Course Title: Perform histological tests
Part B: Course Detail
Teaching Period: Term1 2018
Course Code: BIOL5224C
Course Title: Perform histological tests
School: 174T School of VE Engineering, Health & Science
Campus: City Campus
Program: C5362 - Diploma of Laboratory Technology (Pathology Testing)
Course Contact: Namrita Kaul
Course Contact Phone: +61 3 9925 4837
Course Contact Email: namrita.kaul@rmit.edu.au
Name and Contact Details of All Other Relevant Staff
Rowena Lavery
rowena.lavery@rmit.edu.au
Nominal Hours: 150
Regardless of the mode of delivery, represent a guide to the relative teaching time and student effort required to successfully achieve a particular competency/module. This may include not only scheduled classes or workplace visits but also the amount of effort required to undertake, evaluate and complete all assessment requirements, including any non-classroom activities.
Pre-requisites and Co-requisites
Pre-requisites:
MSL973007 Perform microscopic examination
Course Description
This unit of competency covers the ability to perform tests and procedures associated with processing and staining tissues for examination of tissue structure and abnormalities by pathologists and scientists to assist with disease diagnosis. The unit covers tests and procedures that are associated with anatomical pathology and may involve the use of automated processors and staining machines.
National Codes, Titles, Elements and Performance Criteria
National Element Code & Title: |
MSL975003 Perform histological tests |
Element: |
1 Process specimens and associated request forms |
Performance Criteria: |
1.1 Check and match specimens and request forms before they are accepted |
Element: |
2 Prepare specimens for cut-up |
Performance Criteria: |
2.1 Arrange tissues and request forms in cut-up area |
Element: |
3 Process tissue |
Performance Criteria: |
3.1 Select processor program and reagents |
Element: |
4 Embed tissue |
Performance Criteria: |
4.1 Check that temperature of embedding medium is suitable for embedding process |
Element: |
5 Cut tissue sections |
Performance Criteria: |
5.1 Check that flotation bath is ready and satisfactory for use |
Element: |
6 Stain tissue sections |
Performance Criteria: |
6.1 Apply staining procedures to demonstrate required morphological features |
Element: |
7 Maintain a safe environment |
Performance Criteria: |
7.1 Use established safe work practices and personal protective equipment (PPE) to ensure personal safety and that of other laboratory personnel |
Element: |
8 Maintain laboratory records |
Performance Criteria: |
8.1 Make entries on report forms or into computer systems, accurately calculating, recording or transcribing data as required |
Learning Outcomes
Details of Learning Activities
learning activities include
face-to-face theory classes
practical experiments & techniques
online activities
Teaching Schedule
Semester 1
Week | Date starting | Theory Topic | Practical Activities | Assessment |
1 | 5/2 | No scheduled class | ||
2 | 12/2 | No scheduled class | ||
3 | 19/2 | Introduction to histology, organising a histology laboratory |
Tissue collection and fixation (rat dissection) Tissue processing – samples sent to Bundoora |
|
4 | 26/2 | Embedding
Tissue accessioning, Specimen reception, Tissue processing – manual and automatic |
Specimen reception- matching samples and request for 10 samples | |
5 | 5/3 | Microtomy – introduction | Grp A-embedding of samples Grp B- microtomy on kidney (10 slides) |
Assessment 1. OBSERVATIONAL CHECKLIST- tissue processing and embedding Assessment 2. OBSERVATIONAL CHECKLIST- microtomy |
6 | 12/3 | Microtomy and frozen sections (cryotomy and the use of cryostat). Tissue processing troubleshooting and possible effects on microtomy. |
Grp A- microtomy on kidney (10 slides) Grp B- embedding of samples & specimen reception- matching samples and request for 10 samples |
|
