Course Title: Perform aseptic techniques
Part B: Course Detail
Teaching Period: Term2 2009
Course Code: ONPS5197C
Course Title: Perform aseptic techniques
School: 155T Vocational Health and Sciences
Campus: City Campus
Program: C5183 - Diploma of Laboratory Technology (Biotechnology)
Course Contact: Gwen Macdonald
Course Contact Phone: +61 3 9925 4283
Course Contact Email: gwen.macdonald@rmit.edu.au
Name and Contact Details of All Other Relevant Staff
Nominal Hours: 40
Regardless of the mode of delivery, represent a guide to the relative teaching time and student effort required to successfully achieve a particular competency/module. This may include not only scheduled classes or workplace visits but also the amount of effort required to undertake, evaluate and complete all assessment requirements, including any non-classroom activities.
Pre-requisites and Co-requisites
Nil
Course Description
This unit of competency covers the ability to perform aseptic techniques to maintain the integrity of both the sample source and the sample. It applies to sampling techniques in tissue culture and to generic microbiological procedures.
National Codes, Titles, Elements and Performance Criteria
National Element Code & Title: |
PMLTEST305B Perform aseptic techniques |
Element: |
Maintain work area and equipment to prevent cross-infection and contamination |
Performance Criteria: |
Serilises equipment as required to prevent cross contamination of work area, personnel and environment |
Element: |
Prepare for aseptic sampling or transfer |
Performance Criteria: |
Prevents cross contamination of source and sample |
Element: |
Transfer materials aseptically |
Performance Criteria: |
Manipulates equipment to prevent contamination of culture medium during transfer |
Learning Outcomes
Details of Learning Activities
Classroom teaching
Laboratory sessions
Teaching Schedule
| Asepsis – what is it? Bacteria intro OHS |
13.7.09 |
| Biohazard cabinet theory and use. Correct technique for transfer Media intro |
20.7.09 |
| Autoclave theory | 27.07.09 |
| Media theory | 3.8.09 |
| Disinfection and sterility theory | 11.8.09 |
| Assess technique-self assessment Prion, fungi, viral theory |
18.8.09 |
| Prac assessment | 24.8.09 |
| Theory test | 31.08.09 |
| Clinical bacterial culture- single colony culture theory G stain theory Tests for bacterial ID- single colony Clinical applications |
7.9.09 |
| Contamination causes and effects- food, wounds etc. | 14.9.09 |
| Transport media, special media unusual organisms | 21.9.09 |
| Antibiotics | 5.10.09 |
| Bacteriocidal agents,antisepsis theory, cross contamination | 12.10.09 |
| Review/Test | 19.10.09 /26.9.09
|
Learning Resources
Prescribed Texts
References
Other Resources
Overview of Assessment
Assessments for this course typically consist of:
• Class activities
• Assignments
• Presentations
• Exam
Assessment Tasks
Theory tests (2) 30% each at end of each term
Practical reports (2) 20%
Assignment on disinfection/autoclave 20%
Demonstrate competency in aseptic transfer giving no contamination of products
Demonstrate competency in observation of OHS guidelines
Assessment Matrix
| Prepare for aseptic transfer | Observation in practical classes and tests |
Basic bacteriology theory Theory of disinfection, sterilisation |
| Transfer material aseptically |
Self assessment of product of aseptic transfer Production of sterile plates and non contaminated media during class |
Theory of biohazard cabinet and autoclave use Single bacterialcolony production theory. |
| Maintain work area and equipment to prevent contamination |
Production of non contaminated work OHS guidelines followed |
Reasons for contamination of media and plates
|
Course Overview: Access Course Overview