Course Title: Apply routine chromatographic techniques
Part B: Course Detail
Teaching Period: Term2 2016
Course Code: ONPS5663C
Course Title: Apply routine chromatographic techniques
School: 155T Vocational Health and Sciences
Campus: City Campus
Program: C5282 - Diploma of Laboratory Technology (Biotechnology)
Course Contact: Amberlee Mitton
Course Contact Phone: +61 3 9925 8053
Course Contact Email: amber.mitton@rmit.edu.au
Name and Contact Details of All Other Relevant Staff
Kirsten Balding
kirsten.balding@rmit.edu.au
contact by email, or talk in class
Nominal Hours: 120
Regardless of the mode of delivery, represent a guide to the relative teaching time and student effort required to successfully achieve a particular competency/module. This may include not only scheduled classes or workplace visits but also the amount of effort required to undertake, evaluate and complete all assessment requirements, including any non-classroom activities.
Pre-requisites and Co-requisites
MSL974003A Perform chemical tests and procedures
MSL973007A Perform microscopic examination
MSL974006A Perform biological procedures
MSL974001A Prepare, standardise and use solutions
Course Description
This unit of competency covers the ability to analyse samples using routine chromatographic techniques.
The unit also includes establishing client needs for routine and non-routine samples, optimising enterprise procedures and instruments for specific samples, obtaining valid and reliable data and reporting test results.
Personnel are required to recognise atypical test data/results and troubleshoot common analytical procedure and equipment problems.
National Codes, Titles, Elements and Performance Criteria
National Element Code & Title: |
MSL975009A Apply routine chromatographic techniques |
Element: |
1. Establish client needs and schedule analysis |
Performance Criteria: |
1.1. Liaise with client or sample provider to determine client needs and sample history 1.2. Record sample description, compare with specification and record and report discrepancies 1.3. Identify non-routine samples and the possible need to vary enterprise procedures 1.4. Seek advice from supervisor about any proposed variations and document all approved changes 1.5. Schedule analysis using enterprise procedures |
Element: |
2. Prepare samples and standards |
Performance Criteria: |
2.1. Obtain a representative analytical portion of the laboratory sample 2.2. Prepare sample in accordance with testing requirements 2.3. Prepare validation checks for analytical portion |
Element: |
3. Set up and optimise instrument |
Performance Criteria: |
3.1. Perform pre-use and safety checks in accordance with enterprise procedures 3.2. Start up and condition the instrument using enterprise procedures 3.3. Optimise instrumental parameters to suit sample and test requirements 3.4. Check calibration status of instrument and perform calibration using specified standards and procedures, if applicable |
Element: |
4. Perform analysis |
Performance Criteria: |
4.1. Measure analyte response for standards, validation checks and samples 4.2. Conduct sufficient measurements to obtain reliable data 4.3. Return instruments to standby or shutdown condition, as required |
Element: |
5. Process and analyse data |
Performance Criteria: |
5.1. Confirm data is the result of valid measurements 5.2. Perform required calculations and ensure results are consistent with standards or estimations and expectations 5.3. Record results with the appropriate accuracy, precision and units 5.4. Analyse trends in data and/or results and report out of specification or atypical results promptly to appropriate personnel 5.5. Troubleshoot analytical procedure or equipment problems which have led to atypical data or results |
Element: |
6. Maintain a safe work environment |
Performance Criteria: |
6.1. Identify risks, hazards, safety equipment and control measures associated with sample handling, preparation and analytical method 6.2. Use personal protective equipment and safety procedures specified for test method and materials to be tested 6.3. Minimise the generation of wastes and environmental impacts 6.4. Ensure the safe disposal of laboratory wastes 6.5. Clean, care for and store equipment and consumables in accordance with enterprise procedures |
Element: |
7. Maintain laboratory records |
Performance Criteria: |
7.1. Enter approved data and results into laboratory information management system (LIMS) 7.2. Maintain equipment logs in accordance with enterprise procedures 7.3. Maintain security and confidentiality of laboratory data and enterprise information 7.4. Communicate results to appropriate personnel |
