Course Title: Prepare, standardise and use solutions

Part B: Course Detail

Teaching Period: Term2 2015

Course Code: ONPS5657C

Course Title: Prepare, standardise and use solutions

School: 155T Vocational Health and Sciences

Campus: City Campus

Program: C5282 - Diploma of Laboratory Technology (Biotechnology)

Course Contact: Amberlee Mitton

Course Contact Phone: +61 3 9925 8053

Course Contact Email:

Name and Contact Details of All Other Relevant Staff

Primary contact:
Kirsten Balding
9925 4283
Office 51.4.01
contact by email is the best option

Nominal Hours: 100

Regardless of the mode of delivery, represent a guide to the relative teaching time and student effort required to successfully achieve a particular competency/module. This may include not only scheduled classes or workplace visits but also the amount of effort required to undertake, evaluate and complete all assessment requirements, including any non-classroom activities.

Pre-requisites and Co-requisites

ONPS5658C Perform Chemical Tests

Course Description

This unit of competency covers the ability to prepare, standardise and monitor the quality of solutions.


National Codes, Titles, Elements and Performance Criteria

National Element Code & Title:

MSL974001A Prepare, standardise and use solutions


1. Prepare solutions

Performance Criteria:

1.1. Select appropriate procedure for solution preparation
1.2. Select equipment, materials and solvent of specified purity
1.3. Measure appropriate quantities of reagents for solution preparation and record data.
1.4. Select and assemble specified laboratory equipment and appropriate grade of glassware
1.5. Perform specified dilutions
1.6. Prepare solutions to achieve homogeneous mix of the specified concentration
1.7. Label and store solutions to maintain identity and stability


2. Standardise and use volumetric solutions

Performance Criteria:

2.1. Assemble appropriate laboratory equipment
2.2. Perform serial dilutions as required
2.3. Standardise the solution to the required specified range and precision
2.4. Label and store solutions to maintain identity and stability
2.5. Use standard volumetric solutions to determine concentration of unknown solutions


3. Calculate and record data

Performance Criteria:

3.1. Calculate specified concentrations
3.2. Use authorised procedure if data is to be modified
3.3. Estimate and document uncertainty of measurement in accordance with enterprise procedures, if required
3.4. Record all relevant details according to laboratory procedures and report results
3.5. Report concentration with appropriate units


4. Monitor the quality of laboratory solutions

Performance Criteria:

4.1. Check solutions for visual deterioration and expiry date
4.2. Restandardise or dispose of dated or deteriorated solutions
4.3. Record details and label solutions according to laboratory procedures


5. Maintain a safe work environment

Performance Criteria:

5.1. Use established safe work practices and personal protective equipment to ensure personal safety and that of other laboratory personnel
5.2. Clean up spills using appropriate techniques to protect personnel, work area and environment
5.3. Minimise generation of waste and environmental impacts
5.4. Ensure the safe collection of laboratory and hazardous waste for subsequent disposal
5.5. Store equipment and reagents as required

Learning Outcomes

Details of Learning Activities

Online theory presentations, worksheets and activities
whole class and group discussion
Online quizzes
Laboratory experiments
Record keeping of experiments
Research activities

Teaching Schedule

Prepare Solutions: 2015 Program
1.1: 7-Jul
Theory:Introduction to acids & bases, pH. Measuring pH, titration
Lab: Measuring pH: Indicators
Log Book only

1.2: 14-Jul
Theory: pH & pOH, Strong & weak acids & bases, pKa
Lab: Analysis of Vinegar by Titration
Lab Report 1: (done in class)

1.3: 21-Jul
Theory: Primary Standards and Standardisation of NaOH
Lab: Standardisation of NaOH
Labskills Assessment 1: Standardisation/Titration done in class

1.4: 28-Jul
Theory: Introduction to Buffers, Predicting pH of buffer solutions
Lab: measuring pH: the pH meter
Log Book only

1.5: 4-Aug
Theory: Buffering capacity, pH compared to pKa, buffer concentration
Lab: Buffers I - TAE, 1x TAE by dilution, Titration Curve
Log Book only

1.6: 11-Aug
(Lab only)
Lab: Buffers II - Phosphate buffers and the H-H equation
Log Book only

