Course Title: Prepare, standardise and use solutions

Part B: Course Detail

Teaching Period: Term2 2016

Course Code: ONPS5657C

Course Title: Prepare, standardise and use solutions

School: 155T Vocational Health and Sciences

Campus: City Campus

Program: C5282 - Diploma of Laboratory Technology (Biotechnology)

Course Contact: Amberlee Mitton

Course Contact Phone: +61 3 9925 8053

Course Contact Email:

Name and Contact Details of All Other Relevant Staff

Primary contact:
Kirsten Balding
9925 4283
Office 51.4.01
contact by email is the best option

Nominal Hours: 100

Regardless of the mode of delivery, represent a guide to the relative teaching time and student effort required to successfully achieve a particular competency/module. This may include not only scheduled classes or workplace visits but also the amount of effort required to undertake, evaluate and complete all assessment requirements, including any non-classroom activities.

Pre-requisites and Co-requisites

ONPS5658C Perform Chemical Tests

Course Description

This unit of competency covers the ability to prepare, standardise and monitor the quality of solutions.


National Codes, Titles, Elements and Performance Criteria

National Element Code & Title:

MSL974001A Prepare, standardise and use solutions


1. Prepare solutions

Performance Criteria:

1.1. Select appropriate procedure for solution preparation 1.2. Select equipment, materials and solvent of specified purity 1.3. Measure appropriate quantities of reagents for solution preparation and record data. 1.4. Select and assemble specified laboratory equipment and appropriate grade of glassware 1.5. Perform specified dilutions 1.6. Prepare solutions to achieve homogeneous mix of the specified concentration 1.7. Label and store solutions to maintain identity and stability


2. Standardise and use volumetric solutions

Performance Criteria:

2.1. Assemble appropriate laboratory equipment 2.2. Perform serial dilutions as required 2.3. Standardise the solution to the required specified range and precision 2.4. Label and store solutions to maintain identity and stability 2.5. Use standard volumetric solutions to determine concentration of unknown solutions


3. Calculate and record data

Performance Criteria:

3.1. Calculate specified concentrations 3.2. Use authorised procedure if data is to be modified 3.3. Estimate and document uncertainty of measurement in accordance with enterprise procedures, if required 3.4. Record all relevant details according to laboratory procedures and report results 3.5. Report concentration with appropriate units


4. Monitor the quality of laboratory solutions

Performance Criteria:

4.1. Check solutions for visual deterioration and expiry date 4.2. Restandardise or dispose of dated or deteriorated solutions 4.3. Record details and label solutions according to laboratory procedures


5. Maintain a safe work environment

Performance Criteria:

5.1. Use established safe work practices and personal protective equipment to ensure personal safety and that of other laboratory personnel 5.2. Clean up spills using appropriate techniques to protect personnel, work area and environment 5.3. Minimise generation of waste and environmental impacts 5.4. Ensure the safe collection of laboratory and hazardous waste for subsequent disposal 5.5. Store equipment and reagents as required

Learning Outcomes

Details of Learning Activities

Online theory presentations, worksheets and activities
Whole class and group discussion
Online quizzes
Laboratory experiments
Record keeping of experiments
Research activities

Teaching Schedule



Week No.


(Week of)







1.1 Introduction to acids & bases, pH. Measuring pH, titration

Lab 1
Measuring pH: Indicators

Make anhydrous KHP

Log Book & Lab Skills



1.2: Titration, pH & pOH, Strong & weak acids & bases, pKa,
Intro to standardisation Titrating accurately and precisely – Standard & Random errors

Lab 2 Analysis of Vinegar by Titration

Do 1 go at standardization

Log Book & Lab Skills




1.3 Primary Standards and Standardisation of NaOH

Lab 3

Add prepare 0.2M NaOH from a stock solution
Standardization 1: NaOH/KHP

Log Book & Lab Skills

Standardisation 1




1.4 Introduction to Buffers, Predicting pH of buffer solutions

Lab 4
Measuring pH:
the pH meter

Log Book & Lab Skills



1.5 Buffering capacity, pH compared to pKa, buffer concentration,
Titration Curves

Lab 5
1. Buffers I: 1x TAE
by dilution

2. Titration curve

Log Book & Lab Skills


Titration Curve


Lab Report 1
(done in class)



1.6 Predicting buffer pH
(Lab only)

Lab 6 Buffers II – Phosphate buffers and the H-H equation

Log Book & Lab Skills


Submit logbook & checklist by 5pm Friday for midsemester review



(at home)

Last chance Assessment catch up session*
Lab 3Standardization 1: NaOH/KHP

Lab 5Titration curve
NOTE: There is not enough time to catch up both assessments

Lab Skills
(Catch up:Lab 3 OR 5)




Mid Semester exams


Theory Exam 1


 Week of 29 Aug

Mid semester Break



Subject to change.

