Course Title: Perform biological procedures
Part B: Course Detail
Teaching Period: Term1 2016
Course Code: ONPS5659C
Course Title: Perform biological procedures
School: 155T Vocational Health and Sciences
Campus: City Campus
Program: C5282 - Diploma of Laboratory Technology (Biotechnology)
Course Contact: Amberlee Mitton
Course Contact Phone: +61 3 9925 8053
Course Contact Email: amberlee.mitton@rmit.edu.au
Name and Contact Details of All Other Relevant Staff
Teacher: Amberlee Mitton
Phone: 9925 8053
Office: 51.4.01
Nominal Hours: 130
Regardless of the mode of delivery, represent a guide to the relative teaching time and student effort required to successfully achieve a particular competency/module. This may include not only scheduled classes or workplace visits but also the amount of effort required to undertake, evaluate and complete all assessment requirements, including any non-classroom activities.
Pre-requisites and Co-requisites
Pre-Requisites
MSL973004A - Perform aseptic techniques AND MSL973007A - Perform microscopic examination
Course Description
This unit of competency covers the ability to interpret work requirements, prepare samples, conduct pre-use and calibration checks on equipment and perform routine biological procedures, including sample preparation. These procedures may involve several steps and are used to classify cell types, species and biologically active compounds by analysing their biological and chemical characteristics. This unit includes data processing, interpretation of results and troubleshooting obvious departures from standard procedures.
National Codes, Titles, Elements and Performance Criteria
National Element Code & Title: |
MSL974006A Perform biological procedures |
Element: |
1. Interpret and schedule work requirements |
Performance Criteria: |
1.1.Review work request to identify samples, required |
Element: |
2. Receive and prepare biological samples |
Performance Criteria: |
2.1.Log samples using standard operating procedures |
Element: |
3. Perform techniques that assist in the classification of a cell or species |
Performance Criteria: |
3.1.Select suitable techniques in accordance with |
Element: |
4. Perform techniques that analyse biological activity |
Performance Criteria: |
4.1.Select suitable techniques in accordance with |
Element: |
5. Process and interpret data |
Performance Criteria: |
5.1.Record test data noting atypical observations |
Element: |
6. Maintain a safe work environment |
Performance Criteria: |
6.1.Use established safe work practices and personal |
Element: |
7. Maintain laboratory records |
Performance Criteria: |
7.1.Record approved data into enterprise system |
Learning Outcomes
Details of Learning Activities
• Teacher directed delivery of lessons
• Class discussion
• Worksheets
• Laboratory experiments
• Record keeping of experiments
• Research activities
• Site Visits
Teaching Schedule
Semester 1
- Week 1 Topic 1: A view of life Intro to course/assessment items and blackboard tour, Activity 1a: M&M’s and the Scientific Method & Activity 1b: Exploring the scientific method
- Week 2 Topic 2: Ecology 1, Activity 2: Food webs/chains and carbon cycle
- Week 3 Topic 3: Ecology 2 Activity 3: The Lesson of the Kaibab, Site visit planning and report writing tutorial
- Week 4 Site visit Compulsory to attended and collect data for Assessment 1, Activity 4: Site visit to Royal Botanical Gardens, Assessment 1: Site visit report (7.5 %)
- Week 5 Report writing, Turnitin and referencing tutorial
- Week 6 Topic 4: Photosynthesis, Practical 1: Floating leaf (photosynthesis)
- Week 7a No class
- Week 7b No class Student Break
- Week 8 Assessment week - No test or class
- Week 9 Topic 5: Cellular respiration, Activity 5: Cellular respiration story board
- Week 10 Topic 6 Cell structure and function & Assessment 2 preparation
- Week 11 Topic 7: Cell transport, Compulsory to attended and collect data for Assessment 2, Assessment 2: Practical report: Diffusion of glucose/starch across a membrane (7.5%)
- Week 12 Topic 8: Cell reproduction, Prac 2 - : Mitosis under the microscope
- Week 13 Topic 9: The immune system 1 (Normal response to trauma and infection & anatomy of the immune system), Prac 4: Inflammation and phagocytosis
- Week 14 Topic 10: The immune system 2 (Killing invaders, cell mediated immunity & humoral immunity), Activity 7: Case study analysis and class discussion
- Week 15 Mon 25th May Revision, Practice test discussion
- Week 16 Mon 1st Jun Assessment week, Assessment 3: Test 1 – Topic 1 – 12 (15%)
Semester 2
Week 1 Introduction to Biomolecules,Structure and function: Nucleic acids
PRAC 1 - Preparing solutions-Biuret & Saturated SAS & Risk assessments
Week 2 DNA replication
PRAC 2 - Mini Prep (Kit) + RE digest OR PRAC 3 - Electrophoresis of Dyes
Assessment 4 - Practical report Electrophoresis of dyes (Due week 4) 10%
Week 3 Structure and function: Proteins
Prac 2 - Mini Prep (Kit) + RE digest OR PRAC 3 - Electrophoresis of Dyes
Assessment 4 - Practical report Electrophoresis of dyes (Due week 5)
Week 4 Structure and function: Enzymes
PRAC 4 - Spectrophotometric Protein Determination Biuret Method (5 point curve)
