Course Title: Apply routine chromatographic techniques
Part B: Course Detail
Teaching Period: Term2 2020
Course Code: BIOL5256C
Course Title: Apply routine chromatographic techniques
School: 174T School of VE Engineering, Health & Science
Campus: City Campus
Program: C5388 - Diploma of Laboratory Technology
Course Contact: Kirsten Balding
Course Contact Phone: +61 3 9925 4283
Course Contact Email: kirsten.balding@rmit.edu.au
Name and Contact Details of All Other Relevant Staff
Kirsten Balding
kirsten.balding@rmit.edu.au
Nominal Hours: 120
Regardless of the mode of delivery, represent a guide to the relative teaching time and student effort required to successfully achieve a particular competency/module. This may include not only scheduled classes or workplace visits but also the amount of effort required to undertake, evaluate and complete all assessment requirements, including any non-classroom activities.
Pre-requisites and Co-requisites
MSL974019 Perform chemical tests and procedures
Course Description
This unit of competency describes the skills and knowledge to analyse samples using routine chromatographic techniques. The unit also includes establishing client needs for routine and non-routine samples, optimising workplace procedures and instruments for specific samples, obtaining valid and reliable data and reporting test results. Personnel are required to recognise atypical test data/results and troubleshoot common analytical procedure and equipment problems.
National Codes, Titles, Elements and Performance Criteria
National Element Code & Title: |
MSL975040 Apply routine chromatographic techniques |
Element: |
1. Establish client needs and schedule analysis |
Performance Criteria: |
1.1 Record sample description, compare with specification and record and report any discrepancies 1.2 Identify non-routine samples and the possible need to vary workplace procedures 1.3 Seek advice from supervisor about any proposed variations and document all approved changes 1.4 Schedule analysis using workplace procedures |
Element: |
2. Prepare samples and standards |
Performance Criteria: |
2.1 Obtain a representative analytical portion of the laboratory sample 2.2 Prepare sample in accordance with testing requirements 2.3 Prepare validation checks for analytical portion |
Element: |
3.Set up and optimise instrument |
Performance Criteria: |
3.1 Perform pre-use and safety checks in accordance with workplace procedures 3.2 Start up and condition the instrument using workplace procedures 3.3 Optimise instrumental parameters to suit sample and test requirements 3.4 Check calibration status of instrument and perform calibration using specified standards and procedures, as required 3.5 Clean, care for and store equipment and consumables in accordance with workplace procedures |
Element: |
4. Perform analysis |
Performance Criteria: |
4.1 Measure analyte response for standards, validation checks and samples 4.2 Conduct sufficient measurements to obtain reliable data 4.3 Return instruments to standby or shutdown condition, as required |
Element: |
5.Process and analyse data |
Performance Criteria: |
5.1 Confirm data is the result of valid measurements 5.2 Perform required calculations and ensure results are consistent with standards or estimations and expectations 5.3 Record results with the appropriate accuracy, precision and units 5.4 Analyse trends in data and/or results and report out-of-specification or atypical results promptly to appropriate personnel 5.5 Troubleshoot analytical procedure or equipment problems which have led to atypical data or results |
Element: |
6. Maintain laboratory records |
Performance Criteria: |
6.1 Count cells in samples 6.2 Prepare serial dilutions of samples aseptically for culture and colony counting 6.3 Indicate the appropriate dilution necessary to reliably count organisms in a serially diluted sample 6.4 Count colonies for calculating number of viable organisms per unit volume |
Learning Outcomes
Details of Learning Activities
practical classes
Online theory
group work
worksheets
online activities
Teaching Schedule
2020
Note that the program has been altered due to COVID19 restrictions.
