Course Title: Prepare tissue and cell cultures
Part B: Course Detail
Teaching Period: Term1 2012
Course Code: ONPS5661C
Course Title: Prepare tissue and cell cultures
School: 155T Vocational Health and Sciences
Campus: City Campus
Program: C5282 - Diploma of Laboratory Technology (Biotechnology)
Course Contact: Amberlee Mitton
Course Contact Phone: +61 3 9925 8053
Course Contact Email: amber.mitton@rmit.edu.au
Name and Contact Details of All Other Relevant Staff
Teacher: Suzie Politis
Nominal Hours: 50
Regardless of the mode of delivery, represent a guide to the relative teaching time and student effort required to successfully achieve a particular competency/module. This may include not only scheduled classes or workplace visits but also the amount of effort required to undertake, evaluate and complete all assessment requirements, including any non-classroom activities.
Pre-requisites and Co-requisites
ONPS5655C Perform Aseptic techniques
ONPS 5656C Microscopy
Course Description
This unit of competency covers the ability to prepare primary tissue cultures for applications, such as maintenance of animal cell lines and propagation of plants by tissue culture and basic subculture procedures. Personnel are required to manipulate equipment and materials and samples to prevent contamination at all preparation stages. They will have ready access to enterprise procedures and will work under direct supervision.
National Codes, Titles, Elements and Performance Criteria
National Element Code & Title: |
MSL974011A Prepare tissue and cell cultures |
Element: |
1. Work safely according to the legal and regulatory framework |
Performance Criteria: |
1.1 Ensure work practices and personal actions conform to regulations, codes, guidelines and enterprise quality assurance procedures |
Element: |
2.Prepare and test cell and tissue culture media |
Performance Criteria: |
2.1 Select and confirm media specifications and processes/methods |
Element: |
3. Prepare tissue or cell cultures |
Performance Criteria: |
3.1 Select tissue/cell sample to optimise growth and prepare it for culture |
Element: |
4. Monitor tissue or cell culture |
Performance Criteria: |
4.1 Incubate culture in specified conditions |
Element: |
5. Maintain records |
Performance Criteria: |
5.1 Maintain records of batches of media and test data |
Learning Outcomes
Details of Learning Activities
• Teacher directed delivery of lessons• Whole class Discussion
• Pair/group discussion and self assessment
• Worksheets
• Laboratory experiments
• Record keeping of experiments
• Research activities
Teaching Schedule
Date Theory
1 7.2.2012
Introduction to tissue culture and aseptic technique
http://www.youtube.com/watch?v=yJ_acpKglto&feature=related
2 14.2.2012 The Tissue Culture Laboratory
3 21.2.2012
Plant Tissue culture environment
4 28.2.2012
Media for plant tissue culture
www.youtube.com/watch?v=yJ_acpKglto&feature=related
5 6.3.2012
Growth regulators
• Auxins
• Cytokinins
• Gibberellins
• Abscisic acid
• Ethylene
6 13.3.2012
Plant TC techniques I
7 20.3.2012
Plant TC techniques II
8 27.3.2012 REVISION
9 3.4.2012 Mid semester exam
10 10.4.2012 STUDENT VACATION
12 17.4.2012 Transgenic crops
13 24.4.2012 Introduction to animal tissue culture
14 1.5.2012 Culture environment and Media I
15 8.5.2012 Culture environment and Media II
16 15.5.2012 Media preparation and filtration
17 22.5.2012 REVISION
18 29.5.2012 Practical exam
19 5.6.2012 End of semester exam
12.6.2012 Additional assessment
Note that schedule may be subject to change
Learning Resources
Prescribed Texts
References
Other Resources
Overview of Assessment
Asessment for this course may include tests, practical reports, assignments and presentations
Assessment Tasks
Exam ONE 20%
Exam TWO 20%
Practical exam 20%
Essay 10%
Practical Report ONE 10%
Practical Report TWO 10%
Logbook 10%
Assessment tasks will assess the student’s ability to demonstrate the following competencies:
- OH&S requirements and code of conduct in a tissue culture laboratory
- ability to prepare reagents and tissue culture media accordingly
- culturing cells aseptically
- monitoring the growth of cultures and distinguishing any normal from abnormal cells as well as contamination
- proper record maintenance
Assessment Matrix
Course Overview: Access Course Overview