Course Title: Perform tissue and cell culture techniques
Part B: Course Detail
Teaching Period: Term2 2015
Course Code: ONPS5664C
Course Title: Perform tissue and cell culture techniques
School: 155T Vocational Health and Sciences
Campus: City Campus
Program: C5282 - Diploma of Laboratory Technology (Biotechnology)
Course Contact: Amberlee Mitton
Course Contact Phone: +61 3 9925 8053
Course Contact Email: amber.mitton@rmit.edu.au
Name and Contact Details of All Other Relevant Staff
Muriel Aldunate
Nominal Hours: 100
Regardless of the mode of delivery, represent a guide to the relative teaching time and student effort required to successfully achieve a particular competency/module. This may include not only scheduled classes or workplace visits but also the amount of effort required to undertake, evaluate and complete all assessment requirements, including any non-classroom activities.
Pre-requisites and Co-requisites
The following 3 competencies are all pre-requisites:
MSL974006A Perform biological procedures
MSL973007A Perform microscopic examination
MSL973004A Perform aseptic techniques
Course Description
This unit of competency covers the ability to prepare, maintain and preserve cells and cell lines for a variety of applications, such as large scale culture, production of monoclonal antibodies, production of viral vaccines and amniocentesis studies. Personnel are required to optimise equipment setup, media and growth techniques. They are required to detect and investigate contamination and take preventative and/or corrective actions under supervision.
National Codes, Titles, Elements and Performance Criteria
National Element Code & Title: |
MSL975013A Perform tissue and cell culture techniques |
Element: |
1. Interpret and schedule production requirements |
Performance Criteria: |
1.1. Review client request and confirm quantity and nature of cells, tissue or products |
Element: |
2. Work safely according to the legal and regulatory framework |
Performance Criteria: |
2.1. Ensure work practices and personal actions conform to regulations, codes, guidelines and enterprise quality assurance procedures |
Element: |
3. Assemble and maintain tissue culture equipment |
Performance Criteria: |
3.1. Assemble, sterilise or decontaminate equipment according to enterprise procedures |
Element: |
4. Prepare and test cell and tissue culture media |
Performance Criteria: |
4.1. Confirm media specifications and processes/methods |
Element: |
5. Obtain, monitor and maintain tissue and cell lines |
Performance Criteria: |
5.1. Retrieve/obtain the cell lines or tissue sample from fresh or preserved sources and prepare a culture |
Element: |
6. Preserve cells and tissues |
Performance Criteria: |
6.1. Select the appropriate preservation method |
Element: |
7. Maintain records |
Performance Criteria: |
7.1. Maintain records of batches of media and test data |
Learning Outcomes
Details of Learning Activities
Teacher directed delivery of lessons
• Class discussion
• Worksheets
• Laboratory experiments
• Record keeping of experiments
• Research activities
Teaching Schedule
1. | HeLa background and info session for Assessment 1 | Topic 1: Intro to animal tissue culture (work area & equipment) & Cell Biology: Cell structure and organelles |
Assessment 1: History of tissue culture research assignment DUE Date: Week 4 15% |
2. | PRAC 1: The tissue culture laboratory and instruments
PRAC 2: Aseptic technique in the biohazard cabinet |
Topic 2: Cell Biology: Life cycle, division and death | Log book |
3. |
PRAC 3: Pipetting and transfer of fluids PRAC 4: Preparation of tissue culture solutions and sterility testing |
Topic 3: Culture environment - Media and growth requirements | Logbook |
4. |
PRAC 5: Morphology of INT407 cells PRAC 6: Feeding INT407 cells |
Topic 4: The biology of cultured cells | Logbook |
5. | PRAC 7: Passaging INT407 cells using a split ratio | Topic 5: Cell types | Logbook |
6. | PRAC 8: Cell counting using the manual method and automatic coulter GROUP A | Topic 6: Cell growth | Assessment 2: Practical report including peer review and edit. Peer review and edit (S/NYS) Report 20% DUE Date: Week 10 & 11 |
7. | PRAC 8: Cell counting using the manual method and automatic coulter GROUP B | Revision | Assessment 2: Practical report including peer review and edit. Peer review and edit (S/NYS) Report 20% DUE Date: Week 10 & 11 |
8. | Exam week | Exam week | Assessment 3: Mid semester exam (25%) |
Student vacation | |||
9. | PRAC 9: Passaging a cell line using cell density |
Topic 7: Cell counts and viable cells | Log book |
10. | Assessment 3 – In class peer review and edit – this class is compulsory
Classroom - TBA |
Topic 8: Contamination | |
11. | PRAC 10 - Cell preservation, storage and recovery | Topic 9: Cell preservation, storage and recovery | Logbook |
12. | PRAC 11 - Repeat of PRAC 7: Passaging INT407 cells using a split ratio GROUP A |
Topic 10: Filtration, sterilization and disinfection | Logbook |
13. | PRAC 11 - Repeat of PRAC 7: Passaging INT407 cells using a split ratio GROUP B |
Topic 11: Tissue and cell culture in industry 1: Stem cells | Logbook |
14. | Log book grading and feedback | Topic 12: Tissue and cell culture in industry 2: Monoclonal antibodies | Practical exam (S/NYS) |
15. | Practical exam | Revision | Assessment 4: Logbook (15%) |
16. | - | End of Semester Exam | Assessment 5: End of Semester Exam 25% |
Learning Resources
Prescribed Texts
References
Other Resources
Overview of Assessment
Assessments for this course typically consist of:
• Class activities
• Assignments
• Presentations
• Exams
Assessment Tasks
- Assessment 1: History of tissue culture research assignment 15% DUE Date: Week 4
- Assessment 2: Practical report including peer review and edit. Peer review and edit (C/NYC) Report 20% DUE Date: Week 10 & 11
- Assessment 3: Mid semester exam 25%
- Assessment 4: Log book (15%) Due week 14
- Assessment 5: End of Semester Exam 25%
- Practical Exam (S/NYS)
Assessment Matrix
Other Information
Assessment Information
• This course is graded in accordance with competency-based assessment, but which also utilise graded assessment
CHD Competent with High Distinction (80 – 100%)
CDI Competent with Distinction (70 – 79%)
CC Competent with Credit (60 – 69%)
CAG Competency Achieved – Graded (50 – 59%)
NYC Not Yet Competent
DNS Did Not Submit for assessment
• To pass the course you need to pass, on average, each type of assessment (exams, pracs, and assignments etc.) For example, if there are two exams you need to have an average of 50% to pass and you also need to have passed the other assessment types. You can’t make up marks from one type of assessment to another (e.g. pass the exams but fail the prac component).
• Late work that is submitted without an application for an extension (see below) will not be corrected.
• APPLICATION FOR EXTENSION OF TIME FOR SUBMISSION OF ASSESSABLE WORK - A student may apply for an extension of up to 7 days from the original due date. They must lodge the application form (available online http://www1.rmit.edu.au/students/assessment/extension) at least 24 hours before the due date. The application is lodged with the School Admin Office on Level 6, Bdg 51. Students requiring longer extensions must apply for SPECIAL CONSIDERATION.
For missed assessments such as exams- you (& your doctor if you are sick) must fill out a special consideration form. This form must be lodged online with supporting evidence prior to, or within, 48 hours of the scheduled time of examination http://www1.rmit.edu.au/students/specialconsideration
Course Overview: Access Course Overview