Course Title: Apply routine chromatographic techniques

Part B: Course Detail

Teaching Period: Term2 2020

Course Code: BIOL5256C

Course Title: Apply routine chromatographic techniques

School: 174T School of VE Engineering, Health & Science

Campus: City Campus

Program: C5388 - Diploma of Laboratory Technology

Course Contact: Kirsten Balding

Course Contact Phone: +61 3 9925 4283

Course Contact Email:

Name and Contact Details of All Other Relevant Staff

Kirsten Balding


Nominal Hours: 120

Regardless of the mode of delivery, represent a guide to the relative teaching time and student effort required to successfully achieve a particular competency/module. This may include not only scheduled classes or workplace visits but also the amount of effort required to undertake, evaluate and complete all assessment requirements, including any non-classroom activities.

Pre-requisites and Co-requisites

MSL974019 Perform chemical tests and procedures

Course Description

This unit of competency describes the skills and knowledge to analyse samples using routine chromatographic techniques. The unit also includes establishing client needs for routine and non-routine samples, optimising workplace procedures and instruments for specific samples, obtaining valid and reliable data and reporting test results. Personnel are required to recognise atypical test data/results and troubleshoot common analytical procedure and equipment problems.

National Codes, Titles, Elements and Performance Criteria

National Element Code & Title:

MSL975040 Apply routine chromatographic techniques


1. Establish client needs and schedule analysis

Performance Criteria:

1.1 Record sample description, compare with specification and record and report any discrepancies 1.2 Identify non-routine samples and the possible need to vary workplace procedures 1.3 Seek advice from supervisor about any proposed variations and document all approved changes 1.4 Schedule analysis using workplace procedures


2. Prepare samples and standards

Performance Criteria:

2.1 Obtain a representative analytical portion of the laboratory sample 2.2 Prepare sample in accordance with testing requirements 2.3 Prepare validation checks for analytical portion


3.Set up and optimise instrument

Performance Criteria:

3.1 Perform pre-use and safety checks in accordance with workplace procedures 3.2 Start up and condition the instrument using workplace procedures 3.3 Optimise instrumental parameters to suit sample and test requirements 3.4 Check calibration status of instrument and perform calibration using specified standards and procedures, as required 3.5 Clean, care for and store equipment and consumables in accordance with workplace procedures


4. Perform analysis

Performance Criteria:

4.1 Measure analyte response for standards, validation checks and samples 4.2 Conduct sufficient measurements to obtain reliable data 4.3 Return instruments to standby or shutdown condition, as required


5.Process and analyse data

Performance Criteria:

5.1 Confirm data is the result of valid measurements 5.2 Perform required calculations and ensure results are consistent with standards or estimations and expectations 5.3 Record results with the appropriate accuracy, precision and units 5.4 Analyse trends in data and/or results and report out-of-specification or atypical results promptly to appropriate personnel 5.5 Troubleshoot analytical procedure or equipment problems which have led to atypical data or results


6. Maintain laboratory records

Performance Criteria:

6.1 Count cells in samples 6.2 Prepare serial dilutions of samples aseptically for culture and colony counting 6.3 Indicate the appropriate dilution necessary to reliably count organisms in a serially diluted sample 6.4 Count colonies for calculating number of viable organisms per unit volume

Learning Outcomes

Details of Learning Activities

practical classes

Online theory 

group work


online activities


Teaching Schedule


Note that the program has been altered due to COVID19 restrictions.

WeekDate2020 ProgramAssessment
16-JulPaper Chromatography Theory
Lab 1: Online
1.Course Overview
2.Safety in the Lab
3. Making solutions (calcs), start Paper Chromatography of food dyes analysis
AT5: Lab Records
213-JulLab 2: Online
Paper Chromatography of food dyes Report - in class
AT5: Lab Records
AT1: Paper chromatography report
320-JulTLC Theory
Lab 3: Online
Thin Layer Chromatography of Vegetable pigments

AT5: Lab Records
AT3: KE Questions (Test 1) Available

AT2: Quiz

427-JulLab 4: Online
Thin Layer Chromatography Tablets
AT5: Lab Records
53-AugComplete Logbook AssessmentsAT5: Lab Records
610-AugColumn Chromatography Theory: Size Exclusion (SEC) Ion Exchange (IEC); Affinity chromatography (AC)

AT3: KE questions (Test 1)

