Course Title: Apply routine chromatographic techniques

Part B: Course Detail

Teaching Period: Term2 2015

Course Code: ONPS5663C

Course Title: Apply routine chromatographic techniques

School: 155T Vocational Health and Sciences

Campus: City Campus

Program: C5282 - Diploma of Laboratory Technology (Biotechnology)

Course Contact: Amberlee Mitton

Course Contact Phone: +61 3 9925 8053

Course Contact Email:

Name and Contact Details of All Other Relevant Staff

Kirsten Balding

contact by email, or talk in class

Nominal Hours: 120

Regardless of the mode of delivery, represent a guide to the relative teaching time and student effort required to successfully achieve a particular competency/module. This may include not only scheduled classes or workplace visits but also the amount of effort required to undertake, evaluate and complete all assessment requirements, including any non-classroom activities.

Pre-requisites and Co-requisites

MSL974003A Perform chemical tests and procedures
MSL973007A Perform microscopic examination
MSL974006A Perform biological procedures
MSL974001A Prepare, standardise and use solutions

Course Description

This unit of competency covers the ability to analyse samples using routine chromatographic techniques.
The unit also includes establishing client needs for routine and non-routine samples, optimising enterprise procedures and instruments for specific samples, obtaining valid and reliable data and reporting test results.
Personnel are required to recognise atypical test data/results and troubleshoot common analytical procedure and equipment problems.

National Codes, Titles, Elements and Performance Criteria

National Element Code & Title:

MSL975009A Apply routine chromatographic techniques


1. Establish client needs and schedule analysis

Performance Criteria:

1.1. Liaise with client or sample provider to determine client needs and sample history
1.2. Record sample description, compare with specification and record and report discrepancies
1.3. Identify non-routine samples and the possible need to vary enterprise procedures
1.4. Seek advice from supervisor about any proposed variations and document all approved changes
1.5. Schedule analysis using enterprise procedures


2. Prepare samples and standards

Performance Criteria:

2.1. Obtain a representative analytical portion of the laboratory sample
2.2. Prepare sample in accordance with testing requirements
2.3. Prepare validation checks for analytical portion


3. Set up and optimise instrument

Performance Criteria:

3.1. Perform pre-use and safety checks in accordance with enterprise procedures
3.2. Start up and condition the instrument using enterprise procedures
3.3. Optimise instrumental parameters to suit sample and test requirements
3.4. Check calibration status of instrument and perform calibration using specified standards and procedures, if applicable


4. Perform analysis

Performance Criteria:

4.1. Measure analyte response for standards, validation checks and samples
4.2. Conduct sufficient measurements to obtain reliable data
4.3. Return instruments to standby or shutdown condition, as required


5. Process and analyse data

Performance Criteria:

5.1. Confirm data is the result of valid measurements
5.2. Perform required calculations and ensure results are consistent with standards or estimations and expectations
5.3. Record results with the appropriate accuracy, precision and units
5.4. Analyse trends in data and/or results and report out of specification or atypical results promptly to appropriate personnel
5.5. Troubleshoot analytical procedure or equipment problems which have led to atypical data or results


6. Maintain a safe work environment

Performance Criteria:

6.1. Identify risks, hazards, safety equipment and control measures associated with sample handling, preparation and analytical method
6.2. Use personal protective equipment and safety procedures specified for test method and materials to be tested
6.3. Minimise the generation of wastes and environmental impacts
6.4. Ensure the safe disposal of laboratory wastes
6.5. Clean, care for and store equipment and consumables in accordance with enterprise procedures


7. Maintain laboratory records

Performance Criteria:

7.1. Enter approved data and results into laboratory information management system (LIMS)
7.2. Maintain equipment logs in accordance with enterprise procedures
7.3. Maintain security and confidentiality of laboratory data and enterprise information
7.4. Communicate results to appropriate personnel

