Course Title: Perform aseptic techniques

Part B: Course Detail

Teaching Period: Term1 2013

Course Code: ONPS5655C

Course Title: Perform aseptic techniques

School: 155T Vocational Health and Sciences

Campus: City Campus

Program: C5282 - Diploma of Laboratory Technology (Biotechnology)

Course Contact: Amberlee Mitton

Course Contact Phone: +61 3 9925 8053

Course Contact Email:

Name and Contact Details of All Other Relevant Staff

Teacher: Gwen MacDonald

Tel: 99254283

Nominal Hours: 40

Regardless of the mode of delivery, represent a guide to the relative teaching time and student effort required to successfully achieve a particular competency/module. This may include not only scheduled classes or workplace visits but also the amount of effort required to undertake, evaluate and complete all assessment requirements, including any non-classroom activities.

Pre-requisites and Co-requisites

there are no pre-requisites or co-requisites for this course


Course Description

This unit of competency covers the ability to perform aseptic techniques to maintain the integrity of both the sample source and the sample. It applies to sampling techniques in tissue culture and to generic microbiological procedures.


National Codes, Titles, Elements and Performance Criteria

National Element Code & Title:

MSL973004A Perform aseptic techniques


1. Prepare for aseptic sampling or transfer

Performance Criteria:

1.1. Ensure that any sampling procedure conforms with the requirements of the sampling plan
1.2. Use specified personal protective clothing and equipment
1.3. Prepare the work area for safe and effective sample transfer
1.4. Select equipment and materials specified by the procedure
1.5. Organise equipment to minimise contamination during manipulations
1.6. Label containers for clear identification
1.7. Record details in relevant log or database


2. Transfer materials aseptically

Performance Criteria:

2.1. Protect the integrity of the sample source by sterilising the sampling site and the mouth of transport or culture vessel
2.2. Sterilise inoculating loops and/or pipette where used to prevent contamination
2.3. Perform transfer while minimising opportunities for contamination and cross-infection
2.4. After transfer, and before sealing the transport or culture vessel, flame the vessel mouth to maintain sterility
2.5. Re-sterilise inoculating loops, minimising the generation of aerosols
2.6. Perform quality control checks, if required
2.7. Label transport or culture vessels for clear identification


3. Maintain work area and equipment to prevent cross-infection and contamination

Performance Criteria:

3.1. Place disposable and reusable items into relevant receptacles
3.2. Clean and disinfect work area and equipment after use
3.3. Transport disposable and reusable contaminated materials to relevant areas for disinfection, sterilisation and cleaning or disposal

Learning Outcomes

Details of Learning Activities

Practical classes in laboratory

tutorials, short talks

On line quizzes 

Teaching Schedule

15.4.13Cross infection prac

Patient specimen requirements
autoclave theory
Plating specimens onto HBA
Gram stain


29.4.13Read plates
Media making
Autoclave demonstration and use

6.5.13Sterilisation/disinfection Disinfection theory
Plating out

13.5.13Gram stain/
Plating out
20.5.13Biohazard cab theory
Read plates
Patient swabs

27.5.13Tutorial technique/microscopy
Read plates
31.5.13Practical competency assessment

Final Practical assessment

Learning Resources

Prescribed Texts


Other Resources

Access to internet and library

Overview of Assessment

Assessments for this course typically consist of:
• Class activities
• Assignments
• Presentations
• Exams

Assessment Tasks

Short quizzes on line  ( Pass grade needed)
Work book - record of practicals done signed  and of satisfactory standard

Practical report- satisfactory  due 6.5.13  

 Practical assessment of aseptic procedures as described in learning elements will occur in practical classes and during formal practical assessment

 Competent or not yet competent grading . no higher grading available

To pass the course you need to pass, on average, each type of assessment (tests, reports, assignments)
For example, if there are two tests you need to have an average of 50% to pass. But you also need to have passed the other assessments as well. Students must attend at least 80% of practical classes. If you miss a prac class you should provide a copy of your medical certificate or other evidence (court appearance, funeral notice, statutory declaration) to the teacher.

Extension of time for submission of assessable work-

A student may apply for an extension of up to 7 days from the original due date . They must lodge the application form (available on the web) at least the day before the due date. The application is lodged with the School Admin Office on Level 6, Bdg 51

Students requiring longer extensions must apply for Special Consideration (form available on the Web)

For missed assessments such as exams- you (& your doctor if you are sick) must fill out a special consideration form. This form must be lodged online with supporting evidence (eg medical certificate),  within, 48 hours of the scheduled time of examination.

Late work submitted without forms will not be marked.

Assessment Matrix

Other Information

This course is taught concurrently with microscopy techniques ONPS5656C

80% of practical classes need to be attended to pass this course 

Course Overview: Access Course Overview