AT2: Quiz

717-AugFinalise logbook & KE questionsAT3: KE questions (Test 1)
824-Aug"HPLC theory & calculations - peak area, retention time, Using standards to find the concentration of a substance"
Lab 7: Online
HPLC of caffeine in drinks
Case studies – Common problems and Troubleshooting
AT4: HPLC Report/
AT6: KE Questions Available (Test 2)
 31-AugMid- semester break 
97-SepTheory: HPLC - isocratic v's gradient chromatography; Qualitative and Quantitative HPLC – Using standards to find what substances are in a sample and at what concentration
Lab 8:
HPLC of Tablets;

AT6: KE Questions (Test 2)

AT2: Quiz

1014-SepFinalise KE questions (HPLC)AT6: Finalise KE questions (Test 2)
1121-SepLABSKILLS 1: paper chroma 1 (single conc, do individually) (SOP adjustments: non-routine samples, Sample prep: dilution)
(sample 1 - dye)
AT5: Labskills
1228-SepLABSKILLS 1: paper chroma 1 (single conc, do individually) (SOP adjustments: non-routine samples, Sample prep: dilution)
(sample 2 - other dye)

AT5: Labskills

AT2: Quiz

Thin Layer Chromatography of Vegetable pigments (Spinach+ red Lettuce)
Needs Rf values for validity
(Method optimisation & effect of the solvent) (sample prep: solvent extraction & grinding) (sample 2 vegetables)
AT5: Labskills
1412-OctLABSKILLS 2:
Thin Layer Chromatography of more Vegetable pigments (Spinach & red lettuce)
Needs Rf values for validity (sample prep: solvent extraction & grinding) (sample 2 vegetables)
AT5: Labskills
1519-OctLABSKILLS 3: TLC of Tablets - (Sample prep: grinding, filtering)(sample tablet 1)AT5: Labskills
1626-OctLABSKILLS 3: Repeat TLC of Tablets - (Sample prep: grinding, filtering)(sample tablet 2)AT5: Labskills/ Finalise all Labskills assessment records
17 alternative assessment (special consideration only) 


Learning Resources

Prescribed Texts


Other Resources

Recommended: Boyer R (2006)  Biochemistry Laboratory Modern Theory and Techniques, Benjamin Cummings Call No. 572.078 B791 

The text has good information about both Electrophoresis and Chromatography Techniques. There are a number of copies on the library that you can borrow.

Download your practical manual from Canvas and PRINT a copy.


Overview of Assessment

Assessment may include
observation of laboratory tasks
folio of laboratory results
online quizzes
analysis of experimental results

Assessment Tasks

AT1: Practical Report - Paper Chromatography

AT2: Knowledge Evidence Quizzes

AT3: Knowledge Evidence Test 1

AT4: Prac report- HPLC Report

AT5: Lab Records & Skills

Due dates are shown in Canvas. Weeks that each assessment is on is shown in the teaching schedule above.

AT6: Knowledge Evidence Test 2

Assessment Matrix

Other Information

This course is graded in accordance with competency-based assessment

CA Competency Achieved  
NYC Not Yet Competent
DNS Did Not Submit for assessment

To pass the course you need achieve a satisfactory result for all assessments. Students may be given additional opportunities to demonstrate competence. 

Late work that is submitted without an application for an extension (see below) will not be corrected.

APPLICATION FOR EXTENSION OF TIME FOR SUBMISSION OF ASSESSABLE WORK - A student may apply for an extension of up to 7 days from the original due date. They must lodge the application form ( at least 24 hours before the due date. The application should be emailed to the Program Coordinator. Students requiring longer extensions must apply for SPECIAL CONSIDERATION.

• For missed assessments such as tests- you (& your doctor if you are sick) must fill out a special consideration form. This form must be lodged online with supporting evidence prior to, or within, 5 days of the scheduled time of the assessment

Plagiarism is the presentation of the work, idea or creation of another person as though it is your own. It is a form of cheating and is a very serious academic offence that may lead to expulsion from the University. Plagiarised material can be drawn from, and presented in, written, graphic and visual form, including electronic data and oral presentation. Plagiarism occurs when the origin of the material used is not appropriately cited. It also occurs through enabling plagiarism, which is the act of assisting or allowing another person to plagiarise or to copy your own work. Please make sure you consider this carefully in completing all your work and assessments in this course and if you are unsure about whether you might have plagiarised, seek help from your teacher.

Course Overview: Access Course Overview