* Assessment catch ups areonly for people with a medical certificate or special consideration granted, or who didn’t meet the precision and accuracy requirements on their 1st attempt.




Term 4

Week No.


(Week of)












2.1 Serial dilutions – refresher


Lab 7Serial dilutions Spectrophotometry

Log Book & Lab Skills

Serial Dilution
Lab Report 2
(done in class)



2.2 Complex ions and Complexometric titrations model building
in-class activity

Last chance Assessment catch up session*
Lab 7

(Catch up:Lab 7)




2.3 (Lab Only)

Lab 8 
Testing hardness of water

Standardization 2: EDTA/CaCO3

Log Book & Lab Skills

Standardisation 2





2.4 Oxidation & Reduction (Redox) Reactions
(Theory at home)

Last chance Assessment catch up session*
Lab 8 

(Catch up:Lab 8)



2.5 (Lab Only)

Lab 9Redox: Half cells and Electrochemical Series

Log Book & Lab Skills




2.6 (Lab only)

Lab 10Redox titration: Vitamin C in Fruit with Standardization 3: KI

Log Book & Lab Skills

Standardisation 3

Submit logbook & checklist by 5pm Friday



Revision (at home)

Last chance Assessment catch up session*
Lab 10

(Catch up:Lab 10)






Theory Exam 2



Alternative assessment (special consideration only)


Subject to change.

* Assessment catch ups areonly for people with a medical certificate or special consideration granted, or who didn’t meet the precision and accuracy requirements on their 1st attempt.


Learning Resources

Prescribed Texts

Bettelheim FA, Brown WH, Campbell MK, Farrell SO (2015) General, Organic and Biochemistry  11th Ed. Thomson/Brooks-Cole, Australia 

Lab Manual  - available from Blackboard

Blackboard for this course. Access through MyRMIT.


Other Resources

Lab coat, protective gloves, safety glasses, appropriate footwear & a logbook are required at all lab classes.

A logbook is required for this course to record all experimental details. The logbook must be brought to class each week and signed off by the teacher.
The book needs to be a bound (NON-SPIRAL) exercise book.

A permanent marker for writing on glassware


Overview of Assessment

Assessments for this course typically consist of:
• Class activities
• Assignments
• Presentations
• Exams

Assessment Tasks





Assessment 1. Theory Exams

         Exam 1: Part 1 topics only

         Exam 2: Part 2 topics only


Week 8

Week 16



= 50%

Assessment 2. Laboratory practice

  1. Logbook Records
    Students must attend and record at least 80% of the labs. Records must be finalized within 2 weeks of the experiment.


Complete logbook for all attended lab sessions

Logbook and Labskills checklist must be signed off within 2 weeks of each class.

Submit logbook & checklist by Friday 5pm of week 6 (mid semester review) and week 14 (final assessment)

20 %

  1. Labskills

Lab skills will be assessed each week along with the logbook records using the ‘logbook & labskills checklist’.

To pass the course students MUST
attend 80% of labs AND:

  1. Standardisethree (3) different solutions to the requiredprecision and accuracy
  2. Do a titration using each of the threestandardised solution to the required precision and accuracy
  3. Complete a serial dilution to the required precision and accuracy
  4. Create a titration curve and
    use it to identify the equivalence point of a titrationtothe required precision and accuracy

The specific tasks outlined at left are done in Weeks 3, 5, 9, 11, 14

One catch up session* is available for each task. Dates of the catch up sessions are shown in the class schedule. Only people with special consideration or another documented valid reason (such as a medical certificate) can attend these catch up sessions.

