Course Title: Perform tissue and cell culture techniques
Part B: Course Detail
Teaching Period: Term1 2016
Course Code: ONPS5664C
Course Title: Perform tissue and cell culture techniques
School: 155T Vocational Health and Sciences
Campus: City Campus
Program: C5282 - Diploma of Laboratory Technology (Biotechnology)
Course Contact: Amberlee Mitton
Course Contact Phone: +61 3 9925 8053
Course Contact Email: amber.mitton@rmit.edu.au
Name and Contact Details of All Other Relevant Staff
Muriel Aldunate
Nominal Hours: 100
Regardless of the mode of delivery, represent a guide to the relative teaching time and student effort required to successfully achieve a particular competency/module. This may include not only scheduled classes or workplace visits but also the amount of effort required to undertake, evaluate and complete all assessment requirements, including any non-classroom activities.
Pre-requisites and Co-requisites
The following 3 competencies are all pre-requisites:
MSL974006A Perform biological procedures
MSL973007A Perform microscopic examination
MSL973004A Perform aseptic techniques
Course Description
This unit of competency covers the ability to prepare, maintain and preserve cells and cell lines for a variety of applications, such as large scale culture, production of monoclonal antibodies, production of viral vaccines and amniocentesis studies. Personnel are required to optimise equipment setup, media and growth techniques. They are required to detect and investigate contamination and take preventative and/or corrective actions under supervision.
National Codes, Titles, Elements and Performance Criteria
National Element Code & Title: |
MSL975013A Perform tissue and cell culture techniques |
Element: |
1. Interpret and schedule production requirements |
Performance Criteria: |
1.1. Review client request and confirm quantity and nature of cells, tissue or products |
Element: |
2. Work safely according to the legal and regulatory framework |
Performance Criteria: |
2.1. Ensure work practices and personal actions conform to regulations, codes, guidelines and enterprise quality assurance procedures |
Element: |
3. Assemble and maintain tissue culture equipment |
Performance Criteria: |
3.1. Assemble, sterilise or decontaminate equipment according to enterprise procedures |
Element: |
4. Prepare and test cell and tissue culture media |
Performance Criteria: |
4.1. Confirm media specifications and processes/methods |
Element: |
5. Obtain, monitor and maintain tissue and cell lines |
Performance Criteria: |
5.1. Retrieve/obtain the cell lines or tissue sample from fresh or preserved sources and prepare a culture |
Element: |
6. Preserve cells and tissues |
Performance Criteria: |
6.1. Select the appropriate preservation method |
Element: |
7. Maintain records |
Performance Criteria: |
7.1. Maintain records of batches of media and test data |
Learning Outcomes
Details of Learning Activities
Teacher directed delivery of lessons
• Class discussion
- Worksheets
- Laboratory experiments
- Record keeping of experiments
- Research activities
- group work
Teaching Schedule
1. | 8/2 | TOPIC 1 - Intro to biohazard cabinets, aseptic technique and tissue culture TOPIC 2 - Intro to plant tissue culture and plant physiology | PRAC 1 : Introduction to the Biohazard hood, aseptic technique and tissue culture PRAC 2: Media preparation | Log book |
2. | 15/2 | TOPIC 3 – Plant growth |
PRAC 3: Seed (Lepidium sativum) propagation PRAC 4: Surface sterilisation preparation | Log book |
3. | 22/2 |
TOPIC 4 – Plant reproduction
|
PRAC 5: Lepidium sativum seedling transfer PRAC 6: Surface sterilisation and exp |
Logbook |
4. | 29/2 | TOPIC 5 - Plant tissue culture environment |
PRAC 7: Effect of surface sterilisation and explant of Petunia leaves & Collect results from PRAC 6 | Logbook |
