Course Title: Perform microbiological tests
Part B: Course Detail
Teaching Period: Term1 2008
Course Code: ONPS5203C
Course Title: Perform microbiological tests
School: 155T Vocational Health and Sciences
Campus: City Campus
Program: C5182 - Diploma of Laboratory Technology (Pathology Testing)
Course Contact: Dinah van Ruyven
Course Contact Phone: (03) 9925 4287
Course Contact Email: dinah.vanruyven@rmit.edu.au
Name and Contact Details of All Other Relevant Staff
Barbara Porter
Nominal Hours: 140
Regardless of the mode of delivery, represent a guide to the relative teaching time and student effort required to successfully achieve a particular competency/module. This may include not only scheduled classes or workplace visits but also the amount of effort required to undertake, evaluate and complete all assessment requirements, including any non-classroom activities.
Pre-requisites and Co-requisites
PMLTEST407A Perform biological procedures
Course Description
This unit of competency describes the ability of technical personnel to contribute to the culture, isolation and identification of micro-organisms for investigating the physiology and pathology of plants and animals; for monitoring the natural environment; and to assist in the production of foods, pharmaceutical goods and other manufactured materials.
This unit of competency provides for the development of skill in procedures that can be applied in investigations of bacteria, fungi, viruses, protozoans, algae and parasites, as well
as addressing the broader needs of biotechnology and tissue culture applications.
National Codes, Titles, Elements and Performance Criteria
National Element Code & Title: |
PMLTEST501B Perform microbiological tests |
Element: |
Contribute to antibiotic sensitivity testing where required |
Performance Criteria: |
practical activities |
Element: |
Estimate the number and/or size of micro-organisms in samples |
Performance Criteria: |
practical activities |
Element: |
Maintain records of laboratory work |
Performance Criteria: |
logbook, prac reports |
Element: |
Prepare for safe microbiological work and aseptic applications |
Performance Criteria: |
practical activities, prac exam |
Element: |
Prepare samples to concentrate material for subsequent staining or microscopy |
Performance Criteria: |
practical activities |
Element: |
Process samples for direct examination |
Performance Criteria: |
practical activities |
Element: |
Receive samples and process associated request forms |
Performance Criteria: |
practical activities |
Element: |
Sub culture on suitable media to optimise production of pure cultures |
Performance Criteria: |
practical activities, prac exam |
Learning Outcomes
Details of Learning Activities
Practical exercises, lectures, discussions, class activities, group work and peer teaching, research activities
Teaching Schedule
Week Date Topic
1 12/2 Introduction, Eukaryotic cells
2 19/2 Aseptic technique, safety in the lab
3 26/2 smears
4 4/3 Bacterial growth, requirements and measurement
5 11/3 Bacterial metabolism and incubation conditions
6 18/3 Controlling microbial growth
25/3 Easter break
7 31/3 Mid semester exams
8 7/4 Culture media
9 14/4 Microbial genetics
10 21/4 Classification
11 28/4 Colony morphology
12 5/5 Identification tests
13 12/5 Identification tests
14 19/5 Gram positive bacteria
15 26/5 Gram negative bacteria
16 2/6 revision
17 9/6 Exam
Semester 2
Week Date
1 7/7 Normal human flora, body’s defence mechanisms
2 14/7 epidemiology
3 21/7 antimicrobials
4 29/7 Sample collection and transport
5 4/8 virology
6 11/8 Skin & wound infections
7 18/8 UTI’s
8 25/8 exams
9 1/9 Genital infections
10 8/9 Respiratory infections
11 15/9 CNS, blood infections
22/9 break
12 29/9 Gastro infections
13 6/10 Gastro parasites
14 13/10 Other parasites
15 20/10 revision
16 27/10 Final exams
Learning Resources
Prescribed Texts
Tortora, Funke & Case. 2007,Microbiology: An Introduction, 9th ed. : Pearson Benjamin Cummings U.S. |
References
Other Resources
Overview of Assessment
Assessments for this course typically consist of:
• Class activities
• Assignments
• Presentations
• Exam
Assessment Tasks
2 x Unit Tests per semester: @ 30 % each 60%
3 x Formal Practical Reports per semester: @ 5 % each 15%
1 x Practical Test and observation per semester 25%
Log Book C/NYC
Assessment Matrix
Course Overview: Access Course Overview