Course Title: Perform tissue and cell culture techniques
Part B: Course Detail
Teaching Period: Term2 2010
Course Code: ONPS5209C
Course Title: Perform tissue and cell culture techniques
School: 155T Vocational Health and Sciences
Campus: City Campus
Program: C5183 - Diploma of Laboratory Technology (Biotechnology)
Course Contact: Dinah van Ruyven
Course Contact Phone: +61 3 9925 4287
Course Contact Email: dinah.vanruyven@rmit.edu.au
Name and Contact Details of All Other Relevant Staff
Nominal Hours: 100
Regardless of the mode of delivery, represent a guide to the relative teaching time and student effort required to successfully achieve a particular competency/module. This may include not only scheduled classes or workplace visits but also the amount of effort required to undertake, evaluate and complete all assessment requirements, including any non-classroom activities.
Pre-requisites and Co-requisites
PMLTEST407A - Perform biological procedures
PMLTEST412A - Prepare tissue and cell cultures.
Course Description
This unit of competency covers the ability to prepare, maintain and preserve cells and cell lines for a variety of applications, such as large scale culture, production of monoclonal antibodies, production of viral vaccines and amniocentesis studies. Personnel are required
to optimise equipment set-up, media and growth techniques. They are required to detect and investigate contamination and take preventative and/or corrective actions under supervision.
National Codes, Titles, Elements and Performance Criteria
National Element Code & Title: |
PMLTEST517A Perform tissue and cell culture techniques |
Element: |
Assemble and maintain tissue culture equipment |
Performance Criteria: |
3.1 Assemble, sterilise or decontaminate equipment according to enterprise procedures |
Element: |
Interpret and schedule production requirements |
Performance Criteria: |
|
Element: |
Maintain records |
Performance Criteria: |
7.1 Maintain records of batches of media and test data |
Element: |
Obtain, monitor and maintain tissue and cell lines |
Performance Criteria: |
5.1 Retrieve/obtain the cell lines or tissue sample from fresh or preserved sources and prepare a culture |
Element: |
Prepare and test cell and tissue culture media |
Performance Criteria: |
4.1 Confirm media specifications and processes/methods |
Element: |
Preserve cells and tissues |
Performance Criteria: |
6.1 Select the appropriate preservation method |
Element: |
Work safely according to the legal and regulatory framework |
Performance Criteria: |
2.1 Ensure work practices and personal actions conform to regulations, codes, guidelines and enterprise quality assurance procedures |
Learning Outcomes
Details of Learning Activities
Teacher directed delivery of lessons
Whole class discussion
Pair/group discussion and self assessment
Worksheets
Laboratory experiments
Record keeping of experiments
Research activities
Teaching Schedule
Week 1 | 13.07 | Introduction | Cress practical |
Week 2 | 20.07 | Tissue Culture Environment | Practical two: Media Preparation |
Week 3 | 27.07 | Culture medium and Contamination | Practical two: plant preparation |
Week 4 | 03.08 | Micropropagation | Arapidopsis practical |
Week 5 | 10.08 | Cell culture types and transgenic plants | Protoplast production |
Week 6 | 17.08 | NO class | Arabidopsis practical due |
Week 7 | 24.08 | Revision | Stem cell talk on Monday 23.08 |
Week 8 | 31.08 | Exams | |
Week 9 | 07.09 | Plant tissue culture assessment | |
Week 10 | 14.09 | Plant tissue culture assessment | |
Week 11 | 21.09 | Vacation | |
Week 12 | 28.09 | No class | Journal article |
Week 13 | 05.10 | In vitro fertilization | |
Week 14 | 12.10 | Hybridoma technology | |
Week 15 | 19.10 | Talks | ESSAY due |
Week 16 | 26.10 | Talks | online revision |
Week 17 | 02.11 | exams | |
Week 18 | 09.11 | Additional assessment | |
Learning Resources
Prescribed Texts
References
Other Resources
There is no formal text however students will be referred to several key texts and websites during the course
Overview of Assessment
Assessments for this course typically consist of:
• Class activities
• Assignments
• Presentations
• Exam
Assessment Tasks
Details will be provided in class
Assessment Matrix
Other Information
Outdoor clothing and bags must be left in the areas provided
Laboratory coats must be worn in the laboratory at all times
Long hair must be tied back
Suitable covered footwear must be worn in the laboratory at all times
Correct gloves for the task should be worn at any time when handling substances
Dispose of specialized wastes in containers reserved for the particular type of waste
All accidents and incidents must be reported to the demonstrator
At the end of the session, return all apparatus and reagents to the appropriate places
Ensure that all materials to be disposed of are done according to instructions
Before leaving the laboratory always wash your hands with soap and water
There is to be no eating, drinking or speaking on mobile phones during class
Course Overview: Access Course Overview