5. | 7/3 | TOPIC 6 – Media and growth regulators | PRAC 8: Collect results from PRAC 7 and compare results from PRAC 6 &7 Discussion of PRAC results and Assessment 1 | Assessment 1 provided: Prac report (PRAC 5, 6, 7 & 8) DUE: Week 9 |
6. | 14/3 | LABOUR DAY PUBLIC HOLIDAY | NO PRAC | |
7. | 21/3 | TOPIC 7 – Micro propagation 1 & 2 | PRAC 9: Cauliflower explant | Log book |
28/3 | Student vacation | NO PRAC | ||
8. | 4/4 | Exam week | Exam week | |
9. | 11/4 | TOPIC 9 – Contamination TOPIC 10 – GMO’s |
PRAC 10: Carrot callus culture | Assessment 2 provided: GMO group presentation DUE Week 13 |
10. | 18/4 |
|
PRAC 11: Succulent explant | Assessment 2: DRAFT DUE |
11. | 25/4 | ANZAC DAY PUBLIC HOLIDAY | NO PRAC Assessment 2 preparation |
Log book |
12. | 2/5 |
|
PRAC 12: Collect PRAC 11 results and take plants home collect PRAC 10 results (3 weeks post explant) |
Log book |
13. | 9/5 | |
GROUP PRESENTATIONS | PRESENTATIONS DUE |
14. | 16/5 | Revision | Log book check and feedback | Assessment 3: Log Book – Competency based assessment (S/NYS) (Observational and note keeping skills) |
15. | 23/5 | Practical exam (Explant) | Assessment 4: Practical Exam – Competency based assessment (S/NYS) Technical skills | |
16. | - | End of Semester Exam | Assessment 5: End of year quiz |
teaching schedule for Semester 2 to follow
Learning Resources
Prescribed Texts
References
Other Resources
Overview of Assessment
Assessments for this course typically consist of:
• Class activities
• Assignments
• Presentations
• Exams
Assessment Tasks
Semester 1
- Assessment 1: Prac report. 30% Due week 9
- Assessment 2: GMO group presentation 30% Due Week 13
- Assessment 3: Log book (S/NYS) Due week 15
- Assessment 4: Skills test (S/NYS) week 14
- Assessment 5: Written test 40% week 16
Semester 2
- Assessment 1: History of tissue culture research assignment 15% DUE Date: Week 4
- Assessment 2: Practical report including peer review and edit. Peer review and edit (C/NYC) Report 20% DUE Date: Week 10 & 11
- Assessment 3: Mid semester exam 25%
- Assessment 4: Log book (15%) Due week 14
- Assessment 5: End of Semester Exam 25%
- Practical Exam (S/NYS)
Assessment Matrix
Other Information
Assessment Information
• This course is graded in accordance with competency-based assessment, but which also utilise graded assessment
CHD Competent with High Distinction (80 – 100%)
CDI Competent with Distinction (70 – 79%)
CC Competent with Credit (60 – 69%)
CAG Competency Achieved – Graded (50 – 59%)
NYC Not Yet Competent
DNS Did Not Submit for assessment
• To pass the course you need to pass, on average, each type of assessment (exams, pracs, and assignments etc.) For example, if there are two exams you need to have an average of 50% to pass and you also need to have passed the other assessment types. You can’t make up marks from one type of assessment to another (e.g. pass the exams but fail the prac component).
• Late work that is submitted without an application for an extension (see below) will not be corrected.
• APPLICATION FOR EXTENSION OF TIME FOR SUBMISSION OF ASSESSABLE WORK - A student may apply for an extension of up to 7 days from the original due date. They must lodge the application form (available online http://www1.rmit.edu.au/students/assessment/extension) at least 24 hours before the due date. The application is lodged with the School Admin Office on Level 6, Bdg 51. Students requiring longer extensions must apply for SPECIAL CONSIDERATION.
For missed assessments such as exams- you (& your doctor if you are sick) must fill out a special consideration form. This form must be lodged online with supporting evidence prior to, or within, 48 hours of the scheduled time of examination http://www1.rmit.edu.au/students/specialconsideration
Course Overview: Access Course Overview