Course Title: Perform biological procedures
Part B: Course Detail
Teaching Period: Term2 2013
Course Code: ONPS5659C
Course Title: Perform biological procedures
School: 155T Life & Physical Sciences
Campus: City Campus
Program: C5283 - Diploma of Laboratory Technology (Pathology Testing)
Course Contact : Amberlee Mitton
Course Contact Phone: +61 3 9925 8053
Course Contact Email:amberlee.mitton@rmit.edu.au
Name and Contact Details of All Other Relevant Staff
Amberlee Mitton
Nominal Hours: 130
Regardless of the mode of delivery, represent a guide to the relative teaching time and student effort required to successfully achieve a particular competency/module. This may include not only scheduled classes or workplace visits but also the amount of effort required to undertake, evaluate and complete all assessment requirements, including any non-classroom activities.
Pre-requisites and Co-requisites
Pre-Requisites
MSL973004A - Perform aseptic techniques AND MSL973007A - Perform microscopic examination
Course Description
This unit of competency covers the ability to interpret work requirements, prepare samples, conduct pre-use and calibration checks on equipment and perform routine biological procedures, including sample preparation. These procedures may involve several steps and are used to classify cell types, species and biologically active compounds by analysing their biological and chemical characteristics. This unit includes data processing, interpretation of results and troubleshooting obvious departures from standard procedures.
National Codes, Titles, Elements and Performance Criteria
National Element Code & Title: |
MSL974006A Perform biological procedures |
Element: |
1. Interpret and schedule work requirements |
Performance Criteria: |
1.1. Review work request to identify samples, required procedures and materials/equipment/instruments involved |
Element: |
2. Receive and prepare biological samples |
Performance Criteria: |
2.1. Log samples using standard operating procedures (SOPs) |
Element: |
3. Perform techniques that assist in the classification of a cell or species |
Performance Criteria: |
3.1. Select suitable techniques in accordance with enterprise requirements and methods |
Element: |
4. Perform techniques that analyse biological activity |
Performance Criteria: |
4.1. Select suitable techniques in accordance with enterprise requirements and methods |
Element: |
5. Process and interpret data |
Performance Criteria: |
5.1. Record test data noting atypical observations |
Element: |
6. Maintain a safe work environment |
Performance Criteria: |
6.1. Use established safe work practices and personal protective equipment to ensure personal safety and that of other laboratory personnel |
Element: |
7. Maintain laboratory records |
Performance Criteria: |
7.1. Record approved data into enterprise system |
Learning Outcomes
Details of Learning Activities
- Teacher directed learning
- Worksheets
- Group work
- Practical laboratory sessions
- Student directed discussion
- Analysis of data
- Research based learning
Teaching Schedule
Week | Topic | Practical | Assessment |
1 | TOPIC 1: Intro to biomolecules and the biochemistry laboratory | Preparing solutions-Biuret & Saturated SAS Risk assessments |
Logbook |
2 | TOPIC 2: Structure and function: Proteins | Spectrophotometric Protein Determination Biuret Method | Logbook |
3 | TOPIC 3: Structure and function: Enzymes | Rapid Biuret Protein Determination | Logbook |
4 | TOPIC 4: Structure and function: Carbohydrates | Precipitation of Protein & Rapid Biuret Protein Determination |
Assessment 1: Practical Report (Precipitation of protein) Logbook |
5 | TOPIC 5: Structure and function: Lipids | Carbohydrates & Rapid Biuret Protein Determination |
Logbook |
6 | TOPIC 6: Structure and function: Nucleic acids | Fractionation of Milk & Rapid Biuret Protein Determination |
Logbook |
7 | Revision | Rapid Biuret Protein Determination & Review of Biuret Data |
Assessment 2: Practical Report (Biuret Week 2-7) 12.5% DUE: Week 10 |
8 | Mid-Semester Exams | - | Assessment 3: Mid semester exam (20%) |
9 | TOPIC 7: DNA replication |
Prac Test Rapid Biuret Protein Determination |
Practical test (C/NYC) |
10 | TOPIC 8: Transcription | Extraction of pea DNA | Logbook |
11 | TOPIC 9: Translation | DNA extraction/ Electrophoresis of Dyes | Logbook |
Student vacation | Student vacation | - | |
12 | TOPIC 10: Catabolic pathways | DNA extraction/ Electrophoresis of Dyes |
Logbook |
13 | TOPIC 11: Biosynthetic pathways | DNA: Nuclease digest and electrophoresis/ DNA Spectrophotometric Determination |
Logbook |
14 | TOPIC 12: Clinical Biochemistry | DNA: Nuclease digest and electrophoresis/ DNA Spectrophotometric Determination |
Logbook |
15 | Revision | DNA tidy up and Sup Exam Biuret | Hand in logbook (C/NYC) |
16 | End of Semester Exam | - | Assessment 4: End of Semester exam (20%) |
Learning Resources
Prescribed Texts
References
Other Resources
Overview of Assessment
Assessments for this course typically consist of:
• Class activities
• Assignments
• Presentations
• Exams
Assessment Tasks
- Assessment 1: Practical Report (Precipitation of protein) 7.5%
- Assessment 2: Practical Report (Biuret Weeks 2-7) 12.5%
- Assessment 3: Mid semester exam (20%)
- Assessment 4: End of Semester exam (20%)
Assessment Matrix
Other Information
Assessment Information
• To pass the course you need to pass, on average, each type of assessment (exam, prac reports, and assignments etc.). For example, if there are two tests you need to have an average of 50% to pass. But you also need to have passed the other assessments as well. You can’t make up marks from one type of assessment to another (eg pass the tests but fail the prac reports).
• Late work that is submitted without an application for an extension will not be corrected.
• Extension of time for submission of assessable work- A student may apply for an extension of up to 7 days from the original due date. They must lodge the application form (available on the web http://mams.rmit.edu.au/seca86tti4g4z.pdf) at least the day before the due date. The application is lodged with the School Admin Office on Level 6, Bdg 51. Students requiring longer extensions must apply for Special Consideration (form available on the Web). For missed assessments such as exams- you (& your doctor if you are sick) must fill out a special consideration form. This form must be lodged at the HUB or online with supporting evidence (eg medical certificate), prior to, or within, 48 hours of the scheduled time of examination.
• In order to be successful in this module, you have to achieve a pass in all of the above assessment tasks. If you miss an assessment task due to unavoidable circumstances, you need to follow the procedure of special consideration and apply within the allowed time frame.
If you have any questions about this assessment process, feel free to email me on amberlee.mitton@rmit.edu.au
Course Overview: Access Course Overview