Course Title: Perform biological procedures
Part B: Course Detail
Teaching Period: Term1 2018
Course Code: BIOL5231C
Course Title: Perform biological procedures
School: 174T School of VE Engineering, Health & Science
Campus: City Campus
Program: C5362 - Diploma of Laboratory Technology (Pathology Testing)
Course Contact: Namrita Kaul
Course Contact Phone: +61 3 9925 4837
Course Contact Email: namrita.kaul@rmit.edu.au
Name and Contact Details of All Other Relevant Staff
Teacher: Penny Trseise
penelope.tresise@rmit.edu.au
Nominal Hours: 130
Regardless of the mode of delivery, represent a guide to the relative teaching time and student effort required to successfully achieve a particular competency/module. This may include not only scheduled classes or workplace visits but also the amount of effort required to undertake, evaluate and complete all assessment requirements, including any non-classroom activities.
Pre-requisites and Co-requisites
This unit of competency has no prerequisites.
Assumed knowledge: BIOL5230C Perform chemical tests and procedures
Course Description
This unit of competency covers the ability to interpret work requirements, prepare samples, conduct pre-use and calibration checks on equipment, and perform routine biological procedures. These procedures may involve several steps and are used to classify cell types, species and biologically active compounds by analysing their biological and chemical characteristics. This unit includes data processing, interpretation of results and troubleshooting obvious departures from standard procedures.
National Codes, Titles, Elements and Performance Criteria
National Element Code & Title: |
MSL974006 Perform biological procedures |
Element: |
1. Interpret and schedule work requirements |
Performance Criteria: |
1.1 Review work request to identify samples, required procedures and materials/equipment/instruments involved |
Element: |
2. Receive and prepare biological samples |
Performance Criteria: |
2.1 Log samples using standard operating procedures (SOPs) |
Element: |
3. Perform techniques that assist in the classification of a cell or species |
Performance Criteria: |
3.1 Select suitable techniques in accordance with workplace requirements and methods |
Element: |
4. Perform techniques that analyse biological activity |
Performance Criteria: |
4.1 Select suitable techniques in accordance with workplace requirements and methods |
Element: |
5. Process and interpret data |
Performance Criteria: |
5.1 Record test data noting atypical observations 5.2 Construct calibration graphs and compute results for all samples from these graphs |
Element: |
6. Maintain a safe work environment |
Performance Criteria: |
6.1 Use established safe work practices and personal protective equipment (PPE) to ensure personal safety and that of other laboratory personnel |
Element: |
7. Maintain laboratory records |
Performance Criteria: |
7.1 Record approved data into workplace system 7.2 Maintain confidentiality and security of workplace information and laboratory data |
Learning Outcomes
Details of Learning Activities
• Teacher directed delivery of lessons
• Class discussion
• Case study analysis
• Laboratory experiments
• Record keeping of experiments
• Research activities
• Site visit
Teaching Schedule
Semester 1: Cell Biology
Date |
Topic/Prac |
Class structure |
Assessment/Due date |
1. 05/02 |
Topic 1 - A view of life
|
Intro to course and assessment items. Blackboard tour and room induction Topic 1 lesson |
|
2. 12/02 |
Topic 2: Cell structure and function 1
|
Review Topic 1 Topic 2 lesson
|
|
3. 19/02 |
Topic 3 : Cell structure and function 2
|
Review of Topic 2 Topic 3 lesson Prac 1 preparation
|
|
4. 26/02 |
Prac 1: Classification of cell types
