Course Title: Perform tissue and cell culture techniques
Part B: Course Detail
Teaching Period: Term2 2013
Course Code: ONPS5664C
Course Title: Perform tissue and cell culture techniques
School: 155T Vocational Health and Sciences
Campus: City Campus
Program: C5282 - Diploma of Laboratory Technology (Biotechnology)
Course Contact: Amberlee Mitton
Course Contact Phone: +61 3 9925 8053
Course Contact Email: amber.mitton@rmit.edu.au
Name and Contact Details of All Other Relevant Staff
Amberlee Mitton
amberlee.mitton@rmit.edu.au
Nominal Hours: 100
Regardless of the mode of delivery, represent a guide to the relative teaching time and student effort required to successfully achieve a particular competency/module. This may include not only scheduled classes or workplace visits but also the amount of effort required to undertake, evaluate and complete all assessment requirements, including any non-classroom activities.
Pre-requisites and Co-requisites
The following 3 competencies are all pre-requisites:
MSL974006A Perform biological procedures
MSL973007A Perform microscopic examination
MSL973004A Perform aseptic techniques
Course Description
This unit of competency covers the ability to prepare, maintain and preserve cells and cell lines for a variety of applications, such as large scale culture, production of monoclonal antibodies, production of viral vaccines and amniocentesis studies. Personnel are required to optimise equipment setup, media and growth techniques. They are required to detect and investigate contamination and take preventative and/or corrective actions under supervision.
National Codes, Titles, Elements and Performance Criteria
National Element Code & Title: |
MSL975013A Perform tissue and cell culture techniques |
Element: |
1. Interpret and schedule production requirements |
Performance Criteria: |
1.1.Review client request and confirm quantity and |
Element: |
2. Work safely according to the legal and regulatory framework |
Performance Criteria: |
2.1.Ensure work practices and personal actions conform |
Element: |
3. Assemble and maintain tissue culture equipment |
Performance Criteria: |
3.1.Assemble, sterilise or decontaminate equipment |
Element: |
4. Prepare and test cell and tissue culture media |
Performance Criteria: |
4.1.Confirm media specifications and processes/methods |
Element: |
5. Obtain, monitor and maintain tissue and cell lines |
Performance Criteria: |
5.1.Retrieve/obtain the cell lines or tissue sample from 5.5.Monitor growth of tissue and cell lines and products |
Element: |
6. Preserve cells and tissues |
Performance Criteria: |
6.1.Select the appropriate preservation method |
Element: |
7. Maintain records |
Performance Criteria: |
7.1.Maintain records of batches of media and test data |
Learning Outcomes
Details of Learning Activities
Teacher directed delivery of lessons
• Class discussion
• Worksheets
• Laboratory experiments
• Record keeping of experiments
• Research activities
• Library sessions
Teaching Schedule
1. | Library session | Topic 1: Intro to animal tissue culture (work area & equipment) & Cell Biology: Cell structure and organelles |
Assessment 1: History of tissue culture research assignment DUE Date: Week 5 15% |
2. | PRAC1: Aseptic technique in the biohazard cabinet | Topic 2: Cell Biology: Life cycle, division and death | Logbook |
3. |
PRAC 2: Pipetting and transfer of fluids PRAC 3: Preparation of tissue culture solutions and sterility testing |
Topic 3: Culture environment - Media and growth requirements | Logbook |
4. | PRAC 4: Morphology of INT407 cells | Topic 4: The biology of cultured cells | Logbook |
5. | PRAC 5: Feeding INT407 cells | Topic 5: Cell types | Logbook |
6. | PRAC 6: Passaging INT407 cells using a split ratio | Topic 6: Cell growth | Logbook |
7. | - | Revision | |
8. | - | Mid-Semester Exams | Assessment 2: Mid semester exam (30%) |
9. | PRAC 7: Cell counting using the manual method and automatic coulter | Topic 7: Cell counts and viable cells | Assessment 3: Practical report including peer review and edit. Peer review and edit (C/NYC) Report 15% DUE Date: Week 11 |
10. | PRAC 8: Passaging a cell line using cell density | Topic 8: Contamination & Filtration, sterilization and disinfection | Logbook |
11. | PRAC 9: Effect of pH og cell growth | Topic 9: Cell preservation, storage and recovery | Logbook |
Student vacation | Student vacation | ||
12. | PRAC 10: Effect of seeding cell density on cell growth |
Topic 10: Tissue and cell culture in industry 1: Stem cells | Logbook |
13. | PRAC 11: Effect of FBS percentage on cell growth |
Topic 11: Tissue and cell culture in industry 2: Monoclonal antibodies | Logbook |
14. | Prac 12: Practical exam | - | Practical exam (C/NYC) |
15. | - | Revision | Logbook (C/NYC) |
16. | - | End of Semester Exam | Assessment 4: End of Semester Exam 30% |
Learning Resources
Prescribed Texts
References
Other Resources
Overview of Assessment
Assessments for this course typically consist of:
• Class activities
• Assignments
• Presentations
• Exams
Assessment Tasks
- Assessment 1: History of tissue culture research assignment 15% DUE Date: Week 5
- Assessment 2: Mid semester exam 30%
- Assessment 3: Practical report including peer review and edit. Peer review and edit (C/NYC) Report 15% DUE Date: Week 11
- Assessment 4: End of Semester Exam 30%
- Practical Exam (C/NYC)
- Log book (C/NYC)
Assessment Matrix
Other Information
Assessment Information
• To pass the course you need to pass, on average, each type of assessment (exam, prac reports, and assignments etc.). For example, if there are two tests you need to have an average of 50% to pass. But you also need to have passed the other assessments as well. You can’t make up marks from one type of assessment to another (eg pass the tests but fail the prac reports).
• Late work that is submitted without an application for an extension will not be corrected.
• Extension of time for submission of assessable work- A student may apply for an extension of up to 7 days from the original due date. They must lodge the application form (available on the web http://mams.rmit.edu.au/seca86tti4g4z.pdf) at least the day before the due date. The application is lodged with the School Admin Office on Level 6, Bdg 51. Students requiring longer extensions must apply for Special Consideration (form available on the Web). For missed assessments such as exams- you (& your doctor if you are sick) must fill out a special consideration form. This form must be lodged at the HUB or online with supporting evidence (eg medical certificate), prior to, or within, 48 hours of the scheduled time of examination.
• In order to be successful in this module, you have to achieve a pass in all of the above assessment tasks. If you miss an assessment task due to unavoidable circumstances, you need to follow the procedure of special consideration and apply within the allowed time frame.
If you have any questions about this assessment process, feel free to email me on amberlee.mitton@rmit.edu.au
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