Course Title: Apply electrophoretic techniques

Part B: Course Detail

Teaching Period: Term1 2017

Course Code: BIOL5232C

Course Title: Apply electrophoretic techniques

School: 174T School of VE Engineering, Health & Science

Campus: City Campus

Program: C5363 - Diploma of Laboratory Technology (Biotechnology)

Course Contact: Amber Mitton

Course Contact Phone: +61 3 9925 8053

Course Contact Email:

Name and Contact Details of All Other Relevant Staff

Kirsten Balding

Phone: 9925 4283 (office hours)

Nominal Hours: 100

Regardless of the mode of delivery, represent a guide to the relative teaching time and student effort required to successfully achieve a particular competency/module. This may include not only scheduled classes or workplace visits but also the amount of effort required to undertake, evaluate and complete all assessment requirements, including any non-classroom activities.

Pre-requisites and Co-requisites



MSL974006 Perform biological procedures


MSL974003 Perform chemical tests and procedures

Course Description


This unit of competency covers the ability to analyse samples using electrophoretic techniques. The unit also includes establishing client needs for routine and non-routine samples, optimising workplace procedures and instruments for specific samples, obtaining valid and reliable data and reporting test results. Personnel are required to recognise atypical test data/results and troubleshoot common analytical procedure and equipment problems. 

National Codes, Titles, Elements and Performance Criteria

National Element Code & Title:

MSL975008 Apply electrophoretic techniques


1 Establish client needs and schedule analysis

Performance Criteria:

1.1 Liaise with client or sample provider to determine client needs and sample history

1.2 Record sample description, compare with specification and record and report discrepancies

1.3 Identify non-routine samples and the possible need to vary workplace procedures

1.4 Seek advice from supervisor about any proposed variations and document all approved changes

1.5 Schedule analysis using workplace procedures


2 Prepare samples and standards

Performance Criteria:

2.1 Obtain a representative analytical portion of the laboratory sample

2.2 Prepare sample in accordance with testing requirements

2.3 Prepare validation checks for analytical portion


3 Set up and optimise instrument

Performance Criteria:

3.1 Perform pre-use and safety checks in accordance with workplace procedures

3.2 Start up and condition the instrument using workplace procedures

3.3 Optimise instrumental parameters to suit sample and test requirements

3.4 Check calibration status of instrument and perform calibration using specified standards and procedures, as required


4 Perform analysis

Performance Criteria:

4.1 Measure analyte response for standards, validation checks and samples

4.2 Conduct sufficient measurements to obtain reliable data

4.3 Return instruments to standby or shutdown condition as required


5 Process and analyse data

Performance Criteria:

5.1 Confirm data is the result of valid measurements

5.2 Perform required calculations and ensure results are consistent with standards or estimations and expectations

5.3 Record results with the appropriate accuracy, precision and units

5.4 Analyse trends in data and/or results and report out-of-specification or atypical results promptly to appropriate personnel

5.5 Troubleshoot analytical procedure or equipment problems which have led to atypical data or results


6 Maintain a safe work environment

Performance Criteria:

6.1 Identify risks, hazards, safety equipment and control measures associated with sample handling, preparation and analytical method

6.2 Use personal protective equipment (PPE) and safety procedures specified for test method and materials to be tested

6.3 Minimise the generation of waste and environmental impacts

6.4 Ensure the safe disposal of laboratory waste

6.5 Clean, care for and store equipment and consumables in accordance with workplace procedures


7 Maintain laboratory records

Performance Criteria:

7.1 Enter approved data and results into laboratory information management system (LIMS)

7.2 Maintain equipment logs in accordance with workplace procedures

7.3 Maintain security, integrity and traceability of samples and documentation

7.4 Communicate results to appropriate personnel

Learning Outcomes

Details of Learning Activities

Lab classes and Record keeping
Reports and Assignments
Online theory and quizzes

Teaching Schedule


Learning Resources

Prescribed Texts

Lab Manual - Term 1 (DNA)
Lab Manual - Term 2 (Protein)
Both Lab manuals are available from the course Blackboard. Please print the manual in time for the week 1 class. Print the whole manual at the start of the semester - bring it to class each week so you have the instructions for each experiment. The Term 2 Manual will be available towards the beginning of term 2.

Resources available on Blackboard for this course


Other Resources

Overview of Assessment

online quizzes

written tests

practical reports

laboratory logbook

Assessment Tasks


Assessment 1. Theory
a. Weekly Quizzes 10% (best 10 grades count @ 2% each) There is a quiz most weeks. Due on Blackboard.
b. 2 Theory Exams 25% + 25% = 50% Week of 4/4/16 and Week of 30/5/16

Assessment 2. Prac Reports
1: Agarose Electrophoresis of DNA 15%
Lab: 24/2/16 & 2/3/16 (choose lab 3 or 4)

2. 2: SDS-PAGE of Albumin 15%
Lab 8:  27/4/16

Assessment 3. Logbook and Lab Skills Record
10% logbook and satisfactory Labskills (satisfactory records kept/participation for at least 80% of lab classes)
Use the self assessment checklist to see if you have completed the record, then get it assessed within 2 weeks of the class. To be considered satisfactory each record will be assessed against the checklist and signed off by the supervisor on the central logbook checklist sheet.
There will be a mid-semester review of logbook and lab skills followed by a final assessment (16/5/16)

Total     100%        

Assessment Matrix

Other Information

Assessment Information

• This course is graded in accordance with competency-based assessment, but which also utilise graded assessment
CHD Competent with High Distinction (80 – 100%)
CDI Competent with Distinction (70 – 79%)
CC Competent with Credit (60 – 69%)
CAG Competency Achieved – Graded (50-59%)
NYC Not Yet Competent (0 – 49%)
DNS Did Not Submit for assessment

• To pass the course you need to pass, on average, each type of assessment (exams, pracs, and assignments etc.) For example, if there are two exams you need to have an average of 50% to pass and you also need to have passed the other assessment types. You can’t make up marks from one type of assessment to another (e.g. pass the exams but fail the prac component).

• Late work that is submitted without an application for an extension (see below) will not be corrected.

• APPLICATION FOR EXTENSION OF TIME FOR SUBMISSION OF ASSESSABLE WORK - A student may apply for an extension of up to 7 days from the original due date. They must lodge the application form (available online at least 24 hours before the due date. The application is lodged with the School Admin Office on Level 6, Bdg 51. Students requiring longer extensions must apply for SPECIAL CONSIDERATION.

• For missed assessments such as exams- you (& your doctor if you are sick) must fill out a special consideration form. This form must be lodged online with supporting evidence prior to, or within, 48 hours of the scheduled time of examination


  • A minimum of 80% attendance at practical classes is required to pass this course. If you are sick you should submit a medical certificate to 6th floor office addressed to the Program Coordinator. If you miss a practical class that is a prac report write up you need to provide a medical certificate (or other evidence) and arrange an alternative report with your teacher



Plagiarism is the presentation of the work, idea or creation of another person as though it is your own. It is a form of cheating and is a very serious academic offence that may lead to expulsion from the University. Plagiarised material can be drawn from, and presented in, written, graphic and visual form, including electronic data and oral presentation. Plagiarism occurs when the origin of the material used is not appropriately cited. It also occurs through enabling plagiarism, which is the act of assisting or allowing another person to plagiarise or to copy your own work. Please make sure you consider this carefully in completing all your work and assessments in this course and if you are unsure about whether you might have plagiarised, seek help from your teacher. 


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