Course Title: Perform aseptic techniques
Part B: Course Detail
Teaching Period: Term1 2021
Course Code: BIOL5242C
Course Title: Perform aseptic techniques
School: 174T School of VE Engineering, Health & Science
Campus: City Campus
Program: C4397 - Certificate IV in Laboratory Techniques
Course Contact: Rauha Quazi
Course Contact Phone: +61 3 9925 4277
Course Contact Email: rauha.quazi@rmit.edu.au
Name and Contact Details of All Other Relevant Staff
Andrea McAdam
andrea.mcadam@rmit.edu.au
Nominal Hours: 40
Regardless of the mode of delivery, represent a guide to the relative teaching time and student effort required to successfully achieve a particular competency/module. This may include not only scheduled classes or workplace visits but also the amount of effort required to undertake, evaluate and complete all assessment requirements, including any non-classroom activities.
Pre-requisites and Co-requisites
None
Course Description
This unit of competency describes the skills and knowledge to perform aseptic techniques to maintain the integrity of both the sample source and the sample. It applies to sampling techniques in tissue culture and to generic microbiological procedures.
This unit of competency applies to laboratory assistants and technicians working in the field or laboratory in the biomedical, biological, food processing and environmental industry sectors.
National Codes, Titles, Elements and Performance Criteria
National Element Code & Title: |
MSL973016 Perform aseptic techniques |
Element: |
1. Prepare for aseptic sampling or transfer |
Performance Criteria: |
1.1 Check the sampling procedure conforms with the required sampling plan 1.2 Use specified personal protective equipment (PPE) 1.3 Prepare the work area for safe and effective sample transfer 1.4 Select equipment and materials specified by the procedure 1.5 Organise equipment to minimise contamination during manipulations 1.6 Label containers for clear identification 1.7 Record details in relevant log or database 1.8 Macroscopically assess media and/or reagents to confirm sterility before use |
Element: |
2. Transfer materials aseptically |
Performance Criteria: |
2.1 Protect the integrity of the sample source 2.2 Sterilise inoculating loops and/or pipette where used to prevent contamination 2.3 Perform transfer while minimising opportunities for contamination and cross-infection 2.4 Protect the integrity of the sample source and destination after transfer, and before sealing the transport or culture vessel 2.5 Re-sterilise inoculating loops, minimising the generation of aerosols as required 2.6 Perform quality control checks to confirm aseptic transfer was successful 2.7 Label transport or culture vessels for clear identification |
Element: |
3. Maintain work area and equipment to prevent cross-infection and contamination |
Performance Criteria: |
3.1 Place disposable and reusable items into relevant receptacles 3.2 Clean and disinfect work area and equipment after use 3.3 Transport disposable and reusable contaminated materials to relevant areas for disinfection, sterilisation and cleaning or disposal 3.4 Follow personal hygiene procedures |
Learning Outcomes
Details of Learning Activities
Learning activities include interactive online classes, practical laboratory exercises, and individual study
Teaching Schedule
Week |
2021 |
Laboratory Practical Classes |
Theory Topics |
Assessments |
|
1 |
MONDAY |
ONLINE DRY LAB |
A1-1 Introduction to aseptic technique A1-2 Patient specimens |
AT2 Log book |
|
2 |
15 Feb |
PRAC 1 -1 |
PRAC 1 Introduction to aseptic techniques and lab 1. Using loop
|
A2-1 Culture techniques A2-2 Sterilisation & autoclaves
|
WORKSHEET A1 due 21/2 Log book |
3 |
22 Feb |
PRAC 1 -2 |
A2-3 Bacteria Prepare: Streaking for single colonies
|
WORKSHEET A2 due 28/2 Log book |
|
4 |
1 Mar |
PRAC 2 -1 |
PRAC 2 Streaking & swabbing 1. Review results of PRAC 1 2. Streak for single colonies 3. Environmental sample |
A3-1 Media |
Log book |
5 |
8 Mar |
NO CLASSES MONDAY |
A3-2 Disinfection A3-3 Cross-contamination |
WORKSHEET A3 due 14/3 Log book |
|
6 |
15 Mar |
PRAC 2 -2 |
A4-1 Reading plates A4-2 Gram staining |
|
|
7 |
22 Mar |
PRAC 3 -1 |
PRAC 3 More streaking plus colony morphology 1. Review results of PRAC 2 2. Streak for pure culture 3. Describe colonies on media |
A5-1 Antibiotic sensitivities A5-2 Biosafety cabinets |
WORKSHEET A4 done in lab PRAC 3 Practice Quiz WORKSHEET A5 due 8/3 |
8a |
29 Mar |
PRAC 3 -2 |
TUES 30 Mar Revision exercises to prepare for Quiz assessment FRI 9 April |
|
|
8b |
[5 April] |
Mid semester break |
ALL RE-SUBMISSIONS & CORRECTIONS |
||
9 |
12 April |
PRACTICAL ASSESSMENT all groups |
KNOWLEDGE ASSESSMENT Online quiz in class time |
AT1 Practical Assessment AT3 Knowledge Test |
Learning Resources
Prescribed Texts
References
Other Resources
Overview of Assessment
Assessments will include observation of skills in the laboratory, logbook and theory test
Assessment Tasks
Assessment Task 1: Practical
AT1 Lab skills assessment
Observed performance of laboratory tasks
Date: Final week
Assessment Task 2: Portfolio
AT2 Logbook and worksheets
Record of laboratory results (practical lab and online work)
Due: Submitted weekly; Final week is last date for corrections
Assessment Task 3: Knowledge
AT3 Theory test
Summative online quiz – three attempts
Date: Final week
Assessment Matrix
Other Information
This course is graded in accordance with competency-based assessment
CA Competency Achieved
NYC Not Yet Competent
DNS Did Not Submit for assessment
To pass the course you need achieve a satisfactory result for all assessments. Students may be given additional opportunities to demonstrate competence.
Late work that is submitted without an application for an extension (see below) will not be corrected.
APPLICATION FOR EXTENSION OF TIME FOR SUBMISSION OF ASSESSABLE WORK - A student may apply for an extension of up to 7 days from the original due date. They must lodge the application form (https://www.rmit.edu.au/students/student-essentials/assessment-and-results/extensions-of-time-for-submission-of-assessable-work) at least 24 hours before the due date. The application should be emailed to the program Coordinator (rauha.quazi@rmit.edu.au). Students requiring longer extensions must apply for SPECIAL CONSIDERATION (see below).
• For missed assessments such as tests- you (& your doctor if you are sick) must fill out a special consideration form. This form must be lodged online with supporting evidence prior to, or within, 5 days of the scheduled time of the assessment (https://www.rmit.edu.au/students/student-essentials/assessment-and-results/special-consideration)
• Plagiarism is the presentation of the work, idea or creation of another person as though it is your own. It is a form of cheating and is a very serious academic offence that may lead to expulsion from the University. Plagiarised material can be drawn from, and presented in, written, graphic and visual form, including electronic data and oral presentation. Plagiarism occurs when the origin of the material used is not appropriately cited. It also occurs through enabling plagiarism, which is the act of assisting or allowing another person to plagiarise or to copy your own work. Please make sure you consider this carefully in completing all your work and assessments in this course and if you are unsure about whether you might have plagiarised, seek help from your teacher.
Course Overview: Access Course Overview