Course Title: Apply routine chromatographic techniques
Part B: Course Detail
Teaching Period: Term2 2012
Course Code: ONPS5663C
Course Title: Apply routine chromatographic techniques
School: 155T Vocational Health and Sciences
Campus: City Campus
Program: C5282 - Diploma of Laboratory Technology (Biotechnology)
Course Contact: Amberlee Mitton
Course Contact Phone: +61 3 9925 8053
Course Contact Email: amber.mitton@rmit.edu.au
Name and Contact Details of All Other Relevant Staff
Kirsten Balding
kirsten.balding@rmit.edu.au
contact by email, or talk in class
Nominal Hours: 120
Regardless of the mode of delivery, represent a guide to the relative teaching time and student effort required to successfully achieve a particular competency/module. This may include not only scheduled classes or workplace visits but also the amount of effort required to undertake, evaluate and complete all assessment requirements, including any non-classroom activities.
Pre-requisites and Co-requisites
MSL974003A Perform chemical tests and procedures
MSL973007A Perform microscopic examination
MSL974006A Perform biological procedures
MSL974001A Prepare, standardise and use solutions
Course Description
This unit of competency covers the ability to analyse samples using routine chromatographic techniques.
The unit also includes establishing client needs for routine and non-routine samples, optimising enterprise procedures and instruments for specific samples, obtaining valid and reliable data and reporting test results.
Personnel are required to recognise atypical test data/results and troubleshoot common analytical procedure and equipment problems.
National Codes, Titles, Elements and Performance Criteria
National Element Code & Title: |
MSL975009A Apply routine chromatographic techniques |
Element: |
1. Establish client needs and schedule analysis |
Performance Criteria: |
1.1. Liaise with client or sample provider to determine client needs and sample history |
Element: |
2. Prepare samples and standards |
Performance Criteria: |
2.1. Obtain a representative analytical portion of the laboratory sample |
Element: |
3. Set up and optimise instrument |
Performance Criteria: |
3.1. Perform pre-use and safety checks in accordance with enterprise procedures |
Element: |
4. Perform analysis |
Performance Criteria: |
4.1. Measure analyte response for standards, validation checks and samples |
Element: |
5. Process and analyse data |
Performance Criteria: |
5.1. Confirm data is the result of valid measurements |
Element: |
6. Maintain a safe work environment |
Performance Criteria: |
6.1. Identify risks, hazards, safety equipment and control measures associated with sample handling, preparation and analytical method |
Element: |
7. Maintain laboratory records |
Performance Criteria: |
7.1. Enter approved data and results into laboratory information management system (LIMS) |
Learning Outcomes
Details of Learning Activities
Learning activities for this course will include:
• Teacher directed face-to face delivery of lessons
• Whole class Discussion
• Pair/group discussion
• Small group workshops
• Online quizzes & theory activities
• Worksheets including Revision
• Laboratory experiments
• Record keeping of experiments
• Presentations
• Research activities
See the Teaching Schedule (below) for details.
Teaching Schedule
Date (Mon) | Week | Theory (online) | Prac (4 hr) | Assessment |
9/7 |
1 | * Course Overview | Safety in the Chromatography Lab Making solutions |
Safety checks |
|
* Paper Chromatography Theory - concepts & calcs | |||
16/7 |
2 | Paper Chromatography Theory | Paper Chromatography of food dyes | |
23/7 |
3 | TLC Theory concepts & calcs | Thin Layer Chromatography of Vegetable pigments | Group 1 oral prac report* Week 3 Quiz |
30/7 |
4 | Gel Chromatography (Size Exclusion Chromatography (SEC)) Theory | SEC Chromatography | Group 2 oral prac report* |
6/8 |
5 | Ion Exchange (IE) Chromatography Theory | Ion Exchange Chromatography | Group 3 oral prac report* Week 5 Quiz |
13/8 |
6 | Affinity chromatography & Multistep chromatography - concepts Case study: CSL |
Multistep chromatography - Rainbow chromatography: isolating a mixture of food dyes | Group 4 oral prac report* Week 6 Quiz - CSL case study |
20/8 |
7 | Revision | no prac class | |
Week of 27/8 | 8 | Exam Week | Test 1 (theory) | Test 1 |
3/9 |
9 | HPLC theory & calculations - peak area, retention time, internal standards Solid Phase Extraction (SPE) |
Solution Making for HPLC GFP &/or BFP from E. coli cells. |
Week 9 Quiz |
10/9 |
10 | HPLC - isocratic v’s gradient chromatography theory | 1. HPLC of caffeine in drinks | Group 5 oral prac report* Prac Report 2 - caffeine in drinks (experiment) |
