Course Title: Perform aseptic techniques

Part B: Course Detail

Teaching Period: Term1 2017

Course Code: BIOL5227C

Course Title: Perform aseptic techniques

School: 174T School of VE Engineering, Health & Science

Campus: City Campus

Program: C5362 - Diploma of Laboratory Technology (Pathology Testing)

Course Contact: Amber Mitton

Course Contact Phone: +61 3 9925 8053

Course Contact Email:

Name and Contact Details of All Other Relevant Staff

Andrea McAdam

Nominal Hours: 40

Regardless of the mode of delivery, represent a guide to the relative teaching time and student effort required to successfully achieve a particular competency/module. This may include not only scheduled classes or workplace visits but also the amount of effort required to undertake, evaluate and complete all assessment requirements, including any non-classroom activities.

Pre-requisites and Co-requisites


Course Description

This unit of competency covers the ability to perform aseptic techniques to maintain the integrity of both the sample source and the sample. It applies to sampling techniques in tissue culture and to generic microbiological procedures.

National Codes, Titles, Elements and Performance Criteria

National Element Code & Title:

MSL973004 Perform aseptic techniques


1 Prepare for aseptic sampling or transfer

Performance Criteria:

1.1 Ensure that any sampling procedure conforms with the requirements of the sampling plan
1.2 Use specified personal protective equipment (PPE)
1.3 Prepare the work area for safe and effective sample transfer
1.4 Select equipment and materials specified by the procedure
1.5 Organise equipment to minimise contamination during manipulations
1.6 Label containers for clear identification
1.7 Record details in relevant log or database


2 Transfer materials aseptically

Performance Criteria:

2.1 Protect the integrity of the sample source by sterilising the sampling site and the mouth of transport or culture vessel
2.2 Sterilise inoculating loops and/or pipette where used to prevent contamination
2.3 Perform transfer while minimising opportunities for contamination and cross-infection
2.4 After transfer, and before sealing the transport or culture vessel, flame the vessel mouth to maintain sterility
2.5 Re-sterilise inoculating loops, minimising the generation of aerosols
2.6 Perform quality control checks as required 2.7 Label transport or culture vessels for clear identification


3 Maintain work area and equipment to prevent cross-infection and contamination

Performance Criteria:

3.1 Place disposable and reusable items into relevant receptacles
3.2 Clean and disinfect work area and equipment after use
3.3 Transport disposable and reusable contaminated materials to relevant areas for disinfection, sterilisation and cleaning or disposal

Learning Outcomes

Details of Learning Activities

Practical techniques performed in the laboratory

Online information in Blackboard

Additional activities for out-of-class individual study and investigation

Teaching Schedule

Week 8   Tue 28 Mar 

A1-1 Introduction to aseptic technique
A1-2 Patient specimens

Introduction to aseptic technique
Hand washing / Environmental swab / Lawn culture

Week 9   Tue 04 April 

Introduction to aseptic technique
A1-2 Patient specimens

Make media, Autoclave
Streak for single colonies
Review plates from Week 8 A1

Week 10 Mid-semester break

Week 11 ANZAC Day Tues 25 Apr 


Week 12   Tue 02 May 


A3-2 Disinfection

A3-3 Cross-contamination

Effectiveness of disinfection
Plating from swabs on bench
Review plates from Week 9 A2

Week 13   Tue 09 May 

Reading plates
A4-2 Gram staining

Plating from swabs
Gram stains / oil immersion
Review plates from Week 12 A3

Week 14   Tue 16 May 

A5-1 Antibiotic sensitivities
A5-2 Biosafety cabinets


Antibiotic sensitivity
Plating in Biosafety cabinet
Review plates from Week 13 A4

Week 15   Tue 23 May 

A6-1 Revision

Review plates from Week 14 A5
Practical Assessment

Week 16
END SEMESTER EXAMS – Written assessment

Learning Resources

Prescribed Texts


Other Resources

Overview of Assessment

practical skills assessment

laboratory logbook

online quizzes


Assessment Tasks

Logbook record of practical classes and post-class exercises (S/NYS).
At least five out of six practical classes must be completed.

Practical assessment: streak an agar plate aseptically for single colonies (S/NYS)

Written test (S/NYS) 75% pass considered satisfactory.

All assessment types must be completed to a satisfactory standard to pass this course.

Assessment Matrix

Other Information

This course is an ungraded competency.  It is a Pass only outcome – no higher grading. Outcome will be one of the following:

CA- Competency Achieved
NYC- Not Yet Competent
DNS- Did Not Submit for assessment

  • All assessment types must be passed (exams, prac, and assignments etc.).
    You can’t make up marks from one type of assessment to another (e.g. pass the tests but fail the prac component).
  • Attendance at practical classes is compulsory. A minimum of 80% attendance is required to pass this course. That is, 5 out of 6 practical classes.  If you are sick you should submit a medical certificate to 6th floor office addressed to the Program Coordinator
  • Late work that is submitted without an application for an extension will not be corrected.
  • APPLICATION FOR EXTENSION OF TIME FOR SUBMISSION OF ASSESSABLE WORK- A student may apply for an extension of up to 7 days from the original due date. They must lodge the application form (available online at least 24 hours before the due date. The application is lodged with the School Admin Office on Level 6, Bdg 51, or emailed to the Coordinator (

  • Students requiring extensions longer than 7 days must apply for Special Consideration (see the 'Help me' link in Blackboard via myRMIT studies or For missed assessments such as exams, you (and if you are sick, your doctor) must complete a Special Consideration form and submit it online with supporting evidence (eg medical certificate) before, or within 48 hours of the scheduled assessment.

Course Overview: Access Course Overview