Revision (online)
Lab: (if required) Assessment catch up session:

Mid Semester exams

Week of 31 Aug: Mid semester Break

2.1: 8-Sep
(Theory at home): Intermolecular bonding, Water as a solvent,
Serial dilutions - refresher
2.2: 15-Sep
(Lab only)
Lab: Serial dilutions
Labskills Assessment: Serial dilutions

2.3: 22-Sep
Complex ions, Complexometric Titrations
Lab: Testing Hardness of Water

2.4: 29-Sep
(Lab only)
Lab: IDH Enzyme Assay
Lab report: IDH assay (due in 2 weeks)

2.5: 6-Oct
(Theory at home): Oxidation & Reduction (Redox) Reactions

2.6: 13-Oct
(Lab only)
Lab: Redox: Half cells and Electrochemical Series
Log Book: Submit logbook

Revision (at home)
Lab: (if required) Last chance Assessment catch up session
*Serial dilutions


Alternative assessment (Special Consideration only)

*subject to change

Learning Resources

Prescribed Texts

Bettelheim FA, Brown WH, Campbell MK, Farrell SO (2013) General, Organic and Biochemistry  10th Ed. Thomson/Brooks-Cole, Australia 

Lab Manual  - available from Blackboard

Blackboard for this course. Access through MyRMIT.


Other Resources

Lab coat, protective gloves, safety glasses, appropriate footwear & a logbook are required at all lab classes.

A logbook is required for this course to record all experimental details. The logbook must be brought to class each week and signed off by the teacher.
The book needs to be a bound (NON-SPIRAL) exercise book.


Overview of Assessment

Assessments for this course typically consist of:
• Class activities
• Assignments
• Presentations
• Exams

Assessment Tasks

Assessment Tasks

Assessment 1. Theory Exams

  • Exam 1: Term 3 topics only
  • Exam 2: Term 4 topics only

When: Week 8, Week 16
Value: 25%+25% = 50%

Assessment 2. Laboratory practice.
a. Labskills Assessments

  • 1. Standardisation and Titration
  • 2. Serial dilution

When: Weeks 3 and 10.
Satisfactory (Competent) completion required of both assessments

b. Lab Reports

  • 1. Titration of Vinegar
  • 2. Titration Curve

1. The whole experiment and report are completed in the week 2 class
2. The lab is done in week 12 and the report is due 2 weeks after the lab class (week 14)
Value: 15% + 15% = 30%

c. Logbook

  • Each week’s entry is to be signed off by student and teacher when it is considered ‘satisfactory’** and complete. Students must write up their logbook for at least 80% of labs. Only labs where the student was present may be written up. The checklist will collate this information. Submit the completed checklist at the end of semester. The logbook will be graded.

Complete logbook for all attended lab sessions
Submit logbook or completed checklist at end of semester
Pass grade required
Value: 20%

Assessment Matrix

Other Information

This course is graded in accordance with competency-based assessment, but which also utilise graded assessment
CHD Competent with High Distinction (80 – 100%)
CDI Competent with Distinction (70 – 79%)
CC Competent with Credit (60 – 69%)
CAG Competency Achieved – Graded (50 – 59%)
NYC Not Yet Competent
DNS Did Not Submit for assessment

• To pass the course you need to pass, on average, each type of assessment (exams, pracs and assignments etc.) For example, if there are two exams you need to have an average of 50% to pass and you also need to have passed the other assessment types. You can’t make up marks from one type of assessment to another (e.g. pass the exams but fail the prac component).

• Late work that is submitted without an application for an extension (see below) will not be corrected.

• APPLICATION FOR EXTENSION OF TIME FOR SUBMISSION OF ASSESSABLE WORK - A student may apply for an extension of up to 7 days from the original due date. They must lodge the application form (available online at least 24 hours before the due date. The application is lodged with the School Admin Office on Level 6, Bdg 51. Students requiring longer extensions must apply for SPECIAL CONSIDERATION.

• For missed assessments such as exams- you (& your doctor if you are sick) must fill out a special consideration form. This form must be lodged online with supporting evidence prior to, or within, 48 hours of the scheduled time of examination

Course Overview: Access Course Overview