Course Title: Apply routine chromatographic techniques
Part B: Course Detail
Teaching Period: Term2 2011
Course Code: ONPS5211C
Course Title: Apply routine chromatographic techniques
School: 155T Vocational Health and Sciences
Campus: City Campus
Program: C5183 - Diploma of Laboratory Technology (Biotechnology)
Course Contact: Kirsten Balding
Course Contact Phone: +61 3 9925 4283
Course Contact Email: Kirsten.balding@rmit.edu.au
Name and Contact Details of All Other Relevant Staff
none
Nominal Hours: 120
Regardless of the mode of delivery, represent a guide to the relative teaching time and student effort required to successfully achieve a particular competency/module. This may include not only scheduled classes or workplace visits but also the amount of effort required to undertake, evaluate and complete all assessment requirements, including any non-classroom activities.
Pre-requisites and Co-requisites
PMLTEST303B - Prepare working solutions OR PMLTEST402B - Prepare, standardise and use solutions
PMLTEST404A - Perform chemical tests and procedures.
Course Description
This unit of competency covers the ability to analyse samples using routine chromatographic techniques. The unit also includes establishing client needs for routine and non-routine samples, optimising enterprise procedures and instruments for specific samples, obtaining valid and reliable data and reporting test results. Personnel are required to recognise atypical test data/results and troubleshoot common analytical procedure and equipment problems.
National Codes, Titles, Elements and Performance Criteria
National Element Code & Title: |
PMLTEST513A Apply routine chromatographic techniques |
Element: |
1. Establish client needs and schedule analysis |
Performance Criteria: |
1. Establish client needs and schedule analysis |
Learning Outcomes
Details of Learning Activities
Learning activities for this course will include:
• Teacher directed face-to face delivery of lessons
• Whole class Discussion
• Pair/group discussion
• Small group workshops
• Online quizzes & theory activities
• Worksheets including Revision
• Laboratory experiments
• Record keeping of experiments
• Presentations
• Research activities
See the Teaching Schedule (below) for details.
Teaching Schedule
Date (Mon) | Week | Theory (online) | Prac (4 hr) | Assessment |
11/7 |
1 | * Course Overview | Safety in the Chromatography Lab Making solutions |
Safety checks |
|
* Paper Chromatography Theory - concepts & calcs | |||
18/7 |
2 | Paper Chromatography Theory | Paper Chromatography of food dyes | |
25/7 |
3 | TLC Theory concepts & calcs | Thin Layer Chromatography of Vegetable pigments | Group 1 oral prac report* Week 3 Quiz |
1/8 |
4 | Gel Chromatography (Size Exclusion Chromatography (SEC)) Theory | SEC Chromatography | Group 2 oral prac report* |
8/8 |
5 |
Ion Exchange (IE) Chromatography Theory | Ion Exchange Chromatography | Group 3 oral prac report* Week 5 Quiz |
15/8 |
6 |
Affinity chromatography & Multistep chromatography - concepts Case study: CSL |
Multistep chromatography - Rainbow chromatography: isolating a mixture of food dyes | Group 4 oral prac report* Week 6 Quiz - CSL case study |
22/8 |
7 | Revision | no prac class |
|
Week of 29/8 |
8 | Exam Week |
Test 1 (theory) |
Test 1 |
5/9 |
9 | HPLC theory & calculations - peak area, retention time, internal standards Solid Phase Extraction (SPE) |
Solution Making for HPLC GFP &/or BFP from E. coli cells. |
Week 9 Quiz |
12/9 |
10 | HPLC - isocratic v’s gradient chromatography theory | 1. HPLC of caffeine in drinks |
Group 5 oral prac report* Prac Report 2 - caffeine in drinks |
Week of 19/9 |
Student vacation | |||
26/9 |
11 |
HPLC - isocratic v’s gradient chromatography theory |
1. HPLC of caffeine in drinks - interpreting results |
Prac Report 2 - caffeine in drinks |
3/10 |
12 | Example calcs for analgesics HPLC prac, interpreting results, practising calcs & answering Q’s |
HPLC of Analgesics (Painkiller) Tablets TLC of aspirin, caffeine & paracetamol |
Week 12 Quiz |
10/10 |
13 | Example calcs for analgesics HPLC prac, interpreting results, practising calcs & answering Q’s | HPLC of Analgesics (Painkiller) Tablets interpreting results | Submit Logbook |
1710 |
14 |
GC theory & calculations Internal standards |
GC @ Applied Chemistry Gas Chromatography of Xylene (to be confirmed) |
Week 14 Quiz |
24/10 |
15 | Revision | ||
Week of 31/10 |
16 | Exam Week | Test 2 |
Test 2 |
Week of 8/11 |
17 | Alternative assessment (Supplementary exams) if required |
||
|
* Oral practical report = powerpoint presentation to class |
Learning Resources
Prescribed Texts
Perform Routine Chromatographic Techniques Practical Manual (available online) |
|
• J Crowe, T. Bradshaw, P. Monk (2006) |
References
Other Resources
Overview of Assessment
Assessments for this course typically consist of:
• Class activities
• Assignments
• Presentations
• Exam
Assessment Tasks
1. Safety Checks
Week done: 1
Due: in class
%: satisfactory (ungraded)
2. Prac Report 1 - Oral group report (powerpoint presentation to class)
Week done: One group per week only
Topic: for experiments done during weeks 2, 3, 4, 5, and 9 to be given on previous week’s experiment
Week due: Group 1 - Week 3*/Group 2 - Week 4*/Group 3 - Week 5*/Group 4 - Week 6*/ Group 5 - Week 10*
%: 10%
3. Quizzes
Week done: 3,5,6,9,12,14
Topic: see lab manual ’assessment guide’ section
Week due: various - see blackboard
