Course Title: Prepare tissue and cell cultures

Part B: Course Detail

Teaching Period: Term1 2015

Course Code: ONPS5661C

Course Title: Prepare tissue and cell cultures

School: 155T Vocational Health and Sciences

Campus: City Campus

Program: C5282 - Diploma of Laboratory Technology (Biotechnology)

Course Contact: Amberlee Mitton

Course Contact Phone: +61 3 9925 8053

Course Contact Email: amber.mitton@rmit.edu.au


Name and Contact Details of All Other Relevant Staff

Teacher: Muriel Aldunate

muriel.aldunate@rmit.edu.au

Nominal Hours: 50

Regardless of the mode of delivery, represent a guide to the relative teaching time and student effort required to successfully achieve a particular competency/module. This may include not only scheduled classes or workplace visits but also the amount of effort required to undertake, evaluate and complete all assessment requirements, including any non-classroom activities.

Pre-requisites and Co-requisites

ONPS5655C Perform Aseptic techniques

ONPS 5656C Microscopy

Course Description

This unit of competency covers the ability to prepare primary tissue cultures for applications, such as maintenance of animal cell lines and propagation of plants by tissue culture and basic subculture procedures. Personnel are required to manipulate equipment and materials and samples to prevent contamination at all preparation stages. They will have ready access to enterprise procedures and will work under direct supervision.


National Codes, Titles, Elements and Performance Criteria

National Element Code & Title:

MSL974011A Prepare tissue and cell cultures

Element:

1. Work safely according to the legal and regulatory framework

Performance Criteria:

1.1. Ensure work practices and personal actions conform
to regulations, codes, guidelines and enterprise
quality assurance procedures
1.2. Identify hazards and enterprise controls associated
with the sample, preparation methods, reagents and
equipment
1.3. Select, fit and use personal protective clothing and
safety equipment
1.4. Address hazards and incidents as they arise
1.5. Maintain a chain of custody, traceable to the worker,
for all cells and tissues

Element:

2.Prepare and test cell and tissue culture media

Performance Criteria:

2.1. Select and confirm media specifications and
processes/methods
2.2. Prepare culture media to suit the application
2.3. Sterilise culture media and check for sterility
2.4. Perform quality control checks to ensure that culture
media is fit for purpose
2.5. Store culture media in accordance with
specifications

Element:

3. Prepare tissue or cell cultures

Performance Criteria:

3.1. Select tissue/cell sample to optimise growth and
prepare it for culture
3.2. Add specified growth agents and/or nutrients
3.3. Inoculate culture medium using aseptic techniques

Element:

4. Monitor tissue or cell culture

Performance Criteria:

4.1. Incubate culture in specified conditions
4.2. Monitor growth of culture and record appearance
and characteristics
4.3. Report presence or absence of contamination
4.4. Subculture the culture to continue the cell line
4.5. Dispose of biohazardous and other laboratory waste
safely

Element:

5. Maintain records

Performance Criteria:

5.1. Maintain records of batches of media and test data
5.2. Ensure records of tissue cultures are retrievable,
legible and accurate
5.3. Ensure records conform to information management,
records, quality system and legal requirements

Learning Outcomes



Details of Learning Activities

  • Teacher directed delivery of lessons
  • Whole class discussion
  • Pair/group discussion and self assessment
  • Worksheets
  • Laboratory experiments
  • Record keeping of experiments
  • Research activities


 


Teaching Schedule

Week Topic/Overview Practical/Activity/PresentationAssessment12345678 910111213141516
Topic 1: Intro to Biohazard cabinets, aseptic technique and tissue culture

