Course Title: Perform microscopic examination

Part B: Course Detail

Teaching Period: Term1 2011

Course Code: ONPS5656C

Course Title: Perform microscopic examination

School: 155T Vocational Health and Sciences

Campus: City Campus

Program: C5282 - Diploma of Laboratory Technology (Biotechnology)

Course Contact: Amberlee Mitton

Course Contact Phone: +61 3 9925 8053

Course Contact Email:

Name and Contact Details of All Other Relevant Staff

Gwen MacDonald

Nominal Hours: 40

Regardless of the mode of delivery, represent a guide to the relative teaching time and student effort required to successfully achieve a particular competency/module. This may include not only scheduled classes or workplace visits but also the amount of effort required to undertake, evaluate and complete all assessment requirements, including any non-classroom activities.

Pre-requisites and Co-requisites

there are no pre-requisites or co-requisites for this course


Course Description

This unit of competency covers the ability to set up a light microscope for optimum resolution, to prepare routine samples and to observe, identify and report sample characteristics.


National Codes, Titles, Elements and Performance Criteria

National Element Code & Title:

MSL973007A Perform microscopic examination


1. Interpret test requirements

Performance Criteria:

1.1 Review test request to identify samples to be tested, test method and equipment involved

1.2 Identify hazards associated with the sample, preparation methods and equipment and implement enterprise control measures


2. Set up work area for preparation and examination of samples

Performance Criteria:

2.1 Collect equipment and arrange the workspace so that equipment can be used safely and efficiently

2.2 Perform pre-use and safety checks to ensure equipment is fit for purpose and report faulty or unsafe equipment to appropriate personnel


3. Prepare samples for examination

Performance Criteria:

3.1 Log and label samples according to enterprise procedures to ensure traceability

3.2 Check suitability of the original and prepared sample for the examination and report unsuitable samples to appropriate personnel

3.3 Prepare and store the sample for examination following enterprise methods


4. Set up and use a light microscope

Performance Criteria:

4.1 Set up the light path to optimise resolution

4.2 Select the appropriate objectives and filter for the sample being examined

4.3 Ensure that the lenses are clean

4.4 Adjust settings and alignment of the light path to optimise performance

4.5 Place sample correctly on the stage


5. Observe, identify and report sample characteristics

Performance Criteria:

5.1 Recognise and identify significant sample characteristics

5.2 Perform required calculations accurately

5.3 Prepare and view control samples and check that results are consistent with expected values

5.4 Identify and report out of specification or atypical results promptly to appropriate personnel

5.5 Record and report data in accordance with enterprise procedures


6. Maintain a safe work environment

Performance Criteria:

6.1 Ensure safety and minimise cross-contamination through the use of personal protective clothing and safety equipment

6.2 Handle all samples and equipment in accordance with enterprise safety protocols

6.3 Clean up spills using appropriate techniques to protect personnel, work area and environment

6.4 Minimise generation of waste and environmental impacts

6.5 Collect and dispose of all wastes safely

6.6 Report hazards and incidents to designated personnel using enterprise procedures

Learning Outcomes

Details of Learning Activities

Practical Classes, question sheets, tutorials

Teaching Schedule

10.2.11 Introduction
Set up procedure
Ergonomic safety/OHS
Microscope theory
Microscope allocation
Workbook outline
 17.2.11Wet preps
Light characteristics
Cell types
Observation of yeasts
Drawing X100,X400Llabelled broth of sacchromyces
Onion slices
 24.2.11Parts of microscope,
Blood, Stains,
Storage of specimens
Labelling Making blood film-cells in blood- clinical
Blood film
Wright’s stain
Fixed smear observation
Wet prep
Slide mounting
 3.3.11Blood counts
Auto and manual counts on patient specimen*
(Prac report 20%)Due 18/3/11
 10.3.11Microscope trouble shooting
Tissue sections
Control slides
Oil introduction Measure cells
Perl’s stain prac
 17.3.11Individual tutorial Question sheet
Times to be announced
 24.3.11Prac assessment- wet prepsSet up wet prep on labelled sample according to industry procedure
9.5.11Gram stainGram Staining bacteria
30.5.11Assessment of oil immersion 
 week of 6.6.11 Theory test

Learning Resources

Prescribed Texts


Other Resources

Access to internet, library

Overview of Assessment

Assessments for this course typically consist of:
• Class activities
• Assignments
• Presentations
• Exams
  practical assessment

Assessment Tasks

Theory Test of underpinning knowledge  30%

Record of practicals done and questions in work book  50%


Practical write up  20%


Assessment of microscope set up and performance of wet preparation and oil immersion microscopy  Competent/ NYC

At least 5 of 7 practicals to be completed

Assessment Matrix

Other Information

All assessment tasks must be passed to pass the subject

The 40 hours allocated for this course are    

8X2 hour practical sessions                                 16 hours

1 report   research and writing                              4 hours

8 X 0.5 hour note reading and research                 4 hours

Completion of workbook and other questions         2.0X8 hour

2  X 1/4 hour tutorial sessions                              1 /2 hour

Practical assessments                                        1/2 hour

Theory assessment                                             1  hour


Course Overview: Access Course Overview