Course Title: Prepare tissue and cell cultures

Part B: Course Detail

Teaching Period: Term1 2014

Course Code: ONPS5661C

Course Title: Prepare tissue and cell cultures

School: 155T Vocational Health and Sciences

Campus: City Campus

Program: C5282 - Diploma of Laboratory Technology (Biotechnology)

Course Contact: Amberlee Mitton

Course Contact Phone: +61 3 9925 8053

Course Contact Email: amber.mitton@rmit.edu.au


Name and Contact Details of All Other Relevant Staff

Teacher: Chris Ozga

Nominal Hours: 50

Regardless of the mode of delivery, represent a guide to the relative teaching time and student effort required to successfully achieve a particular competency/module. This may include not only scheduled classes or workplace visits but also the amount of effort required to undertake, evaluate and complete all assessment requirements, including any non-classroom activities.

Pre-requisites and Co-requisites

ONPS5655C Perform Aseptic techniques

ONPS 5656C Microscopy

Course Description

This unit of competency covers the ability to prepare primary tissue cultures for applications, such as maintenance of animal cell lines and propagation of plants by tissue culture and basic subculture procedures. Personnel are required to manipulate equipment and materials and samples to prevent contamination at all preparation stages. They will have ready access to enterprise procedures and will work under direct supervision.


National Codes, Titles, Elements and Performance Criteria

National Element Code & Title:

MSL974011A Prepare tissue and cell cultures

Element:

1. Work safely according to the legal and regulatory framework

Performance Criteria:

1.1. Ensure work practices and personal actions conform to regulations, codes, guidelines and enterprise quality assurance procedures
1.2. Identify hazards and enterprise controls associated with the sample, preparation methods, reagents and equipment
1.3. Select, fit and use personal protective clothing and safety equipment
1.4. Address hazards and incidents as they arise
1.5. Maintain a chain of custody, traceable to the worker, for all cells and tissues

Element:

2.Prepare and test cell and tissue culture media

Performance Criteria:

2.1. Select and confirm media specifications and processes/methods
2.2. Prepare culture media to suit the application
2.3. Sterilise culture media and check for sterility
2.4. Perform quality control checks to ensure that culture media is fit for purpose
2.5. Store culture media in accordance with specifications

Element:

3. Prepare tissue or cell cultures

Performance Criteria:

3.1. Select tissue/cell sample to optimise growth and prepare it for culture
3.2. Add specified growth agents and/or nutrients
3.3. Inoculate culture medium using aseptic techniques

Element:

4. Monitor tissue or cell culture

Performance Criteria:

4.1. Incubate culture in specified conditions
4.2. Monitor growth of culture and record appearance and characteristics
4.3. Report presence or absence of contamination
4.4. Subculture the culture to continue the cell line
4.5. Dispose of biohazardous and other laboratory waste safely

Element:

5. Maintain records

Performance Criteria:

5.1. Maintain records of batches of media and test data
5.2. Ensure records of tissue cultures are retrievable, legible and accurate
5.3. Ensure records conform to information management, records, quality system and legal requirements


Learning Outcomes



Details of Learning Activities

  • Teacher directed delivery of lessons
  • Whole class discussion
  • Pair/group discussion and self assessment
  • Worksheets
  • Laboratory experiments
  • Record keeping of experiments
  • Research activities


 


Teaching Schedule

Week Topic/Overview Practical/Activity/PresentationAssessment12345678910a10b111213141516
Topic 1: Intro to Biohazard cabinets, aseptic technique and tissue culture

PRAC 1: Introduction to the Biohazard hood, aseptic technique and tissue culture
 

 
Topic 2: Introduction to plant tissue culture & plant physiology

PRAC 2: Seed (Lepidium sativum) propagation

PRAC 3: Media preparation
 

 
Topic 3: Plant growth

 PRAC 4: Lepidium sativum seedling transfer

PRAC 5: Cauliflower explant

 
Topic 4: Plant reproduction

PRAC 6: Surface sterilisation preparation

Collect PRAC 5 results

 
Topic 5: Tissue culture environmentPRAC 7: Surface sterilisation and explant of African Violet leaves 
Topic 6: Media & Growth RegulatorsPRAC 8: Effect of surface sterilisation and explant of Petunia leaves
& Collect results from prac 6
 
 PRAC 9: Collect and compare results from PRAC 6 and 7

Discussion of PRAC results and Assessment 1
 

 Assessment 1: Prac report 1 (PRAC 6, 7, 8 & 9)
DUE: Week 10b (30%)
 
Exam Week  
Topic 7: Micropropagation 1 PRAC 10: Carrot callus culture 
Topic 8: Micropropagation 2Library research for group presentation  
 
Student VacationPRAC 6: 
 Topic 9: ContaminationPRAC 11: Succulent explant and collect PRAC 10 results (3 weeks) 
 Topic 10 - GMOPRAC 12: Collect PRAC 10 (4 weeks) & PRAC 11 results 
Presentation prep


 

 
  RevisionActivity 4 – Group presentations
PRAC 13: Collect PRAC 11 results and take plants home
 

 Assessment 2: Group presentation:
DUE: Draft Week 11
Presentation: Week 13 (30%)
 

 Practical exam (Explant) Assessment 3: Practical Exam – Competency based assessment (C/NYC) (Technical skills)


Assessment 4: Log Book – Competency based assessment (C/NYC) (Observational and not keeping skills)
DUE Date: Week 15
 

End of Semester ExamNo PracticalAssessment 5: End of year quiz (40%)


Learning Resources

Prescribed Texts


References


Other Resources


Overview of Assessment

Asessment for this course may include tests, practical reports, assignments and presentations


Assessment Tasks

Assessment 1: Prac report (30%)

Assessment 2: Group presentation (30%)

Assessment 3: Practical Exam ( C/NYC)

Assessment 4: Log Book (C/NYC)

Assessment 5: End of year quiz (40%)


Assessment tasks will assess the student’s ability to demonstrate the following competencies:

  • OH&S requirements and code of conduct in a tissue culture laboratory
  • Ability to prepare reagents and tissue culture media accordingly
  • Culturing cells aseptically
  • Monitoring the growth of cultures and distinguishing any normal from abnormal cells as well as contamination
  • Proper record maintenance and analysis of results


Assessment Matrix

Other Information

Assessment Information

• To pass the course you need to pass, on average, each type of assessment (exam, prac reports, and assignments etc.). For example, if there are two tests you need to have an average of 50% to pass. But you also need to have passed the other assessments as well. You can’t make up marks from one type of assessment to another (eg pass the tests but fail the prac reports).

• Late work that is submitted without an application for an extension will not be corrected.

• Extension of time for submission of assessable work- A student may apply for an extension of up to 7 days from the original due date. They must lodge the application form (available on the web http://mams.rmit.edu.au/seca86tti4g4z.pdf ) at least the day before the due date. The application is lodged with the School Admin Office on Level 6, Bdg 51. Students requiring longer extensions must apply for Special Consideration (form available on the Web). For missed assessments such as exams- you (& your doctor if you are sick) must fill out a special consideration form. This form must be lodged at the HUB or online with supporting evidence (eg medical certificate), prior to, or within, 48 hours of the scheduled time of examination.

•  If you miss an assessment task due to unavoidable circumstances, you need to follow the procedure of special consideration and apply within the allowed time frame.

If you have any questions about this assessment process, feel free to email your teacher
 

Course Overview: Access Course Overview