| Week | Date | 2020 Program | Assessment |
| 1 | 6-Jul | Paper Chromatography Theory Lab 1: Online 1.Course Overview 2.Safety in the Lab 3. Making solutions (calcs), start Paper Chromatography of food dyes analysis |
AT5: Lab Records |
| 2 | 13-Jul | Lab 2: Online Paper Chromatography of food dyes Report - in class |
AT5: Lab Records AT1: Paper chromatography report |
| 3 | 20-Jul | TLC Theory Lab 3: Online Thin Layer Chromatography of Vegetable pigments |
AT5: Lab Records AT2: Quiz |
| 4 | 27-Jul | Lab 4: Online Thin Layer Chromatography Tablets |
AT5: Lab Records |
| 5 | 3-Aug | Complete Logbook Assessments | AT5: Lab Records |
| 6 | 10-Aug | Column Chromatography Theory: Size Exclusion (SEC) Ion Exchange (IEC); Affinity chromatography (AC) |
AT3: KE questions (Test 1) AT2: Quiz |
| 7 | 17-Aug | Finalise logbook & KE questions | AT3: KE questions (Test 1) |
| 8 | 24-Aug | "HPLC theory & calculations - peak area, retention time, Using standards to find the concentration of a substance" Lab 7: Online HPLC of caffeine in drinks Case studies – Common problems and Troubleshooting |
AT4: HPLC Report/ AT6: KE Questions Available (Test 2) |
| 31-Aug | Mid- semester break | ||
| 9 | 7-Sep | Theory: HPLC - isocratic v's gradient chromatography; Qualitative and Quantitative HPLC – Using standards to find what substances are in a sample and at what concentration Lab 8: HPLC of Tablets; |
AT6: KE Questions (Test 2) AT2: Quiz |
| 10 | 14-Sep | Finalise KE questions (HPLC) | AT6: Finalise KE questions (Test 2) |
| 11 | 21-Sep | LABSKILLS 1: paper chroma 1 (single conc, do individually) (SOP adjustments: non-routine samples, Sample prep: dilution) (sample 1 - dye) |
AT5: Labskills |
| 12 | 28-Sep | LABSKILLS 1: paper chroma 1 (single conc, do individually) (SOP adjustments: non-routine samples, Sample prep: dilution) (sample 2 - other dye) |
AT5: Labskills AT2: Quiz |
| 13 | 5-Oct | LABSKILLS 2: Thin Layer Chromatography of Vegetable pigments (Spinach+ red Lettuce) Needs Rf values for validity (Method optimisation & effect of the solvent) (sample prep: solvent extraction & grinding) (sample 2 vegetables) |
AT5: Labskills |
| 14 | 12-Oct | LABSKILLS 2: Thin Layer Chromatography of more Vegetable pigments (Spinach & red lettuce) Needs Rf values for validity (sample prep: solvent extraction & grinding) (sample 2 vegetables) |
AT5: Labskills |
| 15 | 19-Oct | LABSKILLS 3: TLC of Tablets - (Sample prep: grinding, filtering)(sample tablet 1) | AT5: Labskills |
| 16 | 26-Oct | LABSKILLS 3: Repeat TLC of Tablets - (Sample prep: grinding, filtering)(sample tablet 2) | AT5: Labskills/ Finalise all Labskills assessment records |
| 17 | alternative assessment (special consideration only) |
Learning Resources
Prescribed Texts
References
Other Resources
Recommended: Boyer R (2006) Biochemistry Laboratory Modern Theory and Techniques, Benjamin Cummings Call No. 572.078 B791
The text has good information about both Electrophoresis and Chromatography Techniques. There are a number of copies on the library that you can borrow.
Download your practical manual from Canvas and PRINT a copy.
Overview of Assessment
Assessment may include
observation of laboratory tasks
folio of laboratory results
online quizzes
analysis of experimental results
Assessment Tasks
AT1: Practical Report - Paper Chromatography
AT2: Knowledge Evidence Quizzes
AT3: Knowledge Evidence Test 1
AT4: Prac report- HPLC Report
AT5: Lab Records & Skills
Due dates are shown in Canvas. Weeks that each assessment is on is shown in the teaching schedule above.
AT6: Knowledge Evidence Test 2
Assessment Matrix
Other Information
This course is graded in accordance with competency-based assessment
CA Competency Achieved
NYC Not Yet Competent
DNS Did Not Submit for assessment
To pass the course you need achieve a satisfactory result for all assessments. Students may be given additional opportunities to demonstrate competence.
Late work that is submitted without an application for an extension (see below) will not be corrected.
APPLICATION FOR EXTENSION OF TIME FOR SUBMISSION OF ASSESSABLE WORK - A student may apply for an extension of up to 7 days from the original due date. They must lodge the application form (http://www1.rmit.edu.au/students/assessment/extension) at least 24 hours before the due date. The application should be emailed to the Program Coordinator. Students requiring longer extensions must apply for SPECIAL CONSIDERATION.
• For missed assessments such as tests- you (& your doctor if you are sick) must fill out a special consideration form. This form must be lodged online with supporting evidence prior to, or within, 5 days of the scheduled time of the assessment http://www1.rmit.edu.au/students/specialconsideration
Plagiarism is the presentation of the work, idea or creation of another person as though it is your own. It is a form of cheating and is a very serious academic offence that may lead to expulsion from the University. Plagiarised material can be drawn from, and presented in, written, graphic and visual form, including electronic data and oral presentation. Plagiarism occurs when the origin of the material used is not appropriately cited. It also occurs through enabling plagiarism, which is the act of assisting or allowing another person to plagiarise or to copy your own work. Please make sure you consider this carefully in completing all your work and assessments in this course and if you are unsure about whether you might have plagiarised, seek help from your teacher.
Course Overview: Access Course Overview