Learning Outcomes

Details of Learning Activities

Learning activities for this course will include:
• Teacher directed face-to face delivery of lessons
• Whole class Discussion
• Pair/group discussion
• Small group workshops
• Online quizzes & theory activities
•  Worksheets including Revision
• Laboratory experiments
• Record keeping of experiments
• Presentations
• Research activities

Teaching Schedule

Date (Mon)WeekTheory (online)Lab (4 hr)Assessment
6/71* Course OverviewSafety in the Chromatography Lab
Making solutions


 * Paper Chromatography Theory - concepts & calcs  
13/7 2 Paper Chromatography Theory Paper Chromatography of food dyes Logbook
20/7 3 TLC Theory concepts & calcsThin Layer Chromatography of Vegetable pigments 

Quiz 1
27/7 4 Gel Chromatography (Size Exclusion Chromatography (SEC)) Theory SEC ChromatographyGroup 1 oral lab report*
3/85 Ion Exchange (IE) Chromatography TheoryIon Exchange Chromatography

Quiz  2

Group 2 oral lab report*

10/86 Affinity chromatography (AC) & Multistep chromatography - concepts

  Case study: CSL 

Multistep chromatography
CSL case study

no lab class


Quiz 3
CSL case study

Group 3 oral lab report*

17/8 7 Revisionno lab class 
Week of 25/8 8 Exam Week Exam 1 (theory)  Exam 1
1/9  Student vacation  
8/9 9 HPLC theory & calculations - peak area, retention time, internal standards

Solid Phase Extraction (SPE)
Solution Making for HPLC
SEC extraction of GFP &/or BFP from E. coli cells.

Quiz 4

Group 4 oral lab report*

15/9 10 HPLC - isocratic v’s gradient chromatography theoryHPLC of caffeine in drinksGroup 5 oral lab report*

Lab Report 2 - caffeine in drinks (experiment)
22/911  HPLC - isocratic v’s gradient chromatography theory HPLC of caffeine in drinks -
interpreting results
Lab Report 2 - caffeine in drinks (analysis)
29/9 12 Example calcs for analgesics HPLC prac,  interpreting results, practising calcs & answering Q’s  HPLC of Analgesics  (Painkiller) Tablets

TLC of aspirin, caffeine & paracetamol
Quiz 5
6/10 13 Example calcs for analgesics HPLC prac,  interpreting results, practising calcs & answering Q’s HPLC of Analgesics  (Painkiller) Tablets  interpreting results Submit Logbook
13/1014 GC theory & calculations
Internal standards
 GC @ Applied Chemistry
Gas Chromatography of Xylene
(to be confirmed)
Quiz 6
 20/10 15 Revision  
Week of 27/10
 16 Exam Week Exam 2Exam 2
 Week of 3/11
 17 Alternative assessment (special consideration only)  

 * Oral lab report
= powerpoint presentation to class, experiment done 2 weeks earlier

**Logbook = Logbook and Labskills Checklist


Learning Resources

Prescribed Texts

Perform Routine Chromatographic Techniques Practical Manual (available online)

• J Crowe, T. Bradshaw, P. Monk (2006)
Chemistry for the Biosciences. The essential concepts.
pp 270-274 Oxford University Press, Oxford.

Available from the course Blackboard or directly from the RMIT Library’s eReserve collection & is available after login from the following link:

Also available in Carlton Library as Hardcopy : Call No. 540.078 C953


Other Resources

Overview of Assessment

Assessment for this course may include laboratory reports, assignments, exams and practival assessment in the laboratory

Assessment Tasks

Assessment 1.
Online Theory  Quizzes
Week 3: Quiz 1: TLC, Paper chroma
Week 5: Quiz 2: SEC, IEC
Week 6: Quiz 3: CSL Case study
Week 9: Quiz 4: AC, HIC, SPE
Week 12: Quiz 5: HPLC
Week 14: Quiz 6: GC Experiment

Must have an average pass grade for the best 5 quizzes (i.e. one quiz can be missed without affecting the grade). Quizzes must be submitted by the due date (shown in Blackboard).
Value: 20%

