Course Title: Applied Biochemical Methods

Part A: Course Overview

Course Title: Applied Biochemical Methods

Credit Points: 12.00

Terms

Course Code

Campus

Career

School

Learning Mode

Teaching Period(s)

BIOL2301

Bundoora Campus

Undergraduate

160H Medical Sciences

Face-to-Face

Sem 1 2008,
Sem 1 2009,
Sem 1 2010,
Sem 1 2011,
Sem 1 2012,
Sem 1 2013,
Sem 1 2014,
Sem 1 2015,
Sem 1 2016

BIOL2301

Bundoora Campus

Undergraduate

173H School of Health and Biomed

Face-to-Face

Sem 1 2017,
Sem 1 2018

BIOL2322

Bundoora Campus

Postgraduate

160H Medical Sciences

Face-to-Face

Sem 1 2006,
Sem 1 2008,
Sem 1 2009,
Sem 1 2010,
Sem 1 2011,
Sem 1 2012,
Sem 1 2013,
Sem 1 2014,
Sem 1 2015,
Sem 1 2016

BIOL2322

Bundoora Campus

Postgraduate

173H School of Health and Biomed

Face-to-Face

Sem 1 2017,
Sem 1 2018

Course Coordinator: Assoc Prof Terrence Piva

Course Coordinator Phone: +61 3 9925 6503

Course Coordinator Email: terry.piva@rmit.edu.au

Course Coordinator Location: 201.09.06


Pre-requisite Courses and Assumed Knowledge and Capabilities

System Enforced Pre-requisites (Enforced by SAMS)

None.

Assumed Knowledge

 


Course Description

This course will examine modern methods and technologies that are used in biochemical analysis with emphasis on instrumentation, underlying principles, aims, strategies and current applications.  The instrumental techniques covered include the separation and purification of molecules from biological samples, functional genomic assays, mass spectrometry, proteomics, chromatography (including gas chromatography and high-performance liquid chromatography), electrophoresis, as well as an introduction to bioinformatics and enzyme kinetics.  Advances in biochemical research have largely depended on parallel advances in the technology of measuring and observing devices. Knowledge of these devices, new instrumentation methods and the basic principles behind analytical and industrial procedures will assist in general understanding of the equipment selection and an appreciation of good quality assurance and research procedures.

The theory learned during the semester will be applied in practical classes where these techniques will be used for the analysis of biochemical samples in a laboratory setting.  The combination of learning activities offered in this course is designed to equip graduates with a range of attributes that will give the student strong potential and ability to work equally well in analytical or research laboratories.


Objectives/Learning Outcomes/Capability Development

This Biomedical Science program learning outcomes for this course are:

  • PLO 2: Exhibit a breadth and depth of scientific knowledge;
  • PLO 3: Critically analyse and solve scientific problems;
  • PLO 5: Demonstrate accountability for your own learning and scientific work


On the successful completion of this course, you should be able to:

  1. Evaluate the methods and techniques used in the separation, purification, analysis and quantification of molecules (nucleic acids and proteins) in biological samples
  2. Understand the basic principles that underpin Bioinformatics analyses, and develop the ability to apply these when analysing biological data
  3. Interpret and analyse the biological activity of enzymes
  4. Be able to use different instrumentation in the detection and analysis of biological material in a laboratory setting
  5. Analyse and evaluate experimentally derived data and present those results in the form of a rigorous scientific report.


Overview of Learning Activities

The lectures will provide an overview of how technologies are used to detect and quantify a wide range of molecules in biological samples. The laboratory component will emphasize the importance of these technologies in the analysis of biological samples from both an analytical or research context.

The course consists of two hours of lectures scheduled per week for 11 weeks, as well as four hours per week of laboratory work for 7 weeks, and a two hour workshop for 3 weeks during this course. In addition to the face-to-face component of the course, you can expect to spend a minimum of five hours per week in independent study.


Overview of Learning Resources

Learning resources used in this course include text books, lecture notes (PowerPoint presentations), Echo360 recordings of lecture presentations, practical exercises, self-study questions, workshops, and additional references on selected topics. The course site in myRMIT provides opportunities for you to independently access much of this material and use asynchronous discussions as an additional mode of learning.


Overview of Assessment

This course has no hurdle requirements.

Assessment Tasks

Practical reports

Weighting 25%

This assessment task supports CLOs 1, 3, 4 & 5

Mid-semester test

Weighting 15%

This assessment task supports CLO 1

Assignment

Weighting 10%

This assessment task supports CLOs 1 & 2

Online quizzes

Weighting 10%

This assessment task supports CLOs 1, 2 & 3

End of semester exam

Weighting 40%

This assessment task supports CLOs 1, 2 & 3