Week 5 Structure and function: Carbohydrates
PRAC 5a - Rapid Spectrophotometric Protein Determination Biuret Method (1 point curve and Calculation to determine final protein concentration)
Week 6 Structure and function: Lipids
PRAC 6 - Enzyme Lab
& PRAC 5b - Rapid Spectrophotometric Protein Determination Biuret Method (Calculation only to determine final protein concentration)
Week 7 Revision
PRAC 5c - Rapid Spectrophotometric Protein Determination Biuret Method (Calculation only to determine final protein concentration)
& Peer review of log books
Week 8 Exam week Assessment 5: Test 2 (25%)
Student vacation
Week 9 The Biochem laboratory and techniques
PRAC 6 - Carbohydrates
& PRAC 5d - Rapid Spectrophotometric Protein Determination Biuret Method (Calculation only to determine final protein concentration)
Week 10 Transcription
PRAC 7 - Fractionation of Milk
& PRAC 5e - Rapid Spectrophotometric Protein Determination Biuret Method (Calculation only to determine final protein concentration)
Assessment 6: Practical report Rapid Biuret Protein Determination 10%
Week 11 Translation
Practical Test: Rapid Spectrophotometric Protein Determination Biuret Method (Calculation only to determine final protein concentration)
Assessment 7 - Practical Test: Rapid Biuret Protein (S/NYS)
Week 12 Clinical Biochemistry 1
PRAC 8 - DNA Restriction enzyme digest electrophoresis (using plasmid RE digest from mini prep)
OR PRAC 9 - Spectrophotometric determination of DNA
Week 13 Clinical Biochemistry 2
PRAC 8 - DNA electrophoresis (using plasmid RE digest from mini prep)
OR PRAC 9 - Spectrophotometric determination of DNA
Week 14 Chemical communications: neurotransmitters & Logbook assessments
Week 15 Revision
Supplementary practical test & Log book grading (face to face)
Assessment 8: Log Book (S/NYS)
Week 16 Exam Week Assessment 9: Test 3 (25%)
Learning Resources
Prescribed Texts
Introduction to General, Organic & Biochemistry (10th Edition) Bettelheim, Brown & March |
References
Other Resources
Overview of Assessment
Assessments for this course typically consist of:
• Class activities
• Assignments
• Presentations
• Exams
Assessment Tasks
Semester 1
Assessment 1: Site visit report (7.5 %) Week 4
Assessment 2: Practical report: Diffusion of glucose/starch across a membrane (7.5%) Week 11
Assessment 3: Test 1 Topic 1 – 12 (15%) Week 16
Semester 2
Assessment 4: Practical report Electrophoresis of dyes (10%) Due week 5
Assessment 5: Test 2 (25%) Week 8
Assessment 6: Practical report Rapid Biuret Protein Determination 10% Due week 10
Assessment 7: Practical Test: Rapid Biuret Protein (S/NYS) Week 11
Assessment 8: Log Book (S/NYS)
Assessment 9: Test 3 (25%) Week 16
*Detailed outlines and instructions on blackboard
Assessment Matrix
See Blackboard
Other Information
Assessment Information
• This course is graded in accordance with competency-based assessment, but which also utilise graded assessment
CHD Competent with High Distinction (80 – 100%)
CDI Competent with Distinction (60 – 79%)
CC Competent with Credit (50 – 59%)
CAG Competency Achieved – Graded (0 – 49%)
NYC Not Yet Competent
DNS Did Not Submit for assessment
• To pass the course you need to pass, on average, each type of assessment (exams, pracs, and assignments etc.) For example, if there are two exams you need to have an average of 50% to pass and you also need to have passed the other assessment types. You can’t make up marks from one type of assessment to another (e.g. pass the exams but fail the prac component).
• Late work that is submitted without an application for an extension (see below) will not be corrected.
• APPLICATION FOR EXTENSION OF TIME FOR SUBMISSION OF ASSESSABLE WORK - A student may apply for an extension of up to 7 days from the original due date. They must lodge the application form (available online http://www1.rmit.edu.au/students/assessment/extension) at least 24 hours before the due date. The application is lodged with the School Admin Office on Level 6, Bdg 51. Students requiring longer extensions must apply for SPECIAL CONSIDERATION.
• For missed assessments such as exams- you (& your doctor if you are sick) must fill out a special consideration form. This form must be lodged online with supporting evidence prior to, or within, 48 hours of the scheduled time of examination http://www1.rmit.edu.au/students/specialconsideration
Plagiarism is the presentation of the work, idea or creation of another person as though it is your own. It is a form of cheating and is a very serious academic offence that may lead to expulsion from the University. Plagiarised material can be drawn from, and presented in, written, graphic and visual form, including electronic data and oral presentation. Plagiarism occurs when the origin of the material used is not appropriately cited. It also occurs through enabling plagiarism, which is the act of assisting or allowing another person to plagiarise or to copy your own work. Please make sure you consider this carefully in completing all your work and assessments in this course and if you are unsure about whether you might have plagiarised, seek help from your teacher.
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