Course Title: Perform microbiological tests

Part B: Course Detail

Teaching Period: Term1 2008

Course Code: ONPS5203C

Course Title: Perform microbiological tests

School: 155T Vocational Health and Sciences

Campus: City Campus

Program: C5182 - Diploma of Laboratory Technology (Pathology Testing)

Course Contact: Dinah van Ruyven

Course Contact Phone: (03) 9925 4287

Course Contact Email: dinah.vanruyven@rmit.edu.au


Name and Contact Details of All Other Relevant Staff

Barbara   Porter

Nominal Hours: 140

Regardless of the mode of delivery, represent a guide to the relative teaching time and student effort required to successfully achieve a particular competency/module. This may include not only scheduled classes or workplace visits but also the amount of effort required to undertake, evaluate and complete all assessment requirements, including any non-classroom activities.

Pre-requisites and Co-requisites

PMLTEST407A Perform biological procedures

Course Description

This unit of competency describes the ability of technical personnel to contribute to the culture, isolation and identification of micro-organisms for investigating the physiology and pathology of plants and animals; for monitoring the natural environment; and to assist in the production of foods, pharmaceutical goods and other manufactured materials.
This unit of competency provides for the development of skill in procedures that can be applied in investigations of bacteria, fungi, viruses, protozoans, algae and parasites, as well
as addressing the broader needs of biotechnology and tissue culture applications.


National Codes, Titles, Elements and Performance Criteria

National Element Code & Title:

PMLTEST501B Perform microbiological tests

Element:

Contribute to antibiotic sensitivity testing where required

Performance Criteria:

practical activities

Element:

Estimate the number and/or size of micro-organisms in samples

Performance Criteria:

practical activities

Element:

Maintain records of laboratory work

Performance Criteria:

logbook, prac reports

Element:

Prepare for safe microbiological work and aseptic applications

Performance Criteria:

practical activities, prac exam

Element:

Prepare samples to concentrate material for subsequent staining or microscopy

Performance Criteria:

practical activities

Element:

Process samples for direct examination

Performance Criteria:

practical activities

Element:

Receive samples and process associated request forms

Performance Criteria:

practical activities

Element:

Sub culture on suitable media to optimise production of pure cultures

Performance Criteria:

practical activities, prac exam


Learning Outcomes



Details of Learning Activities

Practical exercises, lectures, discussions, class activities, group work and peer teaching, research activities


Teaching Schedule

Week Date Topic
1 12/2 Introduction, Eukaryotic cells
2 19/2 Aseptic technique, safety in the lab
3 26/2 smears
4 4/3 Bacterial growth, requirements and measurement
5 11/3 Bacterial metabolism and incubation conditions
6 18/3 Controlling microbial growth
25/3 Easter break
7 31/3 Mid semester exams
8 7/4 Culture media
9 14/4 Microbial genetics
10 21/4 Classification
11 28/4 Colony morphology
12 5/5 Identification tests
13 12/5 Identification tests
14 19/5 Gram positive bacteria
15 26/5 Gram negative bacteria
16 2/6 revision
17 9/6 Exam
Semester 2
Week Date
1 7/7 Normal human flora, body’s defence mechanisms
2 14/7 epidemiology
3 21/7 antimicrobials
4 29/7 Sample collection and transport
5 4/8 virology
6 11/8 Skin & wound infections
7 18/8 UTI’s
8 25/8 exams
9 1/9 Genital infections
10 8/9 Respiratory infections
11 15/9 CNS, blood infections
22/9 break
12 29/9 Gastro infections
13 6/10 Gastro parasites
14 13/10 Other parasites
15 20/10 revision
16 27/10 Final exams


Learning Resources

Prescribed Texts

Tortora, Funke & Case. 2007,Microbiology: An Introduction, 9th ed. : Pearson Benjamin Cummings U.S.


References


Other Resources


Overview of Assessment

Assessments for this course typically consist of:
• Class activities
• Assignments
• Presentations
• Exam


Assessment Tasks

2 x Unit Tests per semester: @ 30 % each 60%
3 x Formal Practical Reports per semester: @ 5 % each 15%
1 x Practical Test and observation per semester 25%
Log Book C/NYC


Assessment Matrix

Course Overview: Access Course Overview