7 | 19/3 | Histological images of epithelial and connective tissue | Grp A - embedding & specimen reception- matching samples and request for 10 samples |
|
26/3 | Mid semester break | |||
8 | 2/4 | Exam week | Assessment 3. SIMULATED LIMS ASSESSMENT (Identify non-compliant specimen request forms and log acceptable specimens into LIMs) | |
9 | 9/4 | Histological images of adipose tissue and blood vessels | Grp A- Microtomy on liver (14 slides) Grp B- embedding & specimen reception- matching samples and request for 10 samples |
|
10 | 16/4 | Bone and biopsies processing | Bone collection- decalcification biopsy collection (Kidney core needle biopsy) |
|
11 | 23/4 | Histological images of nerve tissue | Bone fixation and manual tissue processing of biopsies |
|
12 | 30/4 | Histological images of muscular tissue (skeletal and cardiac) | Grp A- OHS and preparation of reagents exercises Grp B- Microtomy on lung (6 slides) |
|
13 | 7/5 | Histological images of upper and lower digestive system | ||
14 | 14/5 | Histological images of upper and lower digestive system | Grp A- Microtomy on skin (10 slides) Grp B- Staining H & E (occasion #1) |
|
15 | 21/5 | Assessment 4. END OF SEMESTER 1 PRACTICAL EXAM – prepare specimens for cut up | ||
16 | 28/5 | Assessment 5. END OF SEMESTER 1 THEORY EXAM - Online Quiz 1: tissue processing, embedding, microtomy |
Semester 2
Week | Date starting |
Theory topic | Practical activities | Assessment |
1 | 2/7 | Histological images of Haematopoietic tissues |
Grp A- Staining H & E (occasion #1) Grp B- Microtomy on skin (10 slides) |
Assessment 6. OBSERVATIONAL CHECKLIST – staining |
2 | 9/7 | Introduction to staining | Grp A- Microtomy on tongue (7 slides) Grp B- Staining H & E (occasion # 2) |
|
3 | 16/7 | Routine Haematoxylin & Eosin staining (H &E) |
Grp A- Staining H & E (occasion # 2) Grp B- Microtomy on tongue (7 slides) |
|
4 | 23/7 | Pigments | Grp A- Microtomy on tongue (7 slides) Grp B- Staining H & E (occasion # 3) |
|
5 | 30/7 | Connective tissue stains | Grp A- Staining H & E (occasion # 3) Grp B- Microtomy on tongue (7 slides) |
|
6 | 6/8 | Staining for Carbohydrates | Grp A - Microtomy: Catch up Grp B- Staining PAS (Kidney section X1) occasion # 1 Staining PTAH (tongue section x1) occasion # 1 |
|
7 | 13/8 | Staining for Mucins | Grp A- Staining PAS (Kidney section X1) occasion # 1 Staining PTAH (tongue section x1) occasion # 1 Grp B- Microtomy: Catch up |
|
8 | 20/8 | Assessment 7:Online Quiz 2 general staining knowledge | ||
27/8 | Mid semester break | |||
9 | 3/9 | Histological images of respiratory system | Perls staining for iron deposits (Liver x1 ) occasion # 1 Masson staining for connective tissue (Skin x 1) occasion # 1 Take photos of stained slides for portfolio: H & E, PAS, PTAH, Perls, Masson |
|
10 | 10/9 | Histological images of urinary system | Perls staining for iron deposits (Liver x1 ) occasion # 2 Masson staining for connective tissue (Skin x 1) occasion # 2 Take photos of stained slides for portfolio: H & E, PAS, PTAH, Perls, Masson |
|
11 | 17/9 | Histological images of male and female reproductive system | PASD staining for carbohydrates (Liver x1 ) occasion # 1 ABPAS staining for Mucins (Tongue x 1) occasion # 1 Take photos of stained slides for portfolio: H & E, PAS, PTAH, Perls, Masson etc |
|
12 | 24/9 | Polarising Microscopy | PASD staining for carbohydrates (Liver x1 ) occasion # 2 ABPAS staining for Mucins (Tongue x 1) occasion # 2 Take photos of stained slides: H & E, PAS, PTAH, Perls, Masson, PASD, ABPAS |
|
13 | 1/10 | Catch up staining Archive Box: Labelled stained slides to be archived, record slide location in your portfolio. |
||
14 | 8/10 | Catch up staining Archive Box: Labelled stained slides to be archived, record slide location in your portfolio. |
||
15 | 15/10 | Assessment 8: PORTFOLIO SUBMISSION, slides | ||
16 | 22/10 | Assessment 9: Online Quiz 3 IHC, Polarising Microscope. |
Learning Resources
Prescribed Texts
References
Other Resources
Overview of Assessment
written tests
