Course Title: Prepare tissue and cell cultures

Part B: Course Detail

Teaching Period: Term1 2010

Course Code: ONPS5208C

Course Title: Prepare tissue and cell cultures

School: 155T Vocational Health and Sciences

Campus: City Campus

Program: C5182 - Diploma of Laboratory Technology (Pathology Testing)

Course Contact: Geraldene Fleishman

Course Contact Phone: (03) 9925 4287

Course Contact Email:

Name and Contact Details of All Other Relevant Staff

Nominal Hours: 50

Regardless of the mode of delivery, represent a guide to the relative teaching time and student effort required to successfully achieve a particular competency/module. This may include not only scheduled classes or workplace visits but also the amount of effort required to undertake, evaluate and complete all assessment requirements, including any non-classroom activities.

Pre-requisites and Co-requisites

PMLTEST305B - Perform aseptic techniques.

Course Description

This unit of competency covers the ability to prepare primary tissue cultures for applications, such as maintenance of animal cell lines and propagation of plants by tissue culture and basic subculture procedures. Personnel are required to manipulate equipment and materials and samples to prevent contamination at all preparation stages. They will have ready access to enterprise procedures and will work under direct supervision.

National Codes, Titles, Elements and Performance Criteria

National Element Code & Title:

PMLTEST412A Prepare tissue and cell cultures


Maintain records

Performance Criteria:

5.1 Maintain records of batches of media and test data
5.2 Ensure records of tissue cultures are retrievable, legible and accurate
5.3 Ensure records conform to information management, records, quality system and legal requirements.


Monitor tissue or cell culture

Performance Criteria:

4.1 Incubate culture in specified conditions
4.2 Monitor growth of culture and record appearance and characteristics
4.3 Report presence or absence of contamination
4.4 Subculture the culture to continue the cell line
4.5 Dispose of biohazardous and other laboratory waste safely


Prepare and test cell and tissue culture media

Performance Criteria:

2.1 Select and confirm media specifications and processes/methods
2.2 Prepare culture media to suit the application
2.3 Sterilise culture media and check for sterility
2.4 Perform quality control checks to ensure that culture media is fit for purpose
2.5 Store culture media in accordance with specifications


Prepare tissue or cell cultures

Performance Criteria:

3.1 Select tissue/cell sample to optimise growth and prepare it for culture
3.2 Add specified growth agents and/or nutrients
3.3 Inoculate culture medium using aseptic techniques


Work safely according to the legal and regulatory framework

Performance Criteria:

1.1 Ensure work practices and personal actions conform to regulations, codes, guidelines and enterprise quality assurance procedures
1.2 Identify hazards and enterprise controls associated with the sample, preparation methods, reagents and equipment
1.3 Select, fit and use personal protective clothing and safety equipment
1.4 Address hazards and incidents as they arise
1.5 Maintain a chain of custody, traceable to the worker, for all cells and tissues

Learning Outcomes

Details of Learning Activities

Learning activities for this course will include:
Teacher directed delivery of lessons
Whole class Discussion
Pair/group discussion and self assessment
Laboratory experiments
Record keeping of experiments
Research activities

See the Teaching Schedule (below) for details.

Teaching Schedule

9.2Introduction to tissue culture and biotechnologyLab equipment
16.2Requirements of a tissue culture labPipette prac and online pod cast
23.2Culture environment and media IMedia preparation 1
2.3culture environment and media 2Spot test
Media preparation 2

Pipette practical
9.3Media preparation and filtrationcell subculture practical group 1
16.3sterilisation and disinfectioncell subculture practical 2
hand back of pipette practical
23.3cell growth, cell counts and dell lines 
30.3revision on line cell subculture practical due
20.4Characterization of cellsRelease and talk about essay topic
 Journal article round table
4.5 Cryopreservation
demonstration of cell freezing
11.5 large scale tissue culture procedures

18.5 Specialised cell lines
25.5 practical exam
16.6Additional assessment

Learning Resources

Prescribed Texts


Other Resources

Although you do not have to purchase the following book, it will be referred to quite often. All relevant pages will be made available to you:

Culture of Animal cells: A manual of basic technique, R Ian Freshney. Fifth edition Wiley -Liss Publications 2005

Overview of Assessment

Assessments for this course typically consist of:
• Class activities
• Assignments
• Presentations
• Exam

Assessment Tasks

Term one test worth 27.5 % of the total course
Term two test worth 27.5% of the total course
Practical test at the end of term two is worth 5% of the course
Essay due the 11.05.10 is worth 12.5% of the course
Talk on 11.05.10 is worth 12.5% of the course
Practical report one due on 02.03.10 is worth 5% of the whole course
Practical report two due on 13.04.10 is worth 10% of the course

Please not that this information is subject to change

Assessment Matrix

Other Information

Important Information

Submission of work & late work- Students are required to submit work on the due date at the School Office using the cover sheets provided. The work will be accepted through the School Office and you will receive a receipt which you should keep as proof of submission. Students must NOT submit work directly to the teacher.

Late submission of work will incur a penalty of 5% of the maximum mark per day unless Special Consideration is applied for in writing using the required form. Marks can be deducted until the minimum pass mark is reached, after which no further marks will be deducted. Work will not be accepted more than 1 week after the due date.

Special Consideration- Any student who, because of illness or other circumstances beyond their control, has been prevented from attending a test, or whose performance in a test was affected by illness or other circumstances beyond their control may apply for Special Consideration. This must be done within 48 hours of the missed assessment/ assignment by lodging the appropriate form (available at the 6th floor office) with medical certificate or other supporting documentation at the Reception Office on Level 6, Building 51. Note: It is the policy of the School of Life and Physical Sciences NOT to AUTOMATICALLY PROVIDE SUPPLEMENTARY OR DEFERRED EXAMS. You will be advised of the outcome of your application for Special Consideration. The taking of Annual Leave does NOT constitute grounds for special consideration.

Attendance Requirements- Student must attend 80% of practical classes to pass. Practical exercises will be chosen for presentation as formal reports. If they are not satisfactory, you will be given an opportunity to resubmit. If you are absent from a session nominated for a formal report and you have applied for special consideration, it is your responsibility to negotiate a replacement report with the teacher. You MUST submit the number of required reports in order to pass the subject.

Plagiarism- Plagiarism is the presentation of the work, idea or creation of another person as though it is your own. It is a form of cheating and is a very serious academic offence that may lead to expulsion from the University. Plagiarised material can be drawn from, and presented in, written, graphic and visual form, including electronic data and oral presentation. Plagiarism occurs when the origin of the material used is not appropriately cited.

It also occurs through enabling plagiarism, which is the act of assisting or allowing another person to plagiarise or to copy your own work. Please make sure you consider this carefully in completing all your work and assessments in this course and if you are unsure about whether you might have plagiarised, seek help from your teacher.

Students must follow all the OH&S rules for working in a PC2 laboratory

Course Overview: Access Course Overview