Course Title: Perform immunohaematological tests

Part B: Course Detail

Teaching Period: Term2 2019

Course Code: BIOL5226C

Course Title: Perform immunohaematological tests

School: 174T School of VE Engineering, Health & Science

Campus: City Campus

Program: C5362 - Diploma of Laboratory Technology (Pathology Testing)

Course Contact: Amber Mitton

Course Contact Phone: +61 3 9925 8053

Course Contact Email:

Name and Contact Details of All Other Relevant Staff

Andrea McAdam

Nominal Hours: 80

Regardless of the mode of delivery, represent a guide to the relative teaching time and student effort required to successfully achieve a particular competency/module. This may include not only scheduled classes or workplace visits but also the amount of effort required to undertake, evaluate and complete all assessment requirements, including any non-classroom activities.

Pre-requisites and Co-requisites


MSL974006 Perform biological procedures

Course Description


This unit of competency covers the ability of technical personnel to perform routine tests and procedures that are part of the requirements of pre- and post-blood transfusion practice. The unit also covers tests and procedures that are indicated in laboratory investigations in obstetric and perinatal medicine, in suspected haemolysis and haemolytic episodes, and in other clinical circumstances.

National Codes, Titles, Elements and Performance Criteria

National Element Code & Title:

MSL975006 Perform immunohaematological tests


1 Process samples and associated request forms

Performance Criteria:

1.1. Check and match samples and request forms before they are accepted 1.2. Return samples and request forms that do not comply with requirements to their source with reasons for non-acceptance 1.3. Log acceptable samples, applying required document tracking mechanisms 1.4. Process samples as required by requested tests 1.5. Store sample components appropriately until required for testing


2 Perform tests

Performance Criteria:

2.1. Select authorised tests that are indicated for the requested investigations 2.2. Conduct individual tests according to documented methodologies, applying required quality control procedures 2.3. Record all results, noting any phenomena that may be relevant to the interpretation of results 2.4. Seek advice of section head or other responsible colleague when result interpretation is outside parameters of authorised approval 2.5. Store unused samples, for possible future reference, under conditions suitable to maintain viability


3 Maintain a safe environment

Performance Criteria:

3.1. Use established work practices and personal protective equipment to ensure personal safety and that of other laboratory personnel 3.2. Clean up spills using appropriate techniques to protect personnel, work area and environment from contamination 3.3. Minimise the generation of wastes 3.4. Ensure the safe disposal of biohazardous materials and other laboratory wastes in accordance with enterprise procedures


4 Maintain laboratory records

Performance Criteria:

4.1. Make entries on report forms or into computer systems, accurately recording or transcribing required data as required 4.2. Maintain instrument logs as required by accreditation checklists 4.3. Maintain records of blood and blood products received, used and returned to supplier 4.4. Maintain security and confidentiality of all clinical information, laboratory data and records


5 Issue blood and blood products

Performance Criteria:

5.1. Complete documentation required to permit the issuing of blood or blood components that have been cleared for use by clinical staff 5.2. Advise courier of transport requirements to ensure blood or blood products are delivered in a timely and safe manner

Learning Outcomes

Details of Learning Activities

Students will gain hands-on experience with laboratory equipment, reagents and real blood samples (from ARCBS donors) in a simulated working laboratory.

Routine tests include quality assurance procedures, ABO and Rh(D) blood grouping, screening for unexpected antibodies, Direct Antibody Test, and neonatal / maternal investigations

Features of fresh and processed blood products, and selection criteria for safe transfusion will be identified.

A visit to the Australian Red Cross Blood Service Melbourne Processing Centre will link the laboratory and theory material to workplace practice.

In-class exercises at the bench, on paper, kinaesthetic learning and group work will reinforce the theory.

