Course Title: Perform aseptic techniques

Part B: Course Detail

Teaching Period: Term1 2015

Course Code: ONPS5655C

Course Title: Perform aseptic techniques

School: 155T Vocational Health and Sciences

Campus: City Campus

Program: C5282 - Diploma of Laboratory Technology (Biotechnology)

Course Contact: Amberlee Mitton

Course Contact Phone: +61 3 9925 8053

Course Contact Email:

Name and Contact Details of All Other Relevant Staff

Andrea McAdam

Nominal Hours: 40

Regardless of the mode of delivery, represent a guide to the relative teaching time and student effort required to successfully achieve a particular competency/module. This may include not only scheduled classes or workplace visits but also the amount of effort required to undertake, evaluate and complete all assessment requirements, including any non-classroom activities.

Pre-requisites and Co-requisites

there are no pre-requisites or co-requisites for this course


Course Description

This unit of competency covers the ability to perform aseptic techniques to maintain the integrity of both the sample source and the sample. It applies to sampling techniques in tissue culture and to generic microbiological procedures.


National Codes, Titles, Elements and Performance Criteria

National Element Code & Title:

MSL973004A Perform aseptic techniques


1. Prepare for aseptic sampling or transfer

Performance Criteria:

1.2. Use specified personal protective clothing and equipment
1.3. Prepare the work area for safe and effective sample transfer
1.4. Select equipment and materials specified by the procedure
1.5. Organise equipment to minimise contamination during manipulations
1.6. Label containers for clear identification
1.7. Record details in relevant log or database


2. Transfer materials aseptically

Performance Criteria:

2.1. Protect the integrity of the sample source by sterilising the sampling site and the mouth of transport or culture vessel
2.2. Sterilise inoculating loops and/or pipette where used to prevent contamination
2.3. Perform transfer while minimising opportunities for contamination and cross-infection
2.4. After transfer, and before sealing the transport or culture vessel, flame the vessel mouth to maintain sterility
2.5. Re-sterilise inoculating loops, minimising the generation of aerosols
2.6. Perform quality control checks, if required
2.7. Label transport or culture vessels for clear identification


3. Maintain work area and equipment to prevent cross-infection and contamination

Performance Criteria:

3.1. Place disposable and reusable items into relevant receptacles
3.2. Clean and disinfect work area and equipment after use
3.3. Transport disposable and reusable contaminated materials to relevant areas for disinfection, sterilisation and cleaning or disposal

Learning Outcomes

Details of Learning Activities

Practical classes in simulated laboratory

On line information

Teaching Schedule

Week beginningPracticalNotes

Autoclave use
Media making

27.4.15Disinfection effectiveinessSterilisation and Disinfection

Plating swabs
Gram Stain

Stain Theory

Biohazard Cabinet

Sensitivity testing
Biohazard Cabinet use


Reading plates
Plating out

25.5.15Practical evaluation
Final workbook evaluation

Cross infection

1.6.15On line quiz 

Learning Resources

Prescribed Texts


Other Resources

Access to internet and library

Overview of Assessment

Assessments for this course typically consist of:
• Class activities
• Assignments
• Presentations
• Exams

Assessment Tasks

Practical assessment of aseptic (in class and at end of semester) ( S/NYS)

Work book with accurate and adequate recording of results (S/NYS)

Glossary completed (S/NYS)

On line Quiz  (S/NYS)

Assessment Matrix

Other Information

Assessment Information

  • All assessment types must be passed (exams, prac, and assignments etc.). For example, if there are two tests you need to have an average of 50% to pass. You can’t make up marks from one type of assessment to another (e.g. pass the tests but fail the prac component).
  • Attendance at practical classes is compulsory. A minimum of 80% attendance is required to pass this course. If you are sick you should submit a medical certificate to 6th floor office addressed to the Program Coordinator
  • Late work that is submitted without an application for an extension will not be corrected.
  • APPLICATION FOR EXTENSION OF TIME FOR SUBMISSION OF ASSESSABLE WORK- A student may apply for an extension of up to 7 days from the original due date. They must lodge the application form (available online at least 24 hours before the due date. The application is lodged with the School Admin Office on Level 6, Bdg 51, or emailed to the Coordinator (
  • Students requiring extensions longer than 7 days must apply for Special Consideration (see the ‘Help me’ link in blackboard, via myRMIT studies or For missed assessments such as exams - you (& your doctor if you are sick) must fill out a special consideration form. This form must be lodged online with supporting evidence (e.g. medical certificate), prior to, or within, 48 hours of the scheduled time of examination.
  • If you miss an assessment task due to unavoidable circumstances, you need to follow the procedure of special consideration and apply within the allowed time frame.

This is Pass only subject- no higher grading

Course Overview: Access Course Overview