%: 20 (calculated from average grade)
4. Prac Report 2 (written group assignment)
Week done: 10/11
Topic: HPLC of caffeine in drinks
Week due: 13
%: 10%
5. Exam 1
Week done: 8
Topic: Chromatography (Paper, TLC, SEC, IEC, multistep)
n/a
%: 20%
6. Exam 2
Week done: 16
Topic: HPLC & GC only
Week due: n/a
%: 20%
7. Logbook
Week done: all
Requirement: Logbook - signed off if considered competent** by teacher & recorded on central record/ Sign off each week lab class is attended**
Week due: Submit completed logbook at end of week 13 (monday week 14 is last day to avoid penalty to grades)
%: 20
8. Input results to spreadsheet (LIMS)
Week done: During some prac classes - students will be advised when to do this, and more than one opportunity is available
Requirement: several accurate and timely entries of data into the LIMS
Week due: n/a
%: satisfactory
9. Attendance
Week done: all
Requirement: 80% attendance or more is required
Week due: n/a
%: satisfactory
Total 100%
Assessment Matrix
Other Information
*The topic of the presentation is 2 weeks before the talk. For example, TLC of vegetable pigments is done in week 3 lab class, the talk on this topic will be held in week 5. The presentation should contain a brief explanation of how the separation works, results and a short discussion of trends in the data and what the results tell you about the mixture being separated. Talks should be no more than 10 minutes long. All group members must present part of the talk.
** see logbook information for what is required.
IMPORTANT POINTS TO NOTE ABOUT ASSESSMENT CONTINUED…
*If you miss the experiment that you are to present as an oral presentation, you may present a different experiment as long as you provide a medical certificate
*Practical report 2 is due 2 weeks after the day that the practical (including analysis session) is performed.
*Due dates for quizzes: Quizzes are to be done before the class in the same week. <br>
NO QUIZ = NO ENTRY TO CLASS. This is for safety reasons.
*To pass the quizzes component you must get an average pass for all quizzes (ie. 50% average). The score for quizzes is calculated from the average grade of all quizzes. If you miss a quiz and have submitted a medical certificate you may do it later than the due date as long as you contact the teacher asap after your absence.
*Students must attend at least 80 % of practical classes to pass the course.
*Students must pass all of the assessment tasks to pass the course.
***Reports (powerpoint for oral presentation or word document for written report) can be submitted online via blackboard or by memory stick or email to the teacher if blackboard is not available. The online plagiarism statement given in blackboard must be included with the emailed assignment to show that the student agrees to these conditions***
Logbook
Your logbook must be signed off after completion each week by both the student AND the teacher.
The teacher will also signoff your logbook on the class sheet as a central record.
The central record is the evidence record that is required to show that your logbook has been signed off by you, the student, and the teacher.
IT IS THE STUDENT’S RESPONSIBILITY TO GET THEIR WORK SIGNED OFF EVERY WEEK.
(If you miss the class you do not need to get the sheet signed off – the teacher will record this as an absence). YOU WILL BE CONSIDERED NYC* FOR THAT WEEK UNTIL THE CENTRAL RECORD IS SIGNED OFF or YOU ARE MARKED ABSENT.
*NYC = not yet competent
When is a prac performance considered “competent’ (ie when can a logbook be signed off)?Logbooks will only be signed off when the student has followed and completed a method adequately, and is therefore considered competent for that experiment.
THE LOGBOOK WILL ONLY BE SIGNED OFF BY THE TEACHER AFTER THE FOLLOWING IS DONE:1. Show date of analysis/results at top of page at start of experimental record in logbook,
2. Attach loose pages to logbook securely (use tape or staples),
3. Include raw and analysed results in logbook as appropriate to particular method (see lab manual)
4. Clean up lab work area and equipment adequately
5. Student signed off logbook entry once record of results are completed and clean up done
When is a prac performance considered “not yet competent’?(ie when will a logbook not be signed off?)
A student is considered to be ‘not yet competent’ for an experiment if:*The Student doesn’t attend class long enough to complete the experiment
*The Student doesn’t finish the procedure in a reasonable time
(nominally by the time class finishes – excluding when there are logistic issues that mean few/no one in the class finishes).
*The student doesn’t contribute to the group effort adequately
*Results are a debacle – several errors/extensive lack of following SOP (method).
*The experiment is not conducted in a safe manner
*The lab area and equipment are not adequately cleaned up
In the case of a not yet competent result, it is noted that there is no opportunity to repeat that particular experiment due to scheduling of classes. In this case, to achieve competency other experiments during the course should be done to a ‘competent’ level and the student should show a good amount of improvement as semester goes along.
As evidenced by…
*Observations in class by the teacher (record reason for NYC grade)*The attitude and work ethic of the student while doing experiments is considered appropriate by the teacher
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