Course Title: Perform aseptic techniques

Part B: Course Detail

Teaching Period: Term1 2021

Course Code: BIOL5242C

Course Title: Perform aseptic techniques

School: 174T School of VE Engineering, Health & Science

Campus: City Campus

Program: C4397 - Certificate IV in Laboratory Techniques

Course Contact: Rauha Quazi

Course Contact Phone: +61 3 9925 4277

Course Contact Email:

Name and Contact Details of All Other Relevant Staff

Andrea McAdam

Nominal Hours: 40

Regardless of the mode of delivery, represent a guide to the relative teaching time and student effort required to successfully achieve a particular competency/module. This may include not only scheduled classes or workplace visits but also the amount of effort required to undertake, evaluate and complete all assessment requirements, including any non-classroom activities.

Pre-requisites and Co-requisites


Course Description

This unit of competency describes the skills and knowledge to perform aseptic techniques to maintain the integrity of both the sample source and the sample. It applies to sampling techniques in tissue culture and to generic microbiological procedures.
This unit of competency applies to laboratory assistants and technicians working in the field or laboratory in the biomedical, biological, food processing and environmental industry sectors. 

National Codes, Titles, Elements and Performance Criteria

National Element Code & Title:

MSL973016 Perform aseptic techniques


1. Prepare for aseptic sampling or transfer

Performance Criteria:

1.1 Check the sampling procedure conforms with the required sampling plan

1.2 Use specified personal protective equipment (PPE)

1.3 Prepare the work area for safe and effective sample transfer

1.4 Select equipment and materials specified by the procedure

1.5 Organise equipment to minimise contamination during manipulations

1.6 Label containers for clear identification

1.7 Record details in relevant log or database

1.8 Macroscopically assess media and/or reagents to confirm sterility before use


2. Transfer materials aseptically

Performance Criteria:

2.1 Protect the integrity of the sample source

2.2 Sterilise inoculating loops and/or pipette where used to prevent contamination

2.3 Perform transfer while minimising opportunities for contamination and cross-infection

2.4 Protect the integrity of the sample source and destination after transfer, and before sealing the transport or culture vessel

2.5 Re-sterilise inoculating loops, minimising the generation of aerosols as required

2.6 Perform quality control checks to confirm aseptic transfer was successful

2.7 Label transport or culture vessels for clear identification


3. Maintain work area and equipment to prevent cross-infection and contamination

Performance Criteria:

3.1 Place disposable and reusable items into relevant receptacles

3.2 Clean and disinfect work area and equipment after use

3.3 Transport disposable and reusable contaminated materials to relevant areas for disinfection, sterilisation and cleaning or disposal

3.4 Follow personal hygiene procedures

Learning Outcomes

Details of Learning Activities

Learning activities include interactive online classes, practical laboratory exercises, and individual study

Teaching Schedule



Laboratory Practical Classes
in split practical groups

Theory Topics 
Online Tues | Friday



8 Feb

in Collaborate

A1-1 Introduction to aseptic technique

A1-2 Patient specimens

AT2 Log book 
submit after each PRAC
Practice Quiz


15 Feb

PRAC 1 -1


Introduction to aseptic techniques and lab

1. Using loop
2. Prepare lawn culture
3. Aseptic transfer 


A2-1 Culture techniques

A2-2 Sterilisation & autoclaves


WORKSHEET A1 due 21/2

Log book
Practice Quiz


22 Feb

PRAC 1 -2

A2-3 Bacteria

Prepare: Streaking for single colonies


WORKSHEET A2 due 28/2

Log book


1 Mar

PRAC 2 -1


Streaking & swabbing

1. Review results of PRAC 1

2. Streak for single colonies

3. Environmental sample

 A3-1 Media

Log book
Practice Quiz


8 Mar

Public Holiday

A3-2 Disinfection

A3-3 Cross-contamination

WORKSHEET A3 due 14/3

Log book
Practice Quiz


15 Mar

PRAC 2 -2

A4-1 Reading plates

A4-2 Gram staining


22 Mar

PRAC 3 -1


More streaking plus colony morphology

1. Review results of PRAC 2

2. Streak for pure culture

3. Describe colonies on media

A5-1 Antibiotic sensitivities

A5-2 Biosafety cabinets

WORKSHEET A4 done in lab PRAC 3
submit A4 with Log book

Practice Quiz

 WORKSHEET A5 due 8/3


29 Mar

PRAC 3 -2

TUES 30 Mar

Revision exercises to prepare for Quiz assessment

FRI 9 April



[5 April]

 Mid semester break
Thurs 1 April- Wednesday 7 April

due 11 April


12 April


all groups


Online quiz in class time

AT1 Practical Assessment 

AT3 Knowledge Test


Learning Resources

Prescribed Texts


Other Resources

Overview of Assessment

Assessments will include observation of skills in the laboratory, logbook and theory test

Assessment Tasks

Assessment Task 1: Practical
AT1 Lab skills assessment

Observed performance of laboratory tasks
Date: Final week

Assessment Task 2: Portfolio
AT2 Logbook and worksheets

Record of laboratory results (practical lab and online work)
Due: Submitted weekly; Final week is last date for corrections 

Assessment Task 3: Knowledge
AT3 Theory test

Summative online quiz – three attempts
Date: Final week 

Assessment Matrix

Other Information

This course is graded in accordance with competency-based assessment

CA Competency Achieved  
NYC Not Yet Competent
DNS Did Not Submit for assessment

To pass the course you need achieve a satisfactory result for all assessments. Students may be given additional opportunities to demonstrate competence. 

Late work that is submitted without an application for an extension (see below) will not be corrected.

APPLICATION FOR EXTENSION OF TIME FOR SUBMISSION OF ASSESSABLE WORK - A student may apply for an extension of up to 7 days from the original due date. They must lodge the application form ( at least 24 hours before the due date. The application should be emailed to the program Coordinator ( Students requiring longer extensions must apply for SPECIAL CONSIDERATION (see below).

• For missed assessments such as tests- you (& your doctor if you are sick) must fill out a special consideration form. This form must be lodged online with supporting evidence prior to, or within, 5 days of the scheduled time of the assessment (

•  Plagiarism is the presentation of the work, idea or creation of another person as though it is your own. It is a form of cheating and is a very serious academic offence that may lead to expulsion from the University. Plagiarised material can be drawn from, and presented in, written, graphic and visual form, including electronic data and oral presentation. Plagiarism occurs when the origin of the material used is not appropriately cited. It also occurs through enabling plagiarism, which is the act of assisting or allowing another person to plagiarise or to copy your own work. Please make sure you consider this carefully in completing all your work and assessments in this course and if you are unsure about whether you might have plagiarised, seek help from your teacher.

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