Course Title: Perform aseptic techniques

Part B: Course Detail

Teaching Period: Term1 2011

Course Code: ONPS5655C

Course Title: Perform aseptic techniques

School: 155T Vocational Health and Sciences

Campus: City Campus

Program: C5282 - Diploma of Laboratory Technology (Biotechnology)

Course Contact: Amberlee Mitton

Course Contact Phone: +61 3 9925 8053

Course Contact Email:

Name and Contact Details of All Other Relevant Staff

Teacher: Gwen MacDonald

Tel: 99254283

Nominal Hours: 40

Regardless of the mode of delivery, represent a guide to the relative teaching time and student effort required to successfully achieve a particular competency/module. This may include not only scheduled classes or workplace visits but also the amount of effort required to undertake, evaluate and complete all assessment requirements, including any non-classroom activities.

Pre-requisites and Co-requisites

there are no pre-requisites or co-requisites for this course


Course Description

This unit of competency covers the ability to perform aseptic techniques to maintain the integrity of both the sample source and the sample. It applies to sampling techniques in tissue culture and to generic microbiological procedures.


National Codes, Titles, Elements and Performance Criteria

National Element Code & Title:

MSL973004A Perform aseptic techniques


1. Prepare for aseptic sampling or transfer

Performance Criteria:


Ensure that any sampling procedure conforms with the requirements of the sampling plan

Use specified personal protective clothing and equipment

Prepare the work area for safe and effective sample transfer

Select equipment and materials specified by the procedure

Organise equipment to minimise contamination during manipulations

Label containers for clear identification

Record details in relevant log or database


2. Transfer materials aseptically

Performance Criteria:


Protect the integrity of the sample source by sterilising the sampling site and flaming the mouth of transport or culture vessel

Sterilise inoculating loops and/or pipette where used to prevent contamination

Perform transfer while minimising opportunities for contamination and cross-infection

After transfer, and before sealing the transport or culture vessel, flame the vessel mouth to maintain sterility

Re-sterilise inoculating loops, minimising the generation of aerosols

Streak plate inoculations to maximise potential for single colony growth and to avoid contamination

Label transport or culture vessels for clear identification


3. Maintain work area and equipment to prevent cross-infection and contamination

Performance Criteria:


Place disposable and reuseable items into relevant receptacles

Clean and disinfect work area and equipment after use

Transport disposable and reusable contaminated materials to relevant areas for disinfection, sterilisation and cleaning or disposal.

Learning Outcomes

Details of Learning Activities

Practical classes, tutorials, short talks,

Teaching Schedule

28.3.11Cross infection prac
4.4.11Media (theory only)
11.4.11Patient specimen requirements
autoclave theory
Plating specimens onto HBA
Gram stain
25.4.11Read plates
Media making
Autoclave demonstration and use
2.5.11Sterilisation/disinfection Disinfection theory
Plating out
9.5.11Gram stain/
Plating out
16.5.11Biohazard cab theory
Read plates
Patient swabs
23.5.11Tutorial technique/microscopy
Read plates
30.5.11Practical competency assessment
6.6.11/13.6.11Theory test
30% 2hr

Learning Resources

Prescribed Texts


Other Resources

access to internet and library required

Overview of Assessment

Assessments for this course typically consist of:
• Class activities
• Assignments
• Presentations
• Exams

Assessment Tasks

Written theory test at end of semester  on underpinning knowledge of aseptic techniques    30%

Assignment - biohazard cabinets and autoclave   30%

Work book - record of practicals done. This shows assessment grid for practical assessment     40%

Practical assessment of aseptic procedures as described in learning elements will occur in practical classes and during formal practical assessment  Competent or not yet competent grading

Assessment Matrix

Other Information

This is taught concurrently with some areas of microscopy skills

Course Overview: Access Course Overview