Course Title: Apply routine chromatographic techniques

Part B: Course Detail

Teaching Period: Term2 2016

Course Code: ONPS5663C

Course Title: Apply routine chromatographic techniques

School: 155T Vocational Health and Sciences

Campus: City Campus

Program: C5282 - Diploma of Laboratory Technology (Biotechnology)

Course Contact: Amberlee Mitton

Course Contact Phone: +61 3 9925 8053

Course Contact Email: amber.mitton@rmit.edu.au


Name and Contact Details of All Other Relevant Staff

Kirsten Balding

kirsten.balding@rmit.edu.au

contact by email, or talk in class

Nominal Hours: 120

Regardless of the mode of delivery, represent a guide to the relative teaching time and student effort required to successfully achieve a particular competency/module. This may include not only scheduled classes or workplace visits but also the amount of effort required to undertake, evaluate and complete all assessment requirements, including any non-classroom activities.

Pre-requisites and Co-requisites

MSL974003A Perform chemical tests and procedures
MSL973007A Perform microscopic examination
MSL974006A Perform biological procedures
MSL974001A Prepare, standardise and use solutions

Course Description

This unit of competency covers the ability to analyse samples using routine chromatographic techniques.
The unit also includes establishing client needs for routine and non-routine samples, optimising enterprise procedures and instruments for specific samples, obtaining valid and reliable data and reporting test results.
Personnel are required to recognise atypical test data/results and troubleshoot common analytical procedure and equipment problems.


National Codes, Titles, Elements and Performance Criteria

National Element Code & Title:

MSL975009A Apply routine chromatographic techniques

Element:

1. Establish client needs and schedule analysis

Performance Criteria:

1.1. Liaise with client or sample provider to determine client needs and sample history 1.2. Record sample description, compare with specification and record and report discrepancies 1.3. Identify non-routine samples and the possible need to vary enterprise procedures 1.4. Seek advice from supervisor about any proposed variations and document all approved changes 1.5. Schedule analysis using enterprise procedures

Element:

2. Prepare samples and standards

Performance Criteria:

2.1. Obtain a representative analytical portion of the laboratory sample 2.2. Prepare sample in accordance with testing requirements 2.3. Prepare validation checks for analytical portion

Element:

3. Set up and optimise instrument

Performance Criteria:

3.1. Perform pre-use and safety checks in accordance with enterprise procedures 3.2. Start up and condition the instrument using enterprise procedures 3.3. Optimise instrumental parameters to suit sample and test requirements 3.4. Check calibration status of instrument and perform calibration using specified standards and procedures, if applicable

Element:

4. Perform analysis

Performance Criteria:

4.1. Measure analyte response for standards, validation checks and samples 4.2. Conduct sufficient measurements to obtain reliable data 4.3. Return instruments to standby or shutdown condition, as required

Element:

5. Process and analyse data

Performance Criteria:

5.1. Confirm data is the result of valid measurements 5.2. Perform required calculations and ensure results are consistent with standards or estimations and expectations 5.3. Record results with the appropriate accuracy, precision and units 5.4. Analyse trends in data and/or results and report out of specification or atypical results promptly to appropriate personnel 5.5. Troubleshoot analytical procedure or equipment problems which have led to atypical data or results

Element:

6. Maintain a safe work environment

Performance Criteria:

6.1. Identify risks, hazards, safety equipment and control measures associated with sample handling, preparation and analytical method 6.2. Use personal protective equipment and safety procedures specified for test method and materials to be tested 6.3. Minimise the generation of wastes and environmental impacts 6.4. Ensure the safe disposal of laboratory wastes 6.5. Clean, care for and store equipment and consumables in accordance with enterprise procedures

Element:

7. Maintain laboratory records

Performance Criteria:

7.1. Enter approved data and results into laboratory information management system (LIMS) 7.2. Maintain equipment logs in accordance with enterprise procedures 7.3. Maintain security and confidentiality of laboratory data and enterprise information 7.4. Communicate results to appropriate personnel


Learning Outcomes



Details of Learning Activities

Learning activities for this course will include:
• Teacher directed face-to face delivery of lessons
• Whole class Discussion
• Pair/group discussion
• Small group workshops
• Online quizzes & theory activities
•  Worksheets including Revision
• Laboratory experiments
• Record keeping of experiments
• Presentations
• Research activities


Teaching Schedule

 

TERM 1

Week

Date week of

Theory (online)

Lab (Monday 4 hr)
   9 a.m.- 1 p.m.