|
Review of Topic 3 Practical 1 |
Assessment 1: Classification of cell types (Group prac report) 7.5 % DUE Week 7 Sunday |
5. 5/03 |
Tutorial: Scientific report writing, Turnitin and referencing tutorial
|
Bring Assessment 1 results and intro to class for feedback and assistance |
|
6. 12/03 |
Topic 4: Cell membrane and cell transport
|
Topic 4 lesson Prac 2 preparation
|
|
7. 19/03 |
Prac 2: Diffusion across a semipermeable membrane
|
Review of Topic 4 Prac 2 |
|
8. 26/03 |
Topic 5 - How cells obtain energy
|
Topic 5 lesson Electron transport chain modelling Cast study
|
|
06/04 |
No class - Student vacation
|
|
|
9. 13/04 |
Assessment week - No test - No Class
|
|
|
10b. 20/04 |
Topic 6: Photosynthesis
|
Review of Topic 5 Topic 6 lesson Design experimental variables for Prac 3 |
|
11. 27/04 |
Practical 3: Photosynthesis in leaf disks
|
Review of Topic 6 Prac 3 |
Assessment 2: Photosynthesis practical report (Group prac report) 7.5 % DUE - Week 13 Sunday |
12. 04/05 |
Topic 7: Cell reproduction & Prac 4 - : Mitosis under the microscope
|
Topic 7 lesson Prac 4
|
|
13. 11/05 |
Topic 8: The immune system 1
Prac 5: Inflammation and phagocytosis
|
Review of Topic 7 Topic 8 lesson - Normal response to trauma and infection & anatomy of the immune system Prac 5
|
|
14. 18/95 |
Topic 9: The immune system 2
|
Review of Topic 8 Topic 9 lesson - Killing invaders, cell mediated immunity & humoral immunity Case study analysis and class discussion
|
|
15. 25/05 |
Revision |
Practice test discussion |
|
16. 31/05 |
Assessment week |
|
Assessment 3: Test 1 Topic 1 – 9 (15%)
|
Semester 2: Biochemistry
Week & Date |
Topic |
Practical (Tuesday) |
Assessment |
1 Week beginning Mon 3rd July |
Introduction to Biomolecules Structure and function: Carbohydrates |
PRAC 1a Preparing solutions-Biuret & Saturated SAS PRAC 1b Risk assessments |
|
2 Mon 10th July |
Structure and function: Proteins
|
PRAC 2 Carbohydrates
|
Assessment 4 - Practical report Carbohydrates (Due week 4) 10% |
3 Mon 17th July |
Structure and function: Enzymes |
PRAC 3 Spectrophotometric Protein Determination Biuret Method (5 point curve)
|
|
4 Mon 24th July |
Structure and function: Lipids
|
PRAC 4 Spectrophotometric Protein Determination Biuret Method (1 point curve and Calculation) (1 of 5)
|
|
5 Mon 31st July |
Structure and function: Nucleic acids |
PRAC 5 Enzymes &
|
|
6 Mon 7th August |
DNA replication
|
PRAC 6 Mini Prep (Kit) & |
|
7 Mon 14th August |
Revision |
PRAC 4 Rapid Spectrophotometric Protein Determination Biuret Method (Calculation only) (4 of 5) & Peer review of log books |
|
8 Mon 21st Aug
|
Exam week |
|
Assessment 5: Test 2 (25%) |
Mon 28th Aug
|
Student vacation |
- |
|
9 Mon 4th Sep |
The Biochem laboratory and techniques 1 Tools and techniques
Complex liquids: Milk Composition of blood |
PRAC 7 Fractionation of Milk & |
Assessment 6: Practical report Rapid Biuret Protein Determination 10% DUE - Week 11
|
10 Mon 11th Sep |
The Biochem laboratory and techniques 2 Sample prep and quality control |
Practical Test: Rapid Spectrophotometric Protein Determination Biuret Method (Calculation only)
|
Assessment 7: Practical Test: Rapid Biuret Protein (S/NYS) |
11 Mon 18th Sep |
Transcription |
PRAC 8 Electrophoresis of Dyes
|
|
12 Mon 25th Sep |
Translation |
PRAC 9 DNA: Restriction enzyme digest and electrophoresis (using plasmid from mini prep)
OR
PRAC 10 Immunoblot
|
|
13 Mon 2nd Oct
|
Clinical Biochemistry 1 |
PRAC 9 DNA: Restriction enzyme digest and electrophoresis (using plasmid from mini prep)
OR
PRAC 10 Dot blot
|
|
14 Mon 9th Oct
|
Clinical Biochemistry 2 |
Log book grading (face to face)
|
Assessment 8: |
15 Mon 16th Oct |
Revision |
Supplementary practical test
|
|
16 Mon 24th Oct |
Exam Week |
|
Assessment 9: Test 3 (25%)
