Course Title: Prepare tissue and cell cultures

Part B: Course Detail

Teaching Period: Term1 2012

Course Code: ONPS5661C

Course Title: Prepare tissue and cell cultures

School: 155T Vocational Health and Sciences

Campus: City Campus

Program: C5282 - Diploma of Laboratory Technology (Biotechnology)

Course Contact: Amberlee Mitton

Course Contact Phone: +61 3 9925 8053

Course Contact Email: amber.mitton@rmit.edu.au


Name and Contact Details of All Other Relevant Staff

Teacher: Suzie Politis

Nominal Hours: 50

Regardless of the mode of delivery, represent a guide to the relative teaching time and student effort required to successfully achieve a particular competency/module. This may include not only scheduled classes or workplace visits but also the amount of effort required to undertake, evaluate and complete all assessment requirements, including any non-classroom activities.

Pre-requisites and Co-requisites

ONPS5655C Perform Aseptic techniques

ONPS 5656C Microscopy

Course Description

This unit of competency covers the ability to prepare primary tissue cultures for applications, such as maintenance of animal cell lines and propagation of plants by tissue culture and basic subculture procedures. Personnel are required to manipulate equipment and materials and samples to prevent contamination at all preparation stages. They will have ready access to enterprise procedures and will work under direct supervision.


National Codes, Titles, Elements and Performance Criteria

National Element Code & Title:

MSL974011A Prepare tissue and cell cultures

Element:

1. Work safely according to the legal and regulatory framework

Performance Criteria:

1.1 Ensure work practices and personal actions conform to regulations, codes, guidelines and enterprise quality assurance procedures
1.2 Identify hazards and enterprise controls associated with the sample, preparation methods, reagents and equipment
1.3 Select, fit and use personal protective clothing and safety equipment
1.4 Address hazards and incidents as they arise
1.5 Maintain a chain of custody, traceable to the worker, for all cells and tissues

Element:

2.Prepare and test cell and tissue culture media

Performance Criteria:

2.1 Select and confirm media specifications and processes/methods
2.2 Prepare culture media to suit the application
2.3 Sterilise culture media and check for sterility
2.4 Perform quality control checks to ensure that culture media is fit for purpose
2.5 Store culture media in accordance with specifications

Element:

3. Prepare tissue or cell cultures

Performance Criteria:

3.1 Select tissue/cell sample to optimise growth and prepare it for culture
3.2 Add specified growth agents and/or nutrients
3.3 Inoculate culture medium using aseptic techniques

Element:

4. Monitor tissue or cell culture

Performance Criteria:

4.1 Incubate culture in specified conditions
4.2 Monitor growth of culture and record appearance and characteristics
4.3 Report presence or absence of contamination
4.4 Subculture the culture to continue the cell line
4.5 Dispose of biohazardous and other laboratory waste safely

Element:

5. Maintain records

Performance Criteria:

5.1 Maintain records of batches of media and test data
5.2 Ensure records of tissue cultures are retrievable,legible and accurate
5.3 Ensure records conform to information management,records, quality system and legal requirements

Learning Outcomes



Details of Learning Activities

• Teacher directed delivery of lessons• Whole class Discussion
• Pair/group discussion and self assessment
• Worksheets
• Laboratory experiments
• Record keeping of experiments
• Research activities



Teaching Schedule

Date Theory

1 7.2.2012
Introduction to tissue culture and aseptic technique
http://www.youtube.com/watch?v=yJ_acpKglto&feature=related

2 14.2.2012 The Tissue Culture Laboratory


3 21.2.2012
Plant Tissue culture environment

4 28.2.2012
Media for plant tissue culture
www.youtube.com/watch?v=yJ_acpKglto&feature=related

5 6.3.2012
Growth regulators
• Auxins
• Cytokinins
• Gibberellins
• Abscisic acid
• Ethylene

6 13.3.2012
Plant TC techniques I

7 20.3.2012
Plant TC techniques II

8 27.3.2012 REVISION

9 3.4.2012 Mid semester exam

10 10.4.2012 STUDENT VACATION

12 17.4.2012 Transgenic crops

13 24.4.2012 Introduction to animal tissue culture

14 1.5.2012 Culture environment and Media I

15 8.5.2012 Culture environment and Media II

16 15.5.2012 Media preparation and filtration

17 22.5.2012 REVISION

18 29.5.2012 Practical exam
19 5.6.2012 End of semester exam
12.6.2012 Additional assessment

Note that schedule may be subject to change


Learning Resources

Prescribed Texts


References


Other Resources


Overview of Assessment

Asessment for this course may include tests, practical reports, assignments and presentations


Assessment Tasks

Exam ONE 20%
Exam TWO 20%
Practical exam 20%
Essay 10%
Practical Report ONE 10%
Practical Report TWO 10%
Logbook 10%
Assessment tasks will assess the student’s ability to demonstrate the following competencies:

  • OH&S requirements and code of conduct in a tissue culture laboratory
  • ability to prepare reagents and tissue culture media accordingly
  • culturing cells aseptically
  • monitoring the growth of cultures and distinguishing any normal from abnormal cells as well as contamination
  • proper record maintenance


Assessment Matrix

Course Overview: Access Course Overview