Assessment

1

4-Jul

 

Topic 1

Paper Chromatography Theory - concepts & calcs

Lab 1

  1. Course Overview
  2. Safety in the Lab
  3. Making solutions

Logbook

2

11-Jul

Topic 2

Paper Chromatography Theory

Lab 2

Paper Chromatography of food dyes- (Method optimisation & effect of the solvent)

VALID METHOD

Logbook

Prac Report 1
(part 1-experiment)

3

18-Jul

Topic 3

TLC Theory concepts & calcs

Lab 3

Interpreting results
& Complete Group Report in class

Quiz 1
(TLC, Paper chroma)

Logbook

Prac Report 1

(part 2 analysis)

4

25-Jul

Topic 4

Size Exclusion Chromatography (SEC) Theory (also called gel filtration chromatography (GFC)

Lab 4

Thin Layer Chromatography of Vegetable pigments
Needs Rf values for validity

(Method optimisation & effect of the solvent)

Logbook

5

1-Aug

Topic 5
Ion Exchange (IE) Chromatography Theory

Lab 5

1. Solution Making for HPLC

2. SEC extraction of GFP and/or BFP from E. coli cells. Edvotek kit

Quiz 2
(SEC, IEC)

Logbook

6

8-Aug

Topic 6

  • Affinity chromatography (AC)
  • Multistep chromatography – concepts

Lab 6

  • Biuret of GFP & BFP fractions to quantify samples
  • SDS-PAGE of the GFP and BFP fractions

VALID METHOD

7

15-Aug

Revision

Logbook assessment catchup

 

8

22-Aug

Exam Week

 

Exam 1

Week of


29 Aug

Student vacation

 

 

Subject to change

 

 

 

TERM 2

Week

Date week of

Theory (online)

Prac (4 hr)

Assessment

9

5-Sep

 

Topic 7 HPLC theory & calculations - peak area, retention time, internal standards

  • Solid Phase Extraction (SPE)

Lab 7

Ion Exchange Chromatography

 

Quiz 3
(AC, HIC, SPE)

Logbook

10

12-Sep

Topic 8 HPLC - isocratic v's gradient chromatography theory

 

Lab 8

HPLC of caffeine in drinks

(whole class)
VALID METHOD

Case studies – Common problems and Troubleshooting

Prac Report 2
(part 1-experiment)

Logbook

11

19-Sep

Topic 9

Caffeine in Drinks - Using standards to find the concentration of a substance

Lab 9

1. HPLC of caffeine in drinks -

interpreting results

Prac Report 2 
(part 2-analysis)

12

26-Sep

 

Topic 10

Qualitative and Quantitative HPLC – Using standards to find what substances are in a sample and at what concentration

Lab 10

1a. Prepare Tablets for HPLC

1b. HPLC of Tablets
VALID METHOD

2. TLC of aspirin, caffeine & paracetamol
VALID METHOD

Quiz 4(HPLC)

Logbook

13

3-Oct

Topic 11

HPLC Analgesics results

Topic 12

Internal standards

Lab 11

HPLC of analgesics

interpreting results

 

Logbook due

by 5pm on Thursday
of this week.
Submit to level 6 office

14

10-Oct

Topic 13

GC theory & calculations

 

Lab 12

GC @ Applied Chemistry

Gas Chromatography of Xylene
VALID METHOD

Quiz 5
(GC Exp’t)

15

17-Oct

Revision

 

16

24-Oct

Exam Week

Exam 2

Exam 2  

17

31-Oct

Alternate assessment

(Special consideration only)

 

       

* Oral practical report = presentation to class at beginning of Thursday class

Subject to change


Learning Resources

Prescribed Texts

Perform Routine Chromatographic Techniques Practical Manual (available online)

• J Crowe, T. Bradshaw, P. Monk (2006)
Chemistry for the Biosciences. The essential concepts.
pp 270-274 Oxford University Press, Oxford.

Available from the course Blackboard or directly from the RMIT Library’s eReserve collection & is available after login from the following link:
https://equella.rmit.edu.au/rmit/file/9a3cddd5-1395-e24e-c64c-0dac8806f7bc/1/31259007839827.pdf

Also available in Carlton Library as Hardcopy : Call No. 540.078 C953


References


Other Resources


Overview of Assessment

Assessment for this course may include laboratory reports, assignments, exams and practival assessment in the laboratory


Assessment Tasks

 

Assessments

Week done

%

Assessment 1.

Theory Online Quizzes

 

Week 3: TLC, Paper chroma

Week 5: SEC, IEC

Week 6: CSL Case study

Week 9: AC, HIC, SPE

Week 12: HPLC

Week 14: GC Experiment

 

Weeks

3, 5, 6, 9, 12, 14

Must have an average pass grade for all quizzes

 

20%

 

Assessment 2.

2 Theory Exams

Exam 1 Topics: Paper Chromatography, TLC, SEC, IEC, and multistep Chromatography

Exam 2 Topics: HPLC & GC only

Endof
terms

3 and 4

25%+ 25%
= 50%

Assessment 3. Laboratory practice.

  1. 2 Prac Reports

1: Paper Chromatography
– Optimisation of the solvent

2: HPLC of Caffeine in drinks spreadsheet assignment

 

Report 1:
Week 2 – lab

Week 3 – write up

Report 2:
Complete during class of weeks
10 & 11

 

 

15%+ 15%
= 30%

  1. Logbook & Lab Skills Checklist:
    Each week’s entry is to be signed off by student and teacher when it is considered ‘satisfactory** and complete. Students must write up their logbook for at least 80% of labs.
    Only labs where the student was present may be written up.

At least 3 experiments must show Reliable and Valid*** results to pass this component. Acceptance criteria and Typical ranges are given in the lab manual or in class.

