Course Title: Cell and Tissue Culture

Part A: Course Overview

Course Title: Cell and Tissue Culture

Credit Points: 12.00


Terms

Course Code

Campus

Career

School

Learning Mode

Teaching Period(s)

BIOL2264

City Campus

Undergraduate

135H Applied Sciences

Face-to-Face

Sem 1 2016

BIOL2264

City Campus

Undergraduate

171H School of Science

Face-to-Face

Sem 1 2017

Course Coordinator: Dr. Ravi Shukla

Course Coordinator Phone: +61 3 9925 2970

Course Coordinator Email: ravi.shukla@rmit.edu.au

Course Coordinator Location: 3.3.11

Course Coordinator Availability: Email for appointment


Pre-requisite Courses and Assumed Knowledge and Capabilities

Assumed Knowledge and Capabilities:

Knowledge of and practical skills in plant and animal anatomy and physiology, as obtained for example in BIOL2156 Plant Structure and Function, BIOL2258 Animal Structure & Function;

and/or

Knowledge of and practical skills of cloning, microbial contamination and sterile techniques as obtained in BIOL2256/2257 Introduction to Microbiology, Immunology and Genetics,

and/or

An equivalent course or evidence of equivalent capabilities.


Course Description

This course provides knowledge of and expertise in animal and plant tissue culture theory and practice. This course has a vocational focus and introduces you to the theory and practice of animal and plant tissue culture with their role and applications in biotechnology and biochemical research.

The topics covered in this course include media preparation, sterile techniques, aseptic handling, initiation and routine maintenance of cells in culture, common contaminants of plant and animal cell culture, and understanding of some of the applications of cell culture technology e.g. somatic cell and protoplast fusion; Hybridoma technology.


Objectives/Learning Outcomes/Capability Development

This course contributes to the following Program Learning Outcomes in BP229 Bachelor of Science:

 

Understanding Science.

1.1 You will be able to articulate the methods of science and explain why current scientific knowledge is contestable and testable through further inquiry.

Scientific knowledge:

2.1. You will demonstrate a depth and breadth of knowledge and understanding of biological sciences.

Inquiry and problem solving:

3.4. You will be able to analyse and solve problems in biotechnology by collecting, accurately recording, interpreting, and drawing conclusions from scientific data.

Personal and professional responsibility.

5.2 You will work responsibly, safely, legally and ethically in an individual and team context.


On successful completion of this course you should be able to:

  1. Explain major components of cell and tissue culture media, e.g. minerals, growth factors, hormones, and what governs the choice of components.
  2. Explain steps taken to establish and optimise media for different species and cell lines, without the aid of texts.
  3. Perform the common cell culture techniques, e.g. callus culture, Embryo culture and embryogenesis in plants, culture of animal cells.
  4. Demonstrate knowledge of cell lines used in mammalian tissue culture, their origins and applications.
  5. Competently perform laboratory procedures and demonstrate practical application and conceptual knowledge of cell and plant tissue culture for biotechnology investigations and applications.


Overview of Learning Activities

The learning activities included in this course are:

  1. your attendance at lectures where syllabus material will be presented and explained, and the subject will be illustrated with demonstrations and examples;
  2. answering the questions designed to give feedback on your progress and understanding;
  3. completion of practical work and assignments consisting of numerical and other problems requiring an integrated understanding of the subject matter; and
  4. private study, working through the course as presented in classes and learning materials, and gaining practice at solving conceptual problems.

Total study hours:

A total of 120 hours comprises

Teacher-guided learning (54 hours):

  • 2 hours of lectures per week (24 hours)
  • 2 hours 30 mins of laboratory practical class per week (30 hours; and

Self-directed learning (66 hours):

  • A minimum of 5 and a half hours per week of independent study.


Overview of Learning Resources

You will access course information and learning material through the myRMIT where the course web site provides a syllabus, lecture notes, handouts for laboratory practicals, assignments and test questions, and references to additional resources. You will be provided with the lists of relevant texts, library resources (including appropriate journals) and freely accessible Internet sites. Other material will be provided in class.


Overview of Assessment

Note that:

The skills learned in Practical classes are essential to ensure that you can operate safely as a professional in laboratory settings.

 

A: The practical assessment (35%):

1. Laboratory tasks (25%)

Assessed on written answer sheets and practical reports completed from observations of biological specimens. You must attend at least 75 % of laboratory sessions and complete all laboratory assessment tasks in order to pass this assessment.

This assessment supports CLOs 1-5

2. Practical performance (10%)

You will be required to demonstrate competence in laboratory skills as assessed by the demonstrator during classes.

This assessment supports CLOs 3 and 5

B: The Online assessment (25%)

1.Three open- book online quizzes

This assessment supports CLOs 1, 2, 4 and 5

2. Written assignment to assess your critical thinking ability

This assessment supports CLOs 3 and 5

 

C: End-of-semester examination (40%)

This assessment supports CLOs 1-5