Learning Outcomes
Details of Learning Activities
Learning activities for this course will include:
• Teacher directed face-to face delivery of lessons
• Whole class Discussion
• Pair/group discussion
• Small group workshops
• Online quizzes & theory activities
• Worksheets including Revision
• Laboratory experiments
• Record keeping of experiments
• Presentations
• Research activities
Teaching Schedule
TERM 1
Week |
Date week of |
Theory (online) |
Lab (Monday 4 hr) |
Assessment |
1 |
4-Jul
|
Topic 1 Paper Chromatography Theory - concepts & calcs |
Lab 1
|
Logbook |
2 |
11-Jul |
Topic 2 Paper Chromatography Theory |
Lab 2 Paper Chromatography of food dyes- (Method optimisation & effect of the solvent) |
Logbook Prac Report 1 |
3 |
18-Jul |
Topic 3 TLC Theory concepts & calcs |
Lab 3 Interpreting results |
Quiz 1 Logbook Prac Report 1 (part 2 analysis) |
4 |
25-Jul |
Topic 4 Size Exclusion Chromatography (SEC) Theory (also called gel filtration chromatography (GFC) |
Lab 4 Thin Layer Chromatography of Vegetable pigments (Method optimisation & effect of the solvent) |
Logbook |
5 |
1-Aug |
Topic 5 |
Lab 5 1. Solution Making for HPLC 2. SEC extraction of GFP and/or BFP from E. coli cells. Edvotek kit |
Quiz 2 Logbook |
6 |
8-Aug |
Topic 6
|
Lab 6
VALID METHOD |
|
7 |
15-Aug |
Revision |
Logbook assessment catchup |
|
8 |
22-Aug |
Exam Week |
|
Exam 1 |
Week of
|
Student vacation |
|
|
Subject to change
TERM 2
Week |
Date week of |
Theory (online) |
Prac (4 hr) |
Assessment |
||
9 |
5-Sep
|
Topic 7 HPLC theory & calculations - peak area, retention time, internal standards
|
Lab 7 Ion Exchange Chromatography
|
Quiz 3 Logbook |
||
10 |
12-Sep |
Topic 8 HPLC - isocratic v's gradient chromatography theory
|
Lab 8 HPLC of caffeine in drinks (whole class) Case studies – Common problems and Troubleshooting |
Prac Report 2 Logbook |
||
11 |
19-Sep |
Topic 9 Caffeine in Drinks - Using standards to find the concentration of a substance |
Lab 9 1. HPLC of caffeine in drinks - interpreting results |
Prac Report 2 |
||
12 |
26-Sep
|
Topic 10 Qualitative and Quantitative HPLC – Using standards to find what substances are in a sample and at what concentration |
Lab 10 1a. Prepare Tablets for HPLC 1b. HPLC of Tablets 2. TLC of aspirin, caffeine & paracetamol |
Quiz 4(HPLC) Logbook |
||
13 |
3-Oct |
Topic 11 HPLC Analgesics results Topic 12 Internal standards |
Lab 11 HPLC of analgesics interpreting results
|
Logbook due by 5pm on Thursday |
||
14 |
10-Oct |
Topic 13 GC theory & calculations
|
Lab 12 GC @ Applied Chemistry Gas Chromatography of Xylene |
Quiz 5 |
||
15 |
17-Oct |
Revision |
|
|||
16 |
24-Oct |
Exam Week |
Exam 2 |
Exam 2 |
||
17 |
31-Oct |
Alternate assessment (Special consideration only) |
|
|||
* Oral practical report = presentation to class at beginning of Thursday class
Subject to change
Learning Resources
Prescribed Texts
Perform Routine Chromatographic Techniques Practical Manual (available online) |
|
• J Crowe, T. Bradshaw, P. Monk (2006) |
References
Other Resources
Overview of Assessment
Assessment for this course may include laboratory reports, assignments, exams and practival assessment in the laboratory
Assessment Tasks
Assessments |
Week done |
% |
Assessment 1. Theory Online Quizzes
Week 3: TLC, Paper chroma Week 5: SEC, IEC Week 6: CSL Case study Week 9: AC, HIC, SPE Week 12: HPLC Week 14: GC Experiment
|
Weeks 3, 5, 6, 9, 12, 14 |
Must have an average pass grade for all quizzes
20%
|
Assessment 2. 2 Theory Exams Exam 1 Topics: Paper Chromatography, TLC, SEC, IEC, and multistep Chromatography Exam 2 Topics: HPLC & GC only |
Endof |
25%+ 25% |
Assessment 3. Laboratory practice. | ||
1: Paper Chromatography 2: HPLC of Caffeine in drinks spreadsheet assignment
|
Report 1: Week 3 – write up Report 2:
|
15%+ 15% |
At least 3 experiments must show Reliable and Valid*** results to pass this component. Acceptance criteria and Typical ranges are given in the lab manual or in class.
|
All lab sessions attended Submit completed logbook by Friday 5pm of week 14 |
Satisfactory |
|
Total |
100% |
Assessment Information
** see logbook information for what is required.
*** Reliable and Valid results: Reliable means that the results are within the typical range (ie reasonable results - the experiment has worked as expected. Valid means that the method uses validation checks and that the standards/controls results are within the acceptance criteria used to define validity (see lab manual). The validation checks are standards and/or controls.