 

All lab sessions attended

Submit completed logbook by Friday 5pm of week 14

Satisfactory

 

Total

100%

 

 

Assessment Information

 

** see logbook information for what is required.

 

*** Reliable and Valid results: Reliable means that the results are within the typical range (ie reasonable results - the experiment has worked as expected. Valid means that the method uses validation checks and that the standards/controls results are within the acceptance criteria used to define validity (see lab manual). The validation checks are standards and/or controls.
Suitable methods to use for this purpose are indicated with “valid method” in the class schedule.

 

**Students must attend > 80% of practical classes AND pass all assessments to pass the course**.

 

 

 

  • To pass the course you need to pass, on average, each type of assessment (exam, prac reports, and assignments etc.). For example, if there are two tests you need to have an average of 50% to pass. But you also need to have passed the other assessments as well. You can’t make up marks from one type of assessment to another (e.g. pass the tests but fail the prac reports).
  • Extension of time for submission of assessable work- A student may apply for an extension of up to 7 days from the original due date. They must lodge the application form (available on the web  http://mams.rmit.edu.au/seca86tti4g4z.pdf ) at least the day before the due date. The application is lodged with the School Admin Office on Level 6, Bdg 51. Students requiring longer extensions must apply for Special Consideration (form available on the Web). For missed assessments such as exams- you (& your doctor if you are sick) must fill out a special consideration form. This form must be lodged at the HUB or online with supporting evidence (eg medical certificate), prior to, or within, 48 hours of the scheduled time of examination.
  • Late work that is submitted without an application for an extension will not be corrected.
  • If you miss an assessment task due to unavoidable circumstances, you need to follow the procedure of special consideration and apply within the allowed time frame.

What does “all assessments must be passed” mean exactly?

It is your responsibility to understand exactly what you need to do to pass the course, but hopefully the information below will make things clear… if you need more information, please ask a teacher J

 

This means you need to pass, on average, each type of assessment listed in the table ie:

  • Average exam mark (2 exams) is a pass,
  • Average prac report mark (2 reports) is a pass,
  • Average quiz mark (of 6 quizzes) is a pass,
  • Logbook is passed, including >80% attendance.
    If you miss a prac class you need to provide a copy of your medical certificate or other evidence (court appearance, funeral notice, statutory declaration) to the teacher.

 

Practical Reports

  • If you miss a prac report class you will only be eligible to write up a different prac if you provide a medical certificate. For this to occur you need to discuss your situation with the teacher as soon as possible. Other reasons for missing a write up prac do not mean that you can write up a different report.
  • Reports will be submitted online via blackboard at the link for each report.
    Reports will be reviewed for plagiarism by Turnitin.
    Reports found to contain an inappropriate amount of copied text will be required to be resubmitted within one week of notification by email. Students should aim to have less than 20% of text detected by Turnitin. If ‘proforma documents’ are provided to help you format your report, you should remove instructional text.
  • Please read the online anti-plagiarism statement given in blackboard submission of work via Blackboard incorporates student agreement to these conditions.
  • Practical report 2 (a spreadsheet activity) is due in the analysis session.

 

Quizzes

  • Each quiz has a published due date. Quizzes are to be done before the class in the same week to help you prepare for the class. Quizzes may include questions on safety.
  • To pass the quizzes component you must get an average pass for all quizzes (ie. 50% average). The score for quizzes is calculated from the average grade of all quizzes. If you miss a quiz and have submitted a medical certificate you may do it later than the due date as long as you contact the teacher asap after your absence.

 

Students must attend at least 80% of practical classes.

  • If you miss a prac class you should provide a copy of your medical certificate or other evidence (court appearance, funeral notice, statutory declaration) to the teacher.


Assessment Matrix

Other Information

This course is graded in accordance with competency-based assessment, but which also utilise graded assessment
CHD Competent with High Distinction (80 – 100%)
CDI Competent with Distinction (70 – 79%)
CC Competent with Credit (60 – 69%)
CAG Competency Achieved – Graded (50 – 59%)
NYC Not Yet Competent
DNS Did Not Submit for assessment

• To pass the course you need to pass, on average, each type of assessment (exams, pracs and assignments etc.) For example, if there are two exams you need to have an average of 50% to pass and you also need to have passed the other assessment types. You can’t make up marks from one type of assessment to another (e.g. pass the exams but fail the prac component).


• Late work that is submitted without an application for an extension (see below) will not be corrected.


• APPLICATION FOR EXTENSION OF TIME FOR SUBMISSION OF ASSESSABLE WORK - A student may apply for an extension of up to 7 days from the original due date. They must lodge the application form (available online http://www1.rmit.edu.au/students/assessment/extension) at least 24 hours before the due date. The application is lodged with the School Admin Office on Level 6, Bdg 51. Students requiring longer extensions must apply for SPECIAL CONSIDERATION.


• For missed assessments such as exams- you (& your doctor if you are sick) must fill out a special consideration form. This form must be lodged online with supporting evidence prior to, or within, 48 hours of the scheduled time of examination http://www1.rmit.edu.au/students/specialconsideration

Course Overview: Access Course Overview