Satisfactory at all tasks

  1. 2 Lab Reports
  2. Titration curve
  3. Serial dilution spectrophotometry

The experiments and their report are completed in
(1) the week 5 class and (2) the week 9 class

= 30%



100 %





*Catch up sessions are only available for students with a medical certificate, or equivalent supporting documentation, or special consideration.

**See note for further information


**Students must record logbook entries for at least 80% of Lab classes AND pass all assessments to pass the course**.

Assessment Matrix

Other Information

This course is graded in accordance with competency-based assessment, but which also utilise graded assessment
CHD Competent with High Distinction (80 – 100%)
CDI Competent with Distinction (70 – 79%)
CC Competent with Credit (60 – 69%)
CAG Competency Achieved – Graded (50 – 59%)
NYC Not Yet Competent
DNS Did Not Submit for assessment

• To pass the course you need to pass, on average, each type of assessment (exams, pracs and assignments etc.) For example, if there are two exams you need to have an average of 50% to pass and you also need to have passed the other assessment types. You can’t make up marks from one type of assessment to another (e.g. pass the exams but fail the prac component).

• Late work that is submitted without an application for an extension (see below) will not be corrected.

• APPLICATION FOR EXTENSION OF TIME FOR SUBMISSION OF ASSESSABLE WORK - A student may apply for an extension of up to 7 days from the original due date. They must lodge the application form (available online at least 24 hours before the due date. The application is lodged with the School Admin Office on Level 6, Bdg 51. Students requiring longer extensions must apply for SPECIAL CONSIDERATION.

• For missed assessments such as exams- you (& your doctor if you are sick) must fill out a special consideration form. This form must be lodged online with supporting evidence prior to, or within, 48 hours of the scheduled time of examination



What does “all assessments must be passed” mean exactly?

It is your responsibility to understand exactly what you need to do to pass the course, but hopefully the information below will make things clear… if you need more information, please ask a teacher J


This means you need to pass, on average, each type of assessment listed in the table ie:

  • Average exam mark (2 exams) is a pass,
  • Average Lab report mark (2 reports) is a pass,
  • Satisfactory completion of all required Lab Skills (outlined below) AND
  • Logbook record keeping is a pass.

If you miss a Lab class you need to provide a copy of your medical certificate or other evidence (court appearance, funeral notice, statutory declaration) to the teacher.

Quizzes are optional in this course.
The quizzes are designed to give you feedback about how well you are understanding the material. Therefore it is highly recommended that you complete the quizzes.

Students must attend and record at least 80% of the labs. Records must be finalized within 2 weeks of the experiment. Students must write up their logbook for all labs that they attend to show that they are proficient at recording experiments in a timely manner.
The marking guide shows how the mark will be generated. Only records produced within 2 weeks of the experiment will be graded. Students can self-assess their logbook records using the logbook checklist.

A requirement of this assessment task is that students collect evidence that they have prepared solutions and collected data that shows each solution is fit for purpose. Only some experiments are suitable for this purpose (see checklist). At least 1 of the suitable experiments needs to be amongst those recorded to meet the criteria.

If you are absent or have a medical certificate or other acceptable paperwork to cover an absence, this should be recorded in the logbook checklist.


Lab Reports

If you miss either of the Lab report classes you will only be eligible to write up/catch up the Lab if you provide a medical certificate or if special consideration is granted. For this to occur you need to discuss your situation with the teacher as soon as possible. Other reasons for missing a write up Lab do not mean that you can write up a different report.

Lab reports are due in the session where the activity is done.
There is a document for you to fill out for the report.


Lab Skills Assessment

Lab skills are assessed each week. As well as attending 80% of classes, particular skills must be achieved with the results generated to the required accuracy and precision. The required procedures are: Three solutions must be standardized and used in its titration, a titration curve must be produced and used to find an equivalence point, and a serial dilution done and verified by spectrophotometry. Accuracy & Precision requirements will be defined during each lab class using class data.

If you miss an assessment session note that catch up sessions are only available to students if a medical certificate for the original assessment date or other evidence (court appearance, funeral notice, statutory declaration) has been presented. Note some catch up days are in the revision weeks, so it is to your advantage to attend the original classes if possible to give yourself more time to revise for the exam.

Course Overview: Access Course Overview