PRAC 1: Introduction to the Biohazard hood, aseptic technique and tissue culture
 

  Log book
Topic 2: Introduction to plant tissue culture & plant physiology

PRAC 2: Seed (Lepidium sativum) propagation

PRAC 3: Media preparation
 

  Log book
Topic 3: Plant growth

 PRAC 4: Lepidium sativum seedling transfer

PRAC 5: Surface sterilisation preparation

  Log book
 
Topic 4: Plant reproduction

PRAC 6: Surface sterilisation and explant of African Violet leaves

  Log book
Topic 5: Tissue culture environment PRAC 7: Effect of surface sterilisation and explant of Petunia leaves
& Collect results from prac 6		  Log book
Topic 6: Media & Growth Regulators

PRAC 8: Collect results from PRAC 7 and compare results from PRAC 6&7

Discussion of PRAC results and Assessment 1

Assessment 1: Prac report 1 (PRAC 6, 7, 8 & 9) DUE: Week 10 (30%)

Log book
 Exam Week

 No Prac
 
Topic 7: Micropropagation 1  PRAC 9: Cauliflower explant  Log book
Student Vacation    
Topic 8: Micropropagation 2 PRAC 10: Carrot callus culture   
 Log book
Topic 9: Contamination PRAC 11: Succulent explant  
Log book
 Topic 10 - GMO Assessment 2 preparation  
 - PRAC 12: Collect PRAC 10 & 11 results and take succulents home  
Log book
-

Group presentations
 

  Assessment 2: Group presentation:
DUE: Draft Week 11
Presentation: Week 13 (30%)
 
  Revision

Log book check and feedback 
 

 

 Assessment 3: Log Book – Competency based assessment (C/NYC) (Observational and not keeping skills)
DUE Date: Week 15
Log
 
 - Practical exam (Explant)


 Assessment 4: Practical Exam – Competency based assessment (C/NYC) (Technical skills)
End of Semester Exam No Practical Assessment 5: End of year quiz (40%)


Learning Resources

Prescribed Texts


References


Other Resources


Overview of Assessment

Asessment for this course may include tests, practical reports, assignments and presentations


Assessment Tasks

Assessment 1: Prac report (30%)

Assessment 2: Group presentation (30%)

Assessment 3: Practical Exam ( S/NYS)

Assessment 4: Log Book (S/NYS)

Assessment 5: End of year quiz (40%)


Assessment tasks will assess the student’s ability to demonstrate the following competencies:

  • OH&S requirements and code of conduct in a tissue culture laboratory
  • Ability to prepare reagents and tissue culture media accordingly
  • Culturing cells aseptically
  • Monitoring the growth of cultures and distinguishing any normal from abnormal cells as well as contamination
  • Proper record maintenance and analysis of results


Assessment Matrix

Other Information


Assessment Information
• This course is graded in accordance with competency-based assessment, but which also utilise graded assessment
CHD Competent with High Distinction (80 – 100%)
CDI Competent with Distinction (60 – 79%)
CC Competent with Credit (50 – 59%)
CAG Competency Achieved – Graded (0 – 49%)
NYC Not Yet Competent
DNS Did Not Submit for assessment

• To pass the course you need to pass, on average, each type of assessment (exams, pracs, and assignments etc.) For example, if there are two exams you need to have an average of 50% to pass and you also need to have passed the other assessment types. You can’t make up marks from one type of assessment to another (e.g. pass the exams but fail the prac component).
• Late work that is submitted without an application for an extension (see below) will not be corrected.
• APPLICATION FOR EXTENSION OF TIME FOR SUBMISSION OF ASSESSABLE WORK - A student may apply for an extension of up to 7 days from the original due date. They must lodge the application form (available online http://www1.rmit.edu.au/students/assessment/extension) at least 24 hours before the due date. The application is lodged with the School Admin Office on Level 6, Bdg 51. Students requiring longer extensions must apply for SPECIAL CONSIDERATION.
• For missed assessments such as exams- you (& your doctor if you are sick) must fill out a special consideration form. This form must be lodged online with supporting evidence prior to, or within, 48 hours of the scheduled time of examination http://www1.rmit.edu.au/students/specialconsideration
 

Course Overview: Access Course Overview