Teaching Schedule

 Week Date    Theory Topic Practical Laboratory Skill  Assessment 
 1  8/7  Introduction to transfusion
 Managing information safely
 Equipment & reagents  
 2  15/7  What's a blood group? 
 Are you positive?
 ABO Blood groups on tiles  
 3  22/7  Antibodies meet antigens:
 Transfusion reactions
 Request forms
 Pretransfusion laboratory guidelines
 4  29/7  What else is on a red cell membrane?
 Other blood group systems
 Pretransfusion sample validity
 ABO group (forward & reverse) 2 patients
 5  5/8  Laboratory testing techniques: 
 CAT and automation
 Tube ABO group (forward & reverse): 2 patients  Assessment 1: Pretransfusion sample validity
 in class
 6  12/8  What's missing?
 Transfusing blood products
 Test yourself: blood groups
 Choosing compatible rbc units
 Formative assessment:
 Blood Groups
 7  19/8  Avoiding disasters; maintaining quality  CAT Blood group: 2 patients; choose rbc units
 NATA 'spot audit' of workbooks
 8  26/8  Exam Week    Assessment 2: Exam- dry case studies
   2/9  Mid Semester break    
9  9/9  Antibody Screen  CAT Group & Antibody screen (G&S): 2 patients  
10  16/9  When the antibody screen is positive
 Bench crossmatch
 Investigating a positive antibody screen
 CAT G&S plus XM: 2 patients, DAT
11  23/9  Haemolytic transfusion reactions  Tube antibody screen
 Follow-up investigation; antibody panels
12  30/9  ARBCS Excursion tbc   Assessment 3: Blood Products
 online 30 Sep
13  7/10  Maternal antenatal screening, HDNB
 Transfusing neonates 
 'Mother & baby' investigation:
 G&S / DAT
14  14/10  Revision  Practice run: practical assessment  
15  21/10  PRACTICAL ASSESSMENT   Assessment 5: Prac Test
16  28/10  Exam week   Assessment 4: Audit of work portfolio
 Assessment 6: Final exam




Learning Resources

Prescribed Texts


Other Resources

Up-to-date links to Transfusion websites and other relevant resources are available on Blackboard / LMS and within course material.

Overview of Assessment

written tests

in-class exercises

industry visit report

poster presentation

Portfolio of laboratory logbook & worksheets

Assessment Tasks


Assessment 1: Pretransfusion sample validity 20%
Week 5 In class

Assessment 2: Case Studies 30%
Week 8

Assessment 3: Blood products worksheet 15%
 submit online Sept 30th 

Assessment 4: Work portfolio Audit 15%
Week 16

Assessment 5   S/NYS
Practical assessment – in class week 15 

Assessment 6: Final exam 20%
Week 16

Assessment Matrix

Other Information

Assessment information

This course is graded in accordance with competency-based assessment, but which also utilise graded assessment

CHD Competent with High Distinction (80 – 100%)
CDI Competent with Distinction (70 – 79%)
CC Competent with Credit (60 – 69%)
CAG Competency Achieved – Graded (50 – 59%)
NYC Not Yet Competent
DNS Did Not Submit for assessment


  • To pass the course you need to pass, on average, each type of assessment (exams, pracs, and assignments etc.) For example, if there are two exams you need to have an average of 50% to pass and you also need to have passed the other assessment types. You can’t make up marks from one type of assessment to another (e.g. pass the exams but fail the prac component)
  • A minimum of 80% attendance at practical classes is required to pass this course. If you are sick you should submit a medical certificate to 6th floor office addressed to the Program Coordinator. If you miss a practical class that is a prac report write up you need to provide a medical certificate (or other evidence) and arrange an alternative report with your teacher
  • Late work that is submitted without an application for an extension (see below) will not be corrected.
  • APPLICATION FOR EXTENSION OF TIME FOR SUBMISSION OF ASSESSABLE WORK - A student may apply for an extension of up to 7 days from the original due date. They must lodge the application form (available online at least 24 hours before the due date. The application should be emailed to the Program Coordinator ( Students requiring longer extensions must apply for SPECIAL CONSIDERATION.
  • For missed assessments such as exams- you (& your doctor if you are sick) must fill out a special consideration form. This form must be lodged online with supporting evidence prior to, or within, 5 days of the scheduled time of examination


Plagiarism is the presentation of the work, idea or creation of another person as though it is your own. It is a form of cheating and is a very serious academic offence that may lead to expulsion from the University. Plagiarised material can be drawn from, and presented in, written, graphic and visual form, including electronic data and oral presentation. Plagiarism occurs when the origin of the material used is not appropriately cited. It also occurs through enabling plagiarism, which is the act of assisting or allowing another person to plagiarise or to copy your own work. Please make sure you consider this carefully in completing all your work and assessments in this course and if you are unsure about whether you might have plagiarised, seek help from your teacher. 


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