Course Title: Prepare tissue and cell cultures
Part B: Course Detail
Teaching Period: Term1 2014
Course Code: ONPS5661C
Course Title: Prepare tissue and cell cultures
School: 155T Vocational Health and Sciences
Campus: City Campus
Program: C5282 - Diploma of Laboratory Technology (Biotechnology)
Course Contact: Amberlee Mitton
Course Contact Phone: +61 3 9925 8053
Course Contact Email: amber.mitton@rmit.edu.au
Name and Contact Details of All Other Relevant Staff
Teacher: Chris Ozga
Nominal Hours: 50
Regardless of the mode of delivery, represent a guide to the relative teaching time and student effort required to successfully achieve a particular competency/module. This may include not only scheduled classes or workplace visits but also the amount of effort required to undertake, evaluate and complete all assessment requirements, including any non-classroom activities.
Pre-requisites and Co-requisites
ONPS5655C Perform Aseptic techniques
ONPS 5656C Microscopy
Course Description
This unit of competency covers the ability to prepare primary tissue cultures for applications, such as maintenance of animal cell lines and propagation of plants by tissue culture and basic subculture procedures. Personnel are required to manipulate equipment and materials and samples to prevent contamination at all preparation stages. They will have ready access to enterprise procedures and will work under direct supervision.
National Codes, Titles, Elements and Performance Criteria
National Element Code & Title: |
MSL974011A Prepare tissue and cell cultures |
Element: |
1. Work safely according to the legal and regulatory framework |
Performance Criteria: |
1.1. Ensure work practices and personal actions conform to regulations, codes, guidelines and enterprise quality assurance procedures |
Element: |
2.Prepare and test cell and tissue culture media |
Performance Criteria: |
2.1. Select and confirm media specifications and processes/methods |
Element: |
3. Prepare tissue or cell cultures |
Performance Criteria: |
3.1. Select tissue/cell sample to optimise growth and prepare it for culture |
Element: |
4. Monitor tissue or cell culture |
Performance Criteria: |
4.1. Incubate culture in specified conditions |
Element: |
5. Maintain records |
Performance Criteria: |
5.1. Maintain records of batches of media and test data |
Learning Outcomes
Details of Learning Activities
- Teacher directed delivery of lessons
- Whole class discussion
- Pair/group discussion and self assessment
- Worksheets
- Laboratory experiments
- Record keeping of experiments
- Research activities
Teaching Schedule
Topic 1: Intro to Biohazard cabinets, aseptic technique and tissue culture | PRAC 1: Introduction to the Biohazard hood, aseptic technique and tissue culture |
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Topic 2: Introduction to plant tissue culture & plant physiology |
PRAC 2: Seed (Lepidium sativum) propagation PRAC 3: Media preparation |
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Topic 3: Plant growth |
PRAC 4: Lepidium sativum seedling transfer PRAC 5: Cauliflower explant |
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Topic 4: Plant reproduction |
PRAC 6: Surface sterilisation preparation Collect PRAC 5 results |
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Topic 5: Tissue culture environment | PRAC 7: Surface sterilisation and explant of African Violet leaves | |
Topic 6: Media & Growth Regulators | PRAC 8: Effect of surface sterilisation and explant of Petunia leaves & Collect results from prac 6 |
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PRAC 9: Collect and compare results from PRAC 6 and 7
Discussion of PRAC results and Assessment 1 |
Assessment 1: Prac report 1 (PRAC 6, 7, 8 & 9) DUE: Week 10b (30%) |
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Exam Week | ||
Topic 7: Micropropagation 1 | PRAC 10: Carrot callus culture | |
Topic 8: Micropropagation 2 | Library research for group presentation | |
Student Vacation | PRAC 6: | |
Topic 9: Contamination | PRAC 11: Succulent explant and collect PRAC 10 results (3 weeks) | |
Topic 10 - GMO | PRAC 12: Collect PRAC 10 (4 weeks) & PRAC 11 results | |
Presentation prep |
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Revision | Activity 4 – Group presentations PRAC 13: Collect PRAC 11 results and take plants home |
Assessment 2: Group presentation: |
Practical exam (Explant) | Assessment 3: Practical Exam – Competency based assessment (C/NYC) (Technical skills)
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End of Semester Exam | No Practical | Assessment 5: End of year quiz (40%) |
Learning Resources
Prescribed Texts
References
Other Resources
Overview of Assessment
Asessment for this course may include tests, practical reports, assignments and presentations
Assessment Tasks
Assessment 1: Prac report (30%)
Assessment 2: Group presentation (30%)
Assessment 3: Practical Exam ( C/NYC)
Assessment 4: Log Book (C/NYC)
Assessment 5: End of year quiz (40%)
Assessment tasks will assess the student’s ability to demonstrate the following competencies:
- OH&S requirements and code of conduct in a tissue culture laboratory
- Ability to prepare reagents and tissue culture media accordingly
- Culturing cells aseptically
- Monitoring the growth of cultures and distinguishing any normal from abnormal cells as well as contamination
- Proper record maintenance and analysis of results
Assessment Matrix
Other Information
Assessment Information
• To pass the course you need to pass, on average, each type of assessment (exam, prac reports, and assignments etc.). For example, if there are two tests you need to have an average of 50% to pass. But you also need to have passed the other assessments as well. You can’t make up marks from one type of assessment to another (eg pass the tests but fail the prac reports).
• Late work that is submitted without an application for an extension will not be corrected.
• Extension of time for submission of assessable work- A student may apply for an extension of up to 7 days from the original due date. They must lodge the application form (available on the web http://mams.rmit.edu.au/seca86tti4g4z.pdf ) at least the day before the due date. The application is lodged with the School Admin Office on Level 6, Bdg 51. Students requiring longer extensions must apply for Special Consideration (form available on the Web). For missed assessments such as exams- you (& your doctor if you are sick) must fill out a special consideration form. This form must be lodged at the HUB or online with supporting evidence (eg medical certificate), prior to, or within, 48 hours of the scheduled time of examination.
• If you miss an assessment task due to unavoidable circumstances, you need to follow the procedure of special consideration and apply within the allowed time frame.
If you have any questions about this assessment process, feel free to email your teacher
Course Overview: Access Course Overview