practical tests
Laboratory logbook
online quizzes
Assessment Tasks
Assessment | Date | Result | Grades allocated |
1. OBSERVATIONAL CHECKLIST- tissue processing and embedding | 8/03- 10/05 | S/NYS | |
2. OBSERVATIONAL CHECKLIST- microtomy | 8/3- 2/8 | S/NYS | |
3. SIMULATED LIMS ASSESSMENT (Identify non-compliant specimen request forms and log acceptable specimens into LIMs) |
Week 8 & 9 | 15 | |
4. END OF SEMESTER 1 PRACTICAL EXAM – prepare specimens for cut up | 24/05/2018 | S/NYS | |
5. END OF SEMESTER 1 THEORY EXAM - Online Quiz 1: tissue processing, embedding, microtomy |
Week 16/17 | 15 | |
6. OBSERVATIONAL CHECKLIST – staining | 17/05 – 27/09 | S/NYS | |
7. MID-SEMESTER 2 THEORY EXAM – Online Quiz 2 general staining knowledge |
Week 8 (Sem 2) | 15 | |
8. END OF SEMESTER PORTFOLIO SUBMISSION | 18/10 | S/NYS | 40 |
9. END OF SEMESTER 2 THEORY EXAM – Online Quiz 3 IHC, Polarising Microscope. |
Week 16/17 | 15 |
Each task must be completed satisfactorily for competence to be achieved.
Competence must be achieved before grades are allocated.
Details about the requirements for the portfolio will be provided.
Assessment Matrix
Other Information
Assessment Information
This course is graded in accordance with competency-based assessment, but which also utilise graded assessment
CHD Competent with High Distinction (80 – 100%)
CDI Competent with Distinction (70 – 79%)
CC Competent with Credit (60 – 69%)
CAG Competency Achieved – Graded (50 – 59%)
NYC Not Yet Competent (0 – 49%)
DNS Did Not Submit for assessment
To pass the course you need to pass, on average, each type of assessment (exams, pracs, and assignments etc.) For example, if there are two exams you need to have an average of 50% to pass and you also need to have passed the other assessment types. You can’t make up marks from one type of assessment to another (e.g. pass the exams but fail the prac component). Late work that is submitted without an application for an extension (see below) will not be corrected.
APPLICATION FOR EXTENSION OF TIME FOR SUBMISSION OF ASSESSABLE WORK - A student may apply for an extension of up to 7 days from the original due date. They must lodge the application form (available online http://www1.rmit.edu.au/students/assessment/extension) at least 24 hours before the due date. The application form should be emailed to the Program Coordinator (namrita.kaul@rmit.edu.au) Students requiring longer extensions must apply for SPECIAL CONSIDERATION.
For missed assessments such as exams- you (& your doctor if you are sick) must fill out a special consideration form. This form must be lodged online with supporting evidence prior to, or within, 48 hours of the scheduled time of examination http://www1.rmit.edu.au/students/specialconsideration A minimum of 80% attendance at practical classes is required to pass this course. If you are sick you should submit a medical certificate to 6th floor office addressed to the Program Coordinator. If you miss a practical class that is a prac report write up you need to provide a medical certificate (or other evidence) and arrange an alternative report with your teacher
Plagiarism is the presentation of the work, idea or creation of another person as though it is your own. It is a form of cheating and is a very serious academic offence that may lead to expulsion from the University. Plagiarised material can be drawn from, and presented in, written, graphic and visual form, including electronic data and oral presentation. Plagiarism occurs when the origin of the material used is not appropriately cited. It also occurs through enabling plagiarism, which is the act of assisting or allowing another person to plagiarise or to copy your own work. Please make sure you consider this carefully in completing all your work and assessments in this course and if you are unsure about whether you might have plagiarised, seek help from your teacher.
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