Course Title: Perform microscopic examination

Part B: Course Detail

Teaching Period: Term1 2014

Course Code: ONPS5656C

Course Title: Perform microscopic examination

School: 155T Vocational Health and Sciences

Campus: City Campus

Program: C5282 - Diploma of Laboratory Technology (Biotechnology)

Course Contact: Amberlee Mitton

Course Contact Phone: +61 3 9925 8053

Course Contact Email:

Name and Contact Details of All Other Relevant Staff

Gwen MacDonald

Nominal Hours: 40

Regardless of the mode of delivery, represent a guide to the relative teaching time and student effort required to successfully achieve a particular competency/module. This may include not only scheduled classes or workplace visits but also the amount of effort required to undertake, evaluate and complete all assessment requirements, including any non-classroom activities.

Pre-requisites and Co-requisites

there are no pre-requisites or co-requisites for this course


Course Description

This unit of competency covers the ability to set up a light microscope for optimum resolution, to prepare routine samples and to observe, identify and report sample characteristics.


National Codes, Titles, Elements and Performance Criteria

National Element Code & Title:

MSL973007A Perform microscopic examination


1. Interpret test requirements

Performance Criteria:

1.1 Review test request to identify samples to be tested, test method and equipment involved
1.2 Identify hazards associated with the sample, preparation methods, reagents and equipment and implement enterprise control measures


2. Set up work area for preparation and examination of samples

Performance Criteria:

 2.1 Check reagents are fit for purpose and report any items that require replacementCollect equipment and reagents and arrange the workspace so that equipment can be used safely and efficiently
2.2 Perform pre-use and safety checks to ensure equipment is fit for purpose and report faulty or unsafe equipment to appropriate personnel



3. Prepare samples for examination

Performance Criteria:

3.1 Log and label samples according to enterprise procedures to ensure traceability
3.2 Check suitability of the original and prepared sample for the examination and report unsuitable samples to appropriate personnel
3.3 Prepare and store the sample for examination following enterprise methods


4. Set up and use a light microscope

Performance Criteria:

4.1 Set up the light path to optimise resolution
4.2 Select the appropriate objectives and filter for the sample being examined
4.3 Ensure that the lenses are clean
4.4 Adjust settings and alignment of the light path to optimise performance
4.5 Place sample correctly on the stage


5. Observe, identify and report sample characteristics

Performance Criteria:

5.1 Recognise and identify significant sample characteristics
5.2 Perform required calculations accurately
5.3 Prepare and view control samples and check that results are consistent with expected values
5.4 Identify and report 'out of specification' or atypical results promptly to appropriate personnel
5.5 Record and report data in accordance with enterprise procedures


6. Maintain a safe work environment

Performance Criteria:

6.1 Ensure safety and minimise cross contamination through the use of personal protective clothing and safety equipment
6.2 Handle all samples and equipment in accordance with enterprise safety protocols
6.3 Clean up spills using appropriate techniques to protect personnel, work area and environment
6.4 Minimise generation of waste and environmental impacts
6.5 Collect and dispose of all wastes safely
6.6 Report hazards and incidents to designated personnel using enterprise procedures.

Learning Outcomes

Details of Learning Activities

Laboratory classes with short talks.

Lab manual questions relevant to prac work

On line materials and quizzes.

Tutorials with teachers

Teaching Schedule

Week beginningTheoryPrac



Introduction/OHSIntroduction to the microscope
17.2.14Use of microscope-wet preps
Labelling requirements
Yeast prac 
Storage of specimens
Stain theory
Blood film drawings
Measuring cells
Yeast wet prep
3.3.14Blood Counts, counting chambers, records, Quality ControlCWhole Blood Count auto and manual
Oil immersion
White Cell Count (manual)
17.3.14Individual tutorials
Question sheet
 Practical assessment completion
24.3.14Individual tutorials
Question sheet
 Practical assessment completion

Learning Resources

Prescribed Texts


Other Resources

access to library

Overview of Assessment

Assessments for this course typically consist of:
• Class activities
• Assignments
• Presentations
• Exams
  practical assessment

Assessment Tasks

Log book records and questions- submitted week 8 (CA/NYC)

On line test -  week 8   

Satisfactory performance of Microscopy skills. These may be assessed during classes and at final practical assessment

All assessment types must be passed to pass this course

Assessment Matrix

Other Information

80% of prac sessions need to be attended to pass this course.

This is pass only course- no higher grading available

Assessment Information

• To pass the course you need to pass each type of assessment. You can’t make up marks from one type of assessment to another (e.g. pass the tests but fail the log book).

• Extension of time for submission of assessable work- A student may apply for an extension of up to 7 days from the original due date. They must lodge the application form (available on the web ) at least the day before the due date. The application is lodged with the School Admin Office on Level 6, Bdg 51. Students requiring longer extensions must apply for Special Consideration (form available on the Web). For missed assessments such as exams- you (& your doctor if you are sick) must fill out a special consideration form. This form must be lodged at the HUB or online with supporting evidence (eg medical certificate), prior to, or within, 48 hours of the scheduled time of examination.

• Late work that is submitted without an application for an extension will not be corrected


Course Overview: Access Course Overview