Assessment 2.
2 Theory Exams
Exam 1 Topics: Paper Chromatography, TLC, SEC, IEC, and multistep Chromatography
Exam 2 Topics: HPLC & GC only
End of terms 3 and 4
Value: 25%+ 25% = 50%
Must pass exams on average

Assessment 3. Laboratory practice.
Value: Satisfactory completion required

a. 2 Prac Reports
1: Oral group report* (powerpoint presentation to class)
2: HPLC of Caffeine in drinks spreadsheet assignment

Report 1:
When: 1 group per week only for experiments done during weeks 2, 3, 4, 5.
Talk done 2 week’s later at start of class

Report 2:
When: Complete during class weeks 10 & 11

Value: 15%+ 15% = 30%
Must pass reports on average

b. Logbook and Labskills:
Each week’s entry is to be signed off by student and teacher when it is considered ‘satisfactory’** and complete. Students must write up their logbook for at least 80% of labs.
Only labs where the student was present may be written up.

When: All lab sessions attended
Due: Submit completed logbook checklist by Monday 5pm of week 14
Value: Satisfactory completion required
Total 100%

*The topic of the presentation is 2 weeks before the talk. For example, TLC of vegetable pigments is done in week 3 lab class, the talk on this topic will be held in week 5. The presentation should contain a brief explanation of how the separation works, results and a short discussion of trends in the data and what the results tell you about the mixture being separated. Talks should be no more than 10 minutes long. All group members must present part of the talk.
** see logbook information for what is required.

Oral Practical Reports

• If you miss the experiment that you are to present as an oral presentation, you may present a different experiment as long as you provide a medical certificate
Practical Reports
• If you miss a prac report class you will only be eligible to write up/present a different prac if you provide a medical certificate. For this to occur you need to discuss your situation with the teacher as soon as possible. Other reasons for missing a write up prac do not mean that you can write up a different report. If you miss an oral report presentation day you will be able to present it at the next class you attend as long as you provide a medical certificate.
• Practical report 2 (a spreadsheet activity) is due in the analysis session.

• Each quiz has a published due date. Quizzes are to be done before the class in the same week to help you prepare for the class. 
• To pass the quizzes component you must get an average pass for all quizzes (ie. 50% average). The score for quizzes is calculated from the average grade of all quizzes. If you miss a quiz and have submitted a medical certificate you may do it later than the due date as long as you contact the teacher asap after your absence.
• If you miss a prac class you should provide a copy of your medical certificate or other evidence (court appearance, funeral notice, statutory declaration) to the teacher.


Assessment Matrix

Other Information

This course is graded in accordance with competency-based assessment, but which also utilise graded assessment
CHD Competent with High Distinction (80 – 100%)
CDI Competent with Distinction (70 – 79%)
CC Competent with Credit (60 – 69%)
CAG Competency Achieved – Graded (50 – 59%)
NYC Not Yet Competent
DNS Did Not Submit for assessment

• To pass the course you need to pass, on average, each type of assessment (exams, pracs and assignments etc.) For example, if there are two exams you need to have an average of 50% to pass and you also need to have passed the other assessment types. You can’t make up marks from one type of assessment to another (e.g. pass the exams but fail the prac component).

• Late work that is submitted without an application for an extension (see below) will not be corrected.

• APPLICATION FOR EXTENSION OF TIME FOR SUBMISSION OF ASSESSABLE WORK - A student may apply for an extension of up to 7 days from the original due date. They must lodge the application form (available online at least 24 hours before the due date. The application is lodged with the School Admin Office on Level 6, Bdg 51. Students requiring longer extensions must apply for SPECIAL CONSIDERATION.

• For missed assessments such as exams- you (& your doctor if you are sick) must fill out a special consideration form. This form must be lodged online with supporting evidence prior to, or within, 48 hours of the scheduled time of examination

Course Overview: Access Course Overview