Course Title: Cell Biology and Tissue Culture

Part A: Course Overview

Course Title: Cell Biology and Tissue Culture

Credit Points: 12.00

Terms

Course Code	Campus	Career	School	Learning Mode	Teaching Period(s)
ONPS2427	City Campus	Undergraduate	155T Vocational Health and Sciences	Face-to-Face	Sem 1 2012, Sem 1 2014, Sem 1 2015
ONPS2427	City Campus	Undergraduate	174T School of VE Engineering, Health & Science	Face-to-Face	Sem 1 2018, Sem 1 2021
ONPS2427	City Campus	Undergraduate	535T Social Care and Health	Face-to-Face	Sem 1 2022, Sem 1 2023, Sem 1 2024, Sem 1 2025

Course Coordinator: Tino D'Angelo

Course Coordinator Phone: +61 3 9925 4380

Course Coordinator Email: tino.dangelo@rmit.edu.au

Course Coordinator Location: 51.08.02

Course Coordinator Availability: by appointment

Pre-requisite Courses and Assumed Knowledge and Capabilities

Assumed knowledge: BIOL2365 Biological principles or BIOL2564 Foundations of Human Biosciences and ONPS2429 Introduction to Genetics & Microbiology

Course Description

In this course, you will learn the practical skills of plant and animal tissue culture- the growing of cells in the laboratory. You will gain skills in aseptic technique and the use of biohazard cabinets. You will also learn about the application of tissue culture in a range of industries including research and commercial enterprises.

Objectives/Learning Outcomes/Capability Development

This course contributes to the following Program Learning Outcomes for AD012P24 Associate Degree in Applied Science:

1. Knowledge Capability
Apply broad theoretical and technical knowledge of scientific theories, principles, concepts and practice in a wide range of situations, including new situations within the professional discipline.
2. Technical Capability
Apply technical skills and tools in range of scientific activities in a safe and responsible manner to achieve the desired outcome.
3. Problem Solving
Apply critical evaluation and problem-solving skills to address real-world scientific challenges and inform evidence based decision making in paraprofessional practice.
4. Communication Capability
Communicate, report and reflect on scientific findings, to diverse audiences utilising a variety of formats employing integrity and culturally safe practices

On completion of this course you should be able to:
1. Subculture cell lines and count cells manually and using an automated system
2. Work safely and aseptically using a biohazard cabinet and understand the requirements or working in a PC2 laboratory
3. Freeze, store and recover cells and prepare tissue culture media and reagents
4. Identify and describe culture and cell morphology using the inverted microscope
5. Maintain cell culture records and logs
6. Identify and diagnose types and sources of contamination and carry out the remediation actions required.

Overview of Learning Activities

• Face to face teaching to obtain an understanding of the theoretical fundamentals of cell biology & tissue culture
• Laboratory work to give to you the skills in a range of instrument handling and routine cellular laboratory techniques. It is also designed to build skills in critical analysis where you will be interpreting results based on your knowledge and technical capabilities.
• Simulation, videos, and laboratory sessions: to understand the key concepts of tissue culture and its practical applications, and how to apply theory to practice. The lab work/simulations allow you to develop skills in understanding principles and critical analysis.
• Working in groups to develop teamwork skills and to create a supported learning environment
• Use of Blackboard management system to access learning resources

Overview of Learning Resources

You will use the current laboratory manual for each practical class. You will need to access the student learning management system on a weekly basis to prepare for theory and practical classes. You will also need to read sections of the recommended textbook and may be referred to other resources available in the Library, such as videos and the subject learning guide.
http://rmit.libguides.com/appliedscience

Overview of Assessment

You will be assessed on how well you meet the course’s learning outcomes and on your development against the program learning outcomes. Assessment for this course is ongoing throughout the semester and feedback will be given on all assessment tasks.

Assessment 1  E-logbook 35%
Individual
This assessment supports CLO1, CLO2, CLO3, CLO4, CLO5, CLO6

Assessment 2 Research Assignment 25%
Individual
This assessment supports CLO4 

Assessment 3 Quiz 40%
Individual
This assessment supports CLO1, CLO2, CLO3, CLO4, CLO6 

Assessment 4  Practical assessment (ungraded observation)
Individual
This assessment supports CLO1, CLO2, CLO3, CLO4, CLO5, CLO6