Course Title: Perform aseptic techniques

Part B: Course Detail

Teaching Period: Term2 2009

Course Code: ONPS5197C

Course Title: Perform aseptic techniques

School: 155T Vocational Health and Sciences

Campus: City Campus

Program: C5182 - Diploma of Laboratory Technology (Pathology Testing)

Course Contact: Dina van Ruyven

Course Contact Phone: (03) 9925 4287

Course Contact Email:

Name and Contact Details of All Other Relevant Staff

Nominal Hours: 40

Regardless of the mode of delivery, represent a guide to the relative teaching time and student effort required to successfully achieve a particular competency/module. This may include not only scheduled classes or workplace visits but also the amount of effort required to undertake, evaluate and complete all assessment requirements, including any non-classroom activities.

Pre-requisites and Co-requisites

This unit of competency has no prerequisites.

Course Description

This unit of competency covers the ability to perform aseptic techniques to maintain the integrity of both the sample source and the sample. It applies to sampling techniques in tissue culture and to generic microbiological procedures.

National Codes, Titles, Elements and Performance Criteria

National Element Code & Title:

PMLTEST305B Perform aseptic techniques


Maintain work area and equipment to prevent cross-infection and contamination

Performance Criteria:

Sterilises equipment as required to prevent cross contamination of work area,personnel and environment


Prepare for aseptic sampling or transfer

Performance Criteria:

Prevents cross contamination of sample source and sample


Transfer materials aseptically

Performance Criteria:

Manipulates equipment toprevent contamination of of culture medium during transfer

Learning Outcomes

Details of Learning Activities

Classroom teaching
Laboratory sessions                                    

Teaching Schedule

Asepsis – what is it?
Bacteria intro
Biohazard cabinet theory and use.
Correct technique for transfer
Media intro
Autoclave theory27.07.09
Media theory3.8.09
Disinfection and sterility theory11.8.09
Assess technique-self assessment
Prion, fungi, viral theory
Prac assessment24.8.09
Theory test 31.08.09
Clinical bacterial culture- single colony culture theory
G stain theory Tests for bacterial ID- single colony
Clinical applications

Contamination causes and effects- food, wounds etc.14.9.09
Transport media, special media unusual organisms21.9.09
Bacteriocidal agents,antisepsis theory, cross contamination12.10.09
Review/Test19.10.09 /26.9.09

Learning Resources

Prescribed Texts


Other Resources

Overview of Assessment

Assessments for this course typically consist of:
• Class activities
• Assignments
• Presentations
• Exam

Assessment Tasks

Theory tests (2) 30% each at end of each term

Practical reports (2) 20%

Assignment on disinfection/autoclave 20%

Demonstrate competency in aseptic  transfer giving no contamination of products

Demonstrate competency in observation of OHS guidelines 

Assessment Matrix

Prepare for aseptic transferObservation in practical classes and tests

Basic bacteriology theory

Theory of disinfection, sterilisation

Transfer material aseptically

Self assessment of product of aseptic transfer

Production of sterile plates and non contaminated media during class

Theory of biohazard cabinet and autoclave use

Single bacterialcolony production theory.

Maintain work area and equipment to prevent contamination

Production of non contaminated work

OHS guidelines followed

Reasons for contamination of media and plates

Correct bacteriolgy OHS procedures theory

Course Overview: Access Course Overview