated strategies used in profiling of proteomes
  5. the ability to mine, track, filter and interpret complex data sets generated from proteomic analyses
  6. an awareness of current research activities and technology developments in this field
  7. skills in information retrieval, critical review and analysis of scientific data and its presentation in a range of scientific data and its presentation in a range of formats.


Objectives/Learning Outcomes/Capability Development

This course contributes to the following Program Learning Outcomes at AQF Level 9:

Understanding Science Demonstration of advanced understanding and recent developments in biotechnology sciences


At the conclusion of the course you should be able to:

  1. Describe the properties of a protein through analysis of its amino acid sequence;
  2. Purify proteins through a range of approaches;
  3. Use bioinformatics to analyse proteomes;
  4. Produce proteins using a range of approaches, including synthetic chemistry and biological methods;
  5. Retrieve and analyse biological data that will assist laboratory work
  6. Develop and utilise Protein Technologies to solve problems


Overview of Learning Activities

A series of lectures by both research and industrial experts in protein technologies will be presented which will be supported by some practical exercises and a workshop on chromatography.

Total study hours

Three hours each week are used for teacher guided activities, including lectures from experts in Protein Technologies as well as Practical activities and tutorials.  One week includes a wet lab.  In addition you are expected to spend a minimum of four hours per week on independent study.


Overview of Learning Resources

A range of learning resources will be used including web-based resources, textbooks, journal articles, lecture notes and training with relevant instrumentation including mass spectrometry.


Overview of Assessment

All hurdle requirements for this course are indicated clearly in the assessment regime that follows, against the relevant assessment task(s) and all have been approved by the College Deputy Pro Vice-Chancellor (Learning & Teaching).

The course will be assessed by individual or group projects and by theory examination (50%), on-line test (10%), practicals (20%) and a critical analysis (of a Protein Technology) (20%).  Each assessment supports the following CLOs:

  1. theory examination = CLO #1, 2, 3, 4 and 6
  2. on-line test = CLO #1, 2, 3, 4
  3. Practicals (HURDLE) = CLO #1, 2, 3, 4 and 5
  4. critical analysis = CLO #1, 2, 3, 4, 5 and 6

The Prac assessments are a hurdle for this course, meaning that you must receive more than 50% on the Practical component in order to pass the course. The skills learned in Practical are essential to ensure that you have practical experience in laboratory safety and competency, as well as skills in handling basic and more complex data obtained protein technologies. This hurdle has been approved by the SEH PVC Learning & Teaching.