Course Title: Perform biological procedures

Part B: Course Detail

Teaching Period: Term1 2013

Course Code: ONPS5659C

Course Title: Perform biological procedures

School: 155T Vocational Health and Sciences

Campus: City Campus

Program: C5282 - Diploma of Laboratory Technology (Biotechnology)

Course Contact: Amberlee Mitton

Course Contact Phone: +61 3 9925 8053

Course Contact Email:

Name and Contact Details of All Other Relevant Staff

Teacher: Amberlee Mitton

Phone # 9925 8053

Office: 51.4.01

Nominal Hours: 130

Regardless of the mode of delivery, represent a guide to the relative teaching time and student effort required to successfully achieve a particular competency/module. This may include not only scheduled classes or workplace visits but also the amount of effort required to undertake, evaluate and complete all assessment requirements, including any non-classroom activities.

Pre-requisites and Co-requisites


MSL973004A - Perform aseptic techniques  AND  MSL973007A - Perform microscopic examination 


Course Description

This unit of competency covers the ability to interpret work requirements, prepare samples, conduct pre-use and calibration checks on equipment and perform routine biological procedures, including sample preparation. These procedures may involve several steps and are used to classify cell types, species and biologically active compounds by analysing their biological and chemical characteristics. This unit includes data processing, interpretation of results and troubleshooting obvious departures from standard procedures.


National Codes, Titles, Elements and Performance Criteria

National Element Code & Title:

MSL974006A Perform biological procedures


1. Interpret and schedule work requirements

Performance Criteria:

1.1. Review work request to identify samples, required procedures and materials/equipment/instruments involved
1.2. Identify hazards and enterprise control measures associated with the sample, preparation methods, reagents and/or equipment
1.3. Plan parallel work sequences to optimise throughput of multiple sets of samples, if appropriate


2. Receive and prepare biological samples

Performance Criteria:

2.1. Log samples using standard operating procedures (SOPs)
2.2. Record sample description, compare with specification and note and report discrepancies
2.3. Prepare samples in accordance with testing requirements
2.4. Ensure traceability of sample from receipt to reporting of results


3. Perform techniques that assist in the classification of a cell or species

Performance Criteria:

3.1. Select suitable techniques in accordance with enterprise requirements and methods
3.2. Set up and use equipment and reagents in accordance with the method
3.3. Perform techniques in accordance with the method


4. Perform techniques that analyse biological activity

Performance Criteria:

4.1. Select suitable techniques in accordance with enterprise requirements and methods
4.2. Set up and use equipment and reagents in accordance with the method
4.3. Perform techniques in accordance with the method


5. Process and interpret data

Performance Criteria:

5.1. Record test data noting atypical observations
5.2. Construct calibration graphs, if appropriate, and compute results for all samples from these graphs
5.3. Ensure calculated values are consistent with expectations
5.4. Record and report results in accordance with enterprise procedures
5.5. Estimate and document uncertainty of measurement in accordance with enterprise procedures, if required
5.6. Interpret trends in data and/or results and report out of specification or atypical results promptly to appropriate personnel
5.7. Determine if obvious procedure or equipment problems have led to atypical data or results


6. Maintain a safe work environment

Performance Criteria:

6.1. Use established safe work practices and personal protective equipment to ensure personal safety and that of other laboratory personnel
6.2. Minimise the generation of wastes
6.3. Ensure the safe disposal of biohazardous wastes
6.4. Clean, care for and store equipment and reagents as required


7. Maintain laboratory records

Performance Criteria:

7.1. Record approved data into enterprise system
7.2. Maintain confidentiality and security of enterprise information and laboratory data
7.3. Maintain equipment and calibration logs in accordance with enterprise procedures

Learning Outcomes

Details of Learning Activities

• Teacher directed delivery of lessons
• Class discussion
• Worksheets
• Laboratory experiments
• Record keeping of experiments
• Research activities
• Site Visits

Teaching Schedule


Wk. no. Week Beggining  TopicActivity/Practical Assessment1234567a7b8 91011121314151617
Mon 11th FebTopic 1 : A view of life
Mon 18th FebTopic 2 : Chemistry of lifePractical 1: Enzymes (Wet or online) 
Mon 25th FebTopic 3 : Cell structure and functionActivity: Post- IT Cell 
Mon 4th MarTopic 4 : Cell transportPractical 2: Osmosis and diffusion Assessment 1: Practical report: Osmosis and diffusion (7.5%)
DUE Date: Week 6
Mon 11th MarTopic 5 : Cell reproduction Activity: Mitosis under the microscope 
Mon 18th MarTopic 6 : Ecology 1  
Mon 25th Mar Student Vacation
Mon 1st Apr RevisionSite visit: Royal Botanical Gardens Assessment 2 : Site visit report (7.5%)
DUE Date: Week 10
Mon 8th Apr Exam Week  Assessment 3: Mid semester exam (12.5%)
Mon 15th Apr Topic 7 : Ecology 2  
Mon 22nd Apr Topic 8 : Respiration and photosynthesisPractical 3: Floating leaf (photosynthesis) 
Mon 29th Apr Topic 9 : The immune system 1 (Normal response to trauma and infection & anatomy of the immune system)  
Mon 6th May Topic 10 : The immune system 2 (Killing invaders, cell mediated immunity & humoral immunity)  
Mon 13th May Topic 11 : The Circulatory SystemPractical: Sheep heart dissection 
Mon 20th May Topic 12 : The Respiratory SystemActivity: Building a Model of the Respiratory System 
Mon 27th May RevisionLibrary class: Virtual rat dissection 
Mon 3rd Jun End of Semester Exam
Assessment 4: End of Semester Exam (12.5%)

Learning Resources

Prescribed Texts

Introduction to General, Organic & Biochemistry (9th Edition) Bettelheim, Brown & March


Other Resources

Overview of Assessment

Assessments for this course typically consist of:
• Class activities
• Assignments
• Presentations
• Exams

Assessment Tasks

Assessment 1: Practical report: Osmosis and diffusion (7.5%)
DUE Date: Week 6

Assessment 2 : Site visit report (7.5%)
DUE Date: Week 10

Assessment 3: Mid semester exam (12.5%)

Assessment 4: End of Semester Exam (12.5%)

Please note: This is a year long subject

Assessment Matrix

Other Information

Assessment Information

• To pass the course you need to pass, on average, each type of assessment (exam, prac reports, and assignments etc.). For example, if there are two tests you need to have an average of 50% to pass. But you also need to have passed the other assessments as well. You can’t make up marks from one type of assessment to another (eg pass the tests but fail the prac reports).

• Late work that is submitted without an application for an extension will not be corrected.

• Extension of time for submission of assessable work- A student may apply for an extension of up to 7 days from the original due date. They must lodge the application form (available on the web ) at least the day before the due date. The application is lodged with the School Admin Office on Level 6, Bdg 51. Students requiring longer extensions must apply for Special Consideration (form available on the Web). For missed assessments such as exams- you (& your doctor if you are sick) must fill out a special consideration form. This form must be lodged at the HUB or online with supporting evidence (eg medical certificate), prior to, or within, 48 hours of the scheduled time of examination.

• In order to be successful in this module, you have to achieve a pass in all of the above assessment tasks. If you miss an assessment task due to unavoidable circumstances, you need to follow the procedure of special consideration and apply within the allowed time frame.

If you have any questions about this assessment process, feel free to email me on


Course Overview: Access Course Overview