Suitable methods to use for this purpose are indicated with “valid method” in the class schedule.
**Students must attend > 80% of practical classes AND pass all assessments to pass the course**.
- To pass the course you need to pass, on average, each type of assessment (exam, prac reports, and assignments etc.). For example, if there are two tests you need to have an average of 50% to pass. But you also need to have passed the other assessments as well. You can’t make up marks from one type of assessment to another (e.g. pass the tests but fail the prac reports).
- Extension of time for submission of assessable work- A student may apply for an extension of up to 7 days from the original due date. They must lodge the application form (available on the web http://mams.rmit.edu.au/seca86tti4g4z.pdf ) at least the day before the due date. The application is lodged with the School Admin Office on Level 6, Bdg 51. Students requiring longer extensions must apply for Special Consideration (form available on the Web). For missed assessments such as exams- you (& your doctor if you are sick) must fill out a special consideration form. This form must be lodged at the HUB or online with supporting evidence (eg medical certificate), prior to, or within, 48 hours of the scheduled time of examination.
- Late work that is submitted without an application for an extension will not be corrected.
- If you miss an assessment task due to unavoidable circumstances, you need to follow the procedure of special consideration and apply within the allowed time frame.
What does “all assessments must be passed” mean exactly?
It is your responsibility to understand exactly what you need to do to pass the course, but hopefully the information below will make things clear… if you need more information, please ask a teacher J
This means you need to pass, on average, each type of assessment listed in the table ie:
- Average exam mark (2 exams) is a pass,
- Average prac report mark (2 reports) is a pass,
- Average quiz mark (of 6 quizzes) is a pass,
- Logbook is passed, including >80% attendance.
If you miss a prac class you need to provide a copy of your medical certificate or other evidence (court appearance, funeral notice, statutory declaration) to the teacher.
Practical Reports
- If you miss a prac report class you will only be eligible to write up a different prac if you provide a medical certificate. For this to occur you need to discuss your situation with the teacher as soon as possible. Other reasons for missing a write up prac do not mean that you can write up a different report.
- Reports will be submitted online via blackboard at the link for each report.
Reports will be reviewed for plagiarism by Turnitin.
Reports found to contain an inappropriate amount of copied text will be required to be resubmitted within one week of notification by email. Students should aim to have less than 20% of text detected by Turnitin. If ‘proforma documents’ are provided to help you format your report, you should remove instructional text. - Please read the online anti-plagiarism statement given in blackboard submission of work via Blackboard incorporates student agreement to these conditions.
- Practical report 2 (a spreadsheet activity) is due in the analysis session.
Quizzes
- Each quiz has a published due date. Quizzes are to be done before the class in the same week to help you prepare for the class. Quizzes may include questions on safety.
- To pass the quizzes component you must get an average pass for all quizzes (ie. 50% average). The score for quizzes is calculated from the average grade of all quizzes. If you miss a quiz and have submitted a medical certificate you may do it later than the due date as long as you contact the teacher asap after your absence.
Students must attend at least 80% of practical classes.
- If you miss a prac class you should provide a copy of your medical certificate or other evidence (court appearance, funeral notice, statutory declaration) to the teacher.
Assessment Matrix
Other Information
This course is graded in accordance with competency-based assessment, but which also utilise graded assessment
CHD Competent with High Distinction (80 – 100%)
CDI Competent with Distinction (70 – 79%)
CC Competent with Credit (60 – 69%)
CAG Competency Achieved – Graded (50 – 59%)
NYC Not Yet Competent
DNS Did Not Submit for assessment
• To pass the course you need to pass, on average, each type of assessment (exams, pracs and assignments etc.) For example, if there are two exams you need to have an average of 50% to pass and you also need to have passed the other assessment types. You can’t make up marks from one type of assessment to another (e.g. pass the exams but fail the prac component).
• Late work that is submitted without an application for an extension (see below) will not be corrected.
• APPLICATION FOR EXTENSION OF TIME FOR SUBMISSION OF ASSESSABLE WORK - A student may apply for an extension of up to 7 days from the original due date. They must lodge the application form (available online http://www1.rmit.edu.au/students/assessment/extension) at least 24 hours before the due date. The application is lodged with the School Admin Office on Level 6, Bdg 51. Students requiring longer extensions must apply for SPECIAL CONSIDERATION.
• For missed assessments such as exams- you (& your doctor if you are sick) must fill out a special consideration form. This form must be lodged online with supporting evidence prior to, or within, 48 hours of the scheduled time of examination http://www1.rmit.edu.au/students/specialconsideration
Course Overview: Access Course Overview