Course Title: Cell and Tissue Culture

Part A: Course Overview

Course Title: Cell and Tissue Culture

Credit Points: 12.00

Course Code




Learning Mode

Teaching Period(s)


City Campus


135H Applied Sciences


Sem 1 2016


City Campus


171H School of Science


Sem 1 2017

Course Coordinator: Dr. Ravi Shukla

Course Coordinator Phone: +61 3 9925 2970

Course Coordinator Email:

Course Coordinator Location: 223.1.01

Course Coordinator Availability: Email for appointment

Pre-requisite Courses and Assumed Knowledge and Capabilities

Knowledge of and practical skills in plant and animal anatomy and physiology, as obtained for example in BIOL2156 Plant Structure and Function, BIOL 2258/9 Animal Structure & Function and of cloning, microbial contamination and sterile techniques as obtained in BIOL 2256/2257 Introduction to Microbiology, Immunology and Genetics, an equivalent course or evidence of equivalent capabilities.

Course Description

This course provides graduate-level knowledge of and expertise in animal and plant tissue culture theory and practice.
This course has a vocational focus and introduces the you to the theory and practice of animal and plant tissue culture and their role from modifying plants in plant biotechnology to the propagation of endangered plants and from modifying cell lines in biotechnology to the propagation of all lines for use in medical, microbiological and biochemical research. It prepares you in particular for a career with plants, both in plant biotechnology and in environmental biotechnology. You study media, sterilisation, explants, micropropagation, callus culture, organogenesis, embryogenesis, somatic variation, doubled haploids, interspecific hybrids, protoplast fusion and environmental conditions required. These are related to uses of cell and tissue culture and compared with traditional techniques.

Objectives/Learning Outcomes/Capability Development

This course contributes to the following Program Learning Outcomes (PLOs):

2.1. You will exhibit depth and breadth of knowledge by demonstrating a well-developed understanding of biological sciences.

3.4. You will be able to critically analyse and solve problems in biotechnology by collecting, accurately recording, interpreting, and drawing conclusions from scientific data.

By the end of the course, you should be able to:
• Explain the various components of cell and tissue culture media, e.g. minerals, growth factors, hormones, and what governs the choice of components
• Explain the various steps taken to establish and optimise media for particular purposes in different species and cell lines, without the aid of texts
• Explain and perform the common cell culture techniques, e.g. callus culture, micropropagation, embryogenesis in plants, cell culture in mammalian cells
• Explain the various cell lines used in mammalian tissue culture and their origins and uses


At the conclusion of this course you will understand how to initiate, grow and harvest eukaryotic cells and their uses.

Overview of Learning Activities

The learning activities included in this course are:
• attendance at lectures where syllabus material will be presented and explained, and the subject will be illustrated with demonstrations and examples;
• completion of questions designed to give feedback on student progress and understanding;
• completion of practical work designed to give practice in the application of theory and procedures;
• completion of written assignments consisting of numerical and other problems requiring an integrated understanding of the subject matter; and
• private study, working through the course as presented in classes and learning materials, and gaining practice at solving conceptual and numerical problems.

Overview of Learning Resources

You will be provided with lists of relevant texts, library resources (Including appropriate journals) and freely accessible Internet sites. Other material will be provided in class. 

You will be able to access course information and learning material through the Learning Hub (also known as online@RMIT) and or through CD-ROM.


Overview of Assessment

Learning will be assessed by an end of semester examination, tests and practical reports.