Course Title: Prepare tissue and cell cultures
Part B: Course Detail
Teaching Period: Term1 2010
Course Code: ONPS5208C
Course Title: Prepare tissue and cell cultures
School: 155T Vocational Health and Sciences
Campus: City Campus
Program: C5183 - Diploma of Laboratory Technology (Biotechnology)
Course Contact: Geraldene Fleishman
Course Contact Phone: +61 3 9925 4287
Course Contact Email: geraldene.fleishman@rmit.edu.au
Name and Contact Details of All Other Relevant Staff
Nominal Hours: 50
Regardless of the mode of delivery, represent a guide to the relative teaching time and student effort required to successfully achieve a particular competency/module. This may include not only scheduled classes or workplace visits but also the amount of effort required to undertake, evaluate and complete all assessment requirements, including any non-classroom activities.
Pre-requisites and Co-requisites
PMLTEST305B - Perform aseptic techniques.
Course Description
This unit of competency covers the ability to prepare primary tissue cultures for applications, such as maintenance of animal cell lines and propagation of plants by tissue culture and basic subculture procedures. Personnel are required to manipulate equipment and materials and samples to prevent contamination at all preparation stages. They will have ready access to enterprise procedures and will work under direct supervision.
National Codes, Titles, Elements and Performance Criteria
National Element Code & Title: |
PMLTEST412A Prepare tissue and cell cultures |
Element: |
Maintain records |
Performance Criteria: |
5.1 Maintain records of batches of media and test data |
Element: |
Monitor tissue or cell culture |
Performance Criteria: |
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Element: |
Prepare and test cell and tissue culture media |
Performance Criteria: |
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Element: |
Prepare tissue or cell cultures |
Performance Criteria: |
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Element: |
Work safely according to the legal and regulatory framework |
Performance Criteria: |
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Learning Outcomes
Details of Learning Activities
Learning activities for this course will include:
Teacher directed delivery of lessons
Whole class Discussion
Pair/group discussion and self assessment
Worksheets
Laboratory experiments
Record keeping of experiments
Research activities
See the Teaching Schedule (below) for details.
Teaching Schedule
Date | LECTURE TOPIC | PRACTICAL S and other tasks |
9.2 | Introduction to tissue culture and biotechnology | Lab equipment |
16.2 | Requirements of a tissue culture lab | Pipette prac and online pod cast |
23.2 | Culture environment and media I | Media preparation 1 |
2.3 | culture environment and media 2 | Spot test Media preparation 2 Pipette practical due |
9.3 | Media preparation and filtration | cell subculture practical group 1 |
16.3 | sterilisation and disinfection | cell subculture practical 2 hand back of pipette practical |
23.3 | cell growth, cell counts and dell lines | |
30.3 | revision on line | cell subculture practical due |
6.4 | Vacation | |
13.4 | Exams | |
20.4 | Characterization of cells | Release and talk about essay topic |
27.4 | Contamination |
Journal article round table |
4.5 | Cryopreservation |
demonstration of cell freezing |
11.5 | large scale tissue culture procedures |
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18.5 | Specialised cell lines |
ESSAY DUE |
25.5 | practical exam |
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1.6 | Revision |
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8.6 | Exam |
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16.6 | Additional assessment |
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Learning Resources
Prescribed Texts
References
Other Resources
Although you do not have to purchase the following book, it will be referred to quite often. All relevant pages will be made available to you:
Culture of Animal cells: A manual of basic technique, R Ian Freshney. Fifth edition Wiley -Liss Publications 2005
Overview of Assessment
Assessments for this course typically consist of:
• Class activities
• Assignments
• Presentations
• Exam
Assessment Tasks
Term one test worth 27.5 % of the total course
Term two test worth 27.5% of the total course
Practical test at the end of term two is worth 5% of the course
Essay due the 11.05.10 is worth 12.5% of the course
Talk on 11.05.10 is worth 12.5% of the course
Practical report one due on 02.03.10 is worth 5% of the whole course
Practical report two due on 13.04.10 is worth 10% of the course
Please not that this information is subject to change
Assessment Matrix
Other Information
Important Information
Submission of work & late work- Students are required to submit work on the due date at the School Office using the cover sheets provided. The work will be accepted through the School Office and you will receive a receipt which you should keep as proof of submission. Students must NOT submit work directly to the teacher.
Late submission of work will incur a penalty of 5% of the maximum mark per day unless Special Consideration is applied for in writing using the required form. Marks can be deducted until the minimum pass mark is reached, after which no further marks will be deducted. Work will not be accepted more than 1 week after the due date.
Special Consideration- Any student who, because of illness or other circumstances beyond their control, has been prevented from attending a test, or whose performance in a test was affected by illness or other circumstances beyond their control may apply for Special Consideration. This must be done within 48 hours of the missed assessment/ assignment by lodging the appropriate form (available at the 6th floor office) with medical certificate or other supporting documentation at the Reception Office on Level 6, Building 51. Note: It is the policy of the School of Life and Physical Sciences NOT to AUTOMATICALLY PROVIDE SUPPLEMENTARY OR DEFERRED EXAMS. You will be advised of the outcome of your application for Special Consideration. The taking of Annual Leave does NOT constitute grounds for special consideration.
Attendance Requirements- Student must attend 80% of practical classes to pass. Practical exercises will be chosen for presentation as formal reports. If they are not satisfactory, you will be given an opportunity to resubmit. If you are absent from a session nominated for a formal report and you have applied for special consideration, it is your responsibility to negotiate a replacement report with the teacher. You MUST submit the number of required reports in order to pass the subject.
Plagiarism- Plagiarism is the presentation of the work, idea or creation of another person as though it is your own. It is a form of cheating and is a very serious academic offence that may lead to expulsion from the University. Plagiarised material can be drawn from, and presented in, written, graphic and visual form, including electronic data and oral presentation. Plagiarism occurs when the origin of the material used is not appropriately cited.
It also occurs through enabling plagiarism, which is the act of assisting or allowing another person to plagiarise or to copy your own work. Please make sure you consider this carefully in completing all your work and assessments in this course and if you are unsure about whether you might have plagiarised, seek help from your teacher.
Students must follow all OH&S requirements for working in the PC2 laboratory
Course Overview: Access Course Overview