Course Title: Prepare tissue and cell cultures

Part B: Course Detail

Teaching Period: Term1 2013

Course Code: ONPS5661C

Course Title: Prepare tissue and cell cultures

School: 155T Vocational Health and Sciences

Campus: City Campus

Program: C5282 - Diploma of Laboratory Technology (Biotechnology)

Course Contact: Amberlee Mitton

Course Contact Phone: +61 3 9925 8053

Course Contact Email: amber.mitton@rmit.edu.au


Name and Contact Details of All Other Relevant Staff

Teacher: Amberlee Mitton

Contact: amberlee.mitton@rmit.edu.au

Phone # 9925 8053

Office: 51.04.01

Nominal Hours: 50

Regardless of the mode of delivery, represent a guide to the relative teaching time and student effort required to successfully achieve a particular competency/module. This may include not only scheduled classes or workplace visits but also the amount of effort required to undertake, evaluate and complete all assessment requirements, including any non-classroom activities.

Pre-requisites and Co-requisites

ONPS5655C Perform Aseptic techniques

ONPS 5656C Microscopy

Course Description

This unit of competency covers the ability to prepare primary tissue cultures for applications, such as maintenance of animal cell lines and propagation of plants by tissue culture and basic subculture procedures. Personnel are required to manipulate equipment and materials and samples to prevent contamination at all preparation stages. They will have ready access to enterprise procedures and will work under direct supervision.


National Codes, Titles, Elements and Performance Criteria

National Element Code & Title:

MSL974011A Prepare tissue and cell cultures

Element:

1. Work safely according to the legal and regulatory framework

Performance Criteria:

1.1. Ensure work practices and personal actions conform to regulations, codes, guidelines and enterprise quality assurance procedures
1.2. Identify hazards and enterprise controls associated with the sample, preparation methods, reagents and equipment
1.3. Select, fit and use personal protective clothing and safety equipment
1.4. Address hazards and incidents as they arise
1.5. Maintain a chain of custody, traceable to the worker, for all cells and tissues
 

Element:

2.Prepare and test cell and tissue culture media

Performance Criteria:

2.1. Select and confirm media specifications and processes/methods
2.2. Prepare culture media to suit the application
2.3. Sterilise culture media and check for sterility
2.4. Perform quality control checks to ensure that culture media is fit for purpose
2.5. Store culture media in accordance with specifications
 

Element:

3. Prepare tissue or cell cultures

Performance Criteria:

3.1. Select tissue/cell sample to optimise growth and prepare it for culture
3.2. Add specified growth agents and/or nutrients
3.3. Inoculate culture medium using aseptic techniques
 

Element:

4. Monitor tissue or cell culture

Performance Criteria:

4.1. Incubate culture in specified conditions
4.2. Monitor growth of culture and record appearance and characteristics
4.3. Report presence or absence of contamination
4.4. Subculture the culture to continue the cell line
4.5. Dispose of biohazardous and other laboratory waste safely
 

Element:

5. Maintain records

Performance Criteria:

5.1. Maintain records of batches of media and test data
5.2. Ensure records of tissue cultures are retrievable, legible and accurate
5.3. Ensure records conform to information management, records, quality system and legal requirements
 


Learning Outcomes



Details of Learning Activities

  • Teacher directed delivery of lessons
  • Whole class discussion
  • Pair/group discussion and self assessment
  • Worksheets
  • Laboratory experiments
  • Record keeping of experiments
  • Research activities


 


Teaching Schedule

Week Week BeginningTopic/Overview Practical/Activity/PresentationAssessment1234567a7b8910111213141516
Mon 11th FebNo Class

Activity 1: Introduction to the Biohazard hood, aseptic technique and tissue culture
 

 
Mon 18th FebTopic 1: Introduction to plant tissue culture & plant physiologyPRAC 1: Seed (Lepidium sativum) propagation 
Mon 25th FebTopic 2: Plant growthPRAC 2: Media preparation
 