|
Learning Resources
Prescribed Texts
Introduction to General, Organic & Biochemistry (10th Edition) Bettelheim, Brown & March |
References
Other Resources
Overview of Assessment
Written laboratory reports
Site visit report
Written tests
Laboratory skills assessment
Laboratory logbook
Assessment Tasks
Semester 1
Assessment 1: Group practial report: Classification of cell types prac (7.5 %) Week 4
Assessment 2: Practical report: Diffusion of glucose/starch across a membrane (7.5%) Week 11
Assessment 3: Test 1 Topic 1 – 9 (15%) Week 16
Semester 2
Assessment 4: Practical report Carbohydrates (10%) Due week 4
Assessment 5: Test 2 (25%) Week 8
Assessment 6: Practical report Rapid Biuret Protein Determination 10% Due week 11
Assessment 7: Practical Test: Rapid Biuret Protein (S/NYS) Week 11
Assessment 8: Log Book (S/NYS)
Assessment 9: Test 3 (25%) Week 16
*Detailed outlines and instructions on Canvas
Assessment Matrix
Other Information
Assessment Information
• This course is graded in accordance with competency-based assessment, but which also utilise graded assessment
CHD Competent with High Distinction (80 – 100%)
CDI Competent with Distinction (60 – 79%)
CC Competent with Credit (50 – 59%)
CAG Competency Achieved – Graded (0 – 49%)
NYC Not Yet Competent
DNS Did Not Submit for assessment
• To pass the course you need to pass, on average, each type of assessment (exams, pracs, and assignments etc.) For example, if there are two exams you need to have an average of 50% to pass and you also need to have passed the other assessment types. You can’t make up marks from one type of assessment to another (e.g. pass the exams but fail the prac component).
- A minimum of 80% attendance at practical classes is required to pass this course. If you are sick you should submit a medical certificate to 6th floor office addressed to the Program Coordinator. If you miss a practical class that is a prac report write up you need to provide a medical certificate (or other evidence) and arrange an alternative report with your teacher
• Late work that is submitted without an application for an extension (see below) will not be corrected.
• APPLICATION FOR EXTENSION OF TIME FOR SUBMISSION OF ASSESSABLE WORK - A student may apply for an extension of up to 7 days from the original due date. They must lodge the application form (available online http://www1.rmit.edu.au/students/assessment/extension) at least 24 hours before the due date. The application form should be emailed to the Program Coordinator ( namrita.kaul@rmit.edu.au). Students requiring longer extensions must apply for SPECIAL CONSIDERATION.
• For missed assessments such as exams- you (& your doctor if you are sick) must fill out a special consideration form. This form must be lodged online with supporting evidence prior to, or within, 48 hours of the scheduled time of examination http://www1.rmit.edu.au/students/specialconsideration
Plagiarism is the presentation of the work, idea or creation of another person as though it is your own. It is a form of cheating and is a very serious academic offence that may lead to expulsion from the University. Plagiarised material can be drawn from, and presented in, written, graphic and visual form, including electronic data and oral presentation. Plagiarism occurs when the origin of the material used is not appropriately cited. It also occurs through enabling plagiarism, which is the act of assisting or allowing another person to plagiarise or to copy your own work. Please make sure you consider this carefully in completing all your work and assessments in this course and if you are unsure about whether you might have plagiarised, seek help from your teacher.
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