Course Title: Cell Biology and Tissue Culture
Part A: Course Overview
Course Title: Cell Biology and Tissue Culture
Credit Points: 12.00
155T Vocational Health and Sciences
|Sem 1 2012,
Sem 1 2014,
Sem 1 2015
Course Coordinator: Judi McCann
Course Coordinator Phone: +61 3 9925 8366
Course Coordinator Email: firstname.lastname@example.org@rmit.edu.au
Course Coordinator Availability: by appointment
Pre-requisite Courses and Assumed Knowledge and Capabilities
Enforced requisites: BIOL2365 Biological principles and ONPS2429 Introduction to Genetics & Microbiology
Students are not permitted to enrol in this course until the prerequisite is satisfied by a pass grade in the above specified RMIT courses, or credit granted for the prerequisite course.
In this course, you will learn the practical skills of plant and animal tissue culture- the growing of cells in the laboratory. You will gain skills in aseptic technique and the use of biohazard cabinets. You will also learn about the application of tissue culture in a range of industries including research and commercial enterprises.
Objectives/Learning Outcomes/Capability Development
This course contributes to the following Program Learning Outcomes for AD012 Associate Degree in Applied Science:
1. Knowledge Capability
This is the understanding of appropriate and relevant fundamental and applied scientific knowledge with the ability to use and apply that knowledge in a wide range of situations, including new situations within the professional discipline.
2. Technical Capability
This is the awareness, selection and use of appropriate laboratory and other materials and equipment and the ability to employ them in a safe and responsible manner to achieve the desired outcome.
3. Problem Solving
The ability to apply scientific principles and methods to identify and solve problems associated with a particular area of professional expertise.
4. Communication Capability
This capability is being able to communicate by means of oral, written, symbolic and pictorial presentations to a wide range of audience types as well as an understanding of t.he systems of communication in science.
Laboratory work, by its very nature, involves the ability to work well as part of a team. You will learn to contribute in a constructive manner to group and team activities and decision making processes.
On completion of this course you should be able to:
1. Subculture cell lines and count cells manually and using an automated system
2. Work safely and aseptically using a biohazard cabinet and understand the requirements or working in a PC2 laboratory
3. Freeze, store and recover cells and prepare tissue culture media and reagents
4. Identify and describe culture and cell morphology using the inverted microscope
5. Maintain cell culture records and logs
6. Identify and diagnose types and sources of contamination and carry out the remediation actions required.
Overview of Learning Activities
• Face to face teaching to obtain an understanding of the theoretical fundamentals of cell biology & tissue culture
• Laboratory work to give to you the skills in a range of instrument handling and routine cellular laboratory techniques. It is also designed to build skills in critical analysis where you will be interpreting results based on your knowledge and technical capabilities.
• Simulation, videos, and laboratory sessions: to understand the key concepts of tissue culture and its practical applications, and how to apply theory to practice. The lab work/simulations allow you to develop skills in understanding principles and critical analysis.
• Working in groups to develop teamwork skills and to create a supported learning environment
• Use of Blackboard management system to access learning resources
Overview of Learning Resources
You will use the current laboratory manual for each practical class. You will need to access Blackboard on a weekly basis to prepare for theory and practical classes. You will also need to read sections of the recommended textbook and may be referred to other resources available in the Library, such as videos and the subject learning guide.
Overview of Assessment
This course has no hurdle requirements.
Early Assessment Task: Practical report
This assessment task supports CLOs 1, 2 & 3
Assessment Task 2: written exams
This assessment task supports CLOs 2, 5 & 6
Assessment Task 3: Research assignment
This assessment task supports CLO 4
Assessment 4: Logbook
This assessment supports CLOs 4 & 5
Assessment 5: Practical test
Weighting satisfactory completion
This assessment supports CLOs 1, 2, 3 & 4