 
Mon 4th MarTopic 3: Plant reproductionPRAC 3: Lepidium sativum seedling transfer 
Mon 11th MarTopic 4: Tissue culture environmentPRAC 4: Surface sterilisation preparation 
Mon 18th Mar Topic 5: Media & Growth RegulatorsActivity 2 – Library research for group presentation 
Mon 25th MarStudent VacationNo Practical 
Mon 1st AprTopic 6: Micropropagation Activity 3 – Library session to plan and prepare group presentation 
Mon 8th AprExam Week  
Mon 15th AprTopic 7: ContaminationPRAC 5: Surface sterilisation and explant of African Violet leaves  Assessment 1: Prac report 1 (PRAC 5, 6 & 7)
DUE: Week 13
 
Mon 22nd AprTopic 8: Applications of plant tissue culturePRAC 6: Effect of surface sterilisation and explant of Petunia leaves
& Collect results from prac 5
 
Mon 29th Apr Topic 9: Transgenic cropsPRAC 7: Collect and compare results from prac 6 
Mon 6th May PRAC 8: Carrot callus culture 
Mon 13th May RevisionActivity 4 – Group presentations Assessment 2: Group presentation – Transgenic crops
DUE: Draft Week 10
Presentation: Week 13
Mon 20th May PRAC 9: Collect carrot callus results

 

Mon 27th May PRAC 10 – Practical exam (Explant) Assessment 3: Practical Exam – Competency based assessment (C/NYC) (Technical skills)


Assessment 4: Log Book – Competency based assessment (C/NYC) (Observational and not keeping skills)
DUE Date: Week 15
 

Mon 3rd JunEnd of Semester ExamNo PracticalAssessment 5: End of year quiz


Learning Resources

Prescribed Texts


References


Other Resources


Overview of Assessment

Asessment for this course may include tests, practical reports, assignments and presentations


Assessment Tasks

Assessment 1: Prac report 1 (PRAC 4, 5, 6 & &)

Assessment 2: Group presentation – Transgenic crops

Assessment 3: Practical Exam

Assessment 4: Log Book

Assessment 5: End of year quiz

Competency based assessment (C/NYC)

Assessment tasks will assess the student’s ability to demonstrate the following competencies:

  • OH&S requirements and code of conduct in a tissue culture laboratory
  • Ability to prepare reagents and tissue culture media accordingly
  • Culturing cells aseptically
  • Monitoring the growth of cultures and distinguishing any normal from abnormal cells as well as contamination
  • Proper record maintenance and analysis of results


Assessment Matrix

Other Information

Assessment Information

• To pass the course you need to pass, on average, each type of assessment (exam, prac reports, and assignments etc.). For example, if there are two tests you need to have an average of 50% to pass. But you also need to have passed the other assessments as well. You can’t make up marks from one type of assessment to another (eg pass the tests but fail the prac reports).

• Late work that is submitted without an application for an extension will not be corrected.

• Extension of time for submission of assessable work- A student may apply for an extension of up to 7 days from the original due date. They must lodge the application form (available on the web http://mams.rmit.edu.au/seca86tti4g4z.pdf ) at least the day before the due date. The application is lodged with the School Admin Office on Level 6, Bdg 51. Students requiring longer extensions must apply for Special Consideration (form available on the Web). For missed assessments such as exams- you (& your doctor if you are sick) must fill out a special consideration form. This form must be lodged at the HUB or online with supporting evidence (eg medical certificate), prior to, or within, 48 hours of the scheduled time of examination.

• In order to be successful in this module, you have to achieve a pass in all of the above assessment tasks. If you miss an assessment task due to unavoidable circumstances, you need to follow the procedure of special consideration and apply within the allowed time frame.

If you have any questions about this assessment process, feel free to email me on amberlee.mitton@rmit.edu.au 
 

Course Overview: Access Course Overview