17/9 | 11 | HPLC - isocratic v’s gradient chromatography theory | 1. HPLC of caffeine in drinks - interpreting results |
Prac Report 2 - caffeine in drinks (analysis) |
Week of 24/9 |
Student vacation |
|||
1/10 |
12 | Example calcs for analgesics HPLC prac, interpreting results, practising calcs & answering Q’s | HPLC of Analgesics (Painkiller) Tablets TLC of aspirin, caffeine & paracetamol |
Week 12 Quiz |
8/10 | 13 | Example calcs for analgesics HPLC prac, interpreting results, practising calcs & answering Q’s | HPLC of Analgesics (Painkiller) Tablets interpreting results | Submit Logbook |
15/10 |
14 | GC theory & calculations Internal standards |
GC @ Applied Chemistry Gas Chromatography of Xylene (to be confirmed) |
Week 14 Quiz |
22/10 | 15 | Revision | ||
Week of 29/10 |
16 | Exam Week | Test 2 | Test 2 |
Week of 5/11 |
17 | Alternative assessment (special consideration only) | ||
* Oral practical report = powerpoint presentation to class |
Learning Resources
Prescribed Texts
Perform Routine Chromatographic Techniques Practical Manual (available online) |
|
• J Crowe, T. Bradshaw, P. Monk (2006) |
References
Other Resources
Overview of Assessment
Assessment for this course may include laboratory reports, assignments, exams and practival assessment in the laboratory
Assessment Tasks
1. Safety Checks
Week done: 1
Due: in class
%: satisfactory (ungraded)
2. Prac Report 1 - Oral group report (powerpoint presentation to class)
Week done: One group per week only at start of class
Topic: for experiments done during weeks 2, 3, 4, 5, and 6
Week due: Talk to be done 2 weeks after the experiment
%: 15%
3. Quizzes
Week done: 3,5,6,9,12,14
Topic: see lab manual ’assessment guide’ section
Week due: various - see blackboard
%: 20 (calculated from average grade)
4. Prac Report 2 (spreadsheet assignment)
Week done: 10/11
Topic: HPLC of caffeine in drinks
Week due: 11 (in class activity)
%: 15%
5. Exam 1
Week done: 8
Topic: Chromatography (Paper, TLC, SEC, IEC, multistep)
n/a
%: 25%
6. Exam 2
Week done: 16
Topic: HPLC & GC only
Week due: n/a
%: 25%
7. Logbook
Week done: all
Requirement: Logbook - signed off if considered competent** by teacher & recorded on central record/ Sign off each week lab class is attended**
Week due: Submit completed logbook at end of week 13 (monday week 14 is last day to avoid penalty to grades)
%: satisfactory (ungraded)
8. Attendance
Week done: all
Requirement: 80% attendance or more is required
Week due: n/a
%: satisfactory
Total 100%
Assessment Matrix
Other Information
*The topic of the presentation is 2 weeks before the talk. For example, TLC of vegetable pigments is done in week 3 lab class, the talk on this topic will be held in week 5. The presentation should contain a brief explanation of how the separation works, results and a short discussion of trends in the data and what the results tell you about the mixture being separated. Talks should be no more than 10 minutes long. All group members must present part of the talk.
** see logbook information for what is required.
IMPORTANT POINTS TO NOTE
· If you miss a prac report class you will only be eligible to write up/present a different prac if you provide a medical certificate. For this to occur you need to discuss your situation with the teacher as soon as possible. Other reasons for missing a write up prac do not mean that you can write up a different report. If you miss an oral report presentation day you will be able to present it at the next class you attend as long as you provide a medical certificate.
IMPORTANT POINTS TO NOTE ABOUT ASSESSMENT CONTINUED…
· If you miss the experiment that you are to present as an oral presentation, you may present a different experiment as long as you provide a medical certificate
· Practical report 2 (a spreadsheet activity) is due in the analysis session.
· Each quiz has a published due date. Quizzes are to be done before the class in the same week to help you prepare for the class. Quizzes may include questions on safety.
· To pass the quizzes component you must get an average pass for all quizzes (ie. 50% average). The score for quizzes is calculated from the average grade of all quizzes. If you miss a quiz and have submitted a medical certificate you may do it later than the due date as long as you contact the teacher asap after your absence.
· Students must attend at least 80 % of practical classes to pass the course.
· Students must pass all of the assessment tasks to pass the course.
· Reports should be submitted online via blackboard.
· Please read the online anti-plagiarism statement given in blackboard submission of work via Blackboard incorporates student agreement to these conditions.
Course Overview: Access Course Overview