Course Title: Perform tissue and cell culture techniques

Part B: Course Detail

Teaching Period: Term2 2013

Course Code: ONPS5664C

Course Title: Perform tissue and cell culture techniques

School: 155T Vocational Health and Sciences

Campus: City Campus

Program: C5282 - Diploma of Laboratory Technology (Biotechnology)

Course Contact: Amberlee Mitton

Course Contact Phone: +61 3 9925 8053

Course Contact Email: amber.mitton@rmit.edu.au


Name and Contact Details of All Other Relevant Staff

Amberlee Mitton

amberlee.mitton@rmit.edu.au

Nominal Hours: 100

Regardless of the mode of delivery, represent a guide to the relative teaching time and student effort required to successfully achieve a particular competency/module. This may include not only scheduled classes or workplace visits but also the amount of effort required to undertake, evaluate and complete all assessment requirements, including any non-classroom activities.

Pre-requisites and Co-requisites

The following 3 competencies are all pre-requisites:

MSL974006A Perform biological procedures

MSL973007A Perform microscopic examination

MSL973004A Perform aseptic techniques

Course Description

This unit of competency covers the ability to prepare, maintain and preserve cells and cell lines for a variety of applications, such as large scale culture, production of monoclonal antibodies, production of viral vaccines and amniocentesis studies. Personnel are required to optimise equipment setup, media and growth techniques. They are required to detect and investigate contamination and take preventative and/or corrective actions under supervision.


National Codes, Titles, Elements and Performance Criteria

National Element Code & Title:

MSL975013A Perform tissue and cell culture techniques

Element:

1. Interpret and schedule production requirements

Performance Criteria:

1.1.Review client request and confirm quantity and
nature of cells, tissue or products
1.2.Select, appropriate media, materials, equipment and
methods
1.3.Plan parallel work sequences to optimise production
1.4.Maintain a chain of custody, traceable to the worker,
for all cells and tissues

Element:

2. Work safely according to the legal and regulatory framework

Performance Criteria:

2.1.Ensure work practices and personal actions conform
to regulations, codes, guidelines and enterprise
quality assurance procedures
2.2.Identify hazards and enterprise control measures
associated with the sample, preparation methods,
reagents and equipment
2.3.Select, fit and use personal protective clothing and
safety equipment
2.4.Address hazards and incidents as they arise
2.5.Ensure the safe disposal of biohazardous materials
and other laboratory wastes

Element:

3. Assemble and maintain tissue culture equipment

Performance Criteria:

3.1.Assemble, sterilise or decontaminate equipment
according to enterprise procedures
3.2.Perform pre-use and safety checks in accordance
with relevant enterprise and operating procedures
3.3.Identify faulty or unsafe components and equipment
and report to appropriate personnel
3.4.Decontaminate area and equipment after use

Element:

4. Prepare and test cell and tissue culture media

Performance Criteria:

4.1.Confirm media specifications and processes/methods
4.2.Prepare culture media to suit client request
4.3.Sterilise culture media and check for sterility
4.4.Perform quality control checks to ensure that culture
media is fit for purpose
4.5.Store culture media in accordance with
specifications

Element:

5. Obtain, monitor and maintain tissue and cell lines

Performance Criteria:

5.1.Retrieve/obtain the cell lines or tissue sample from
fresh or preserved sources and prepare a culture
5.2.Select specified culture media and add any necessary
growth agents or nutrients
5.3.Incubate cells/tissue in specified conditions
5.4.Inoculate the media with the specified amount of
sample

5.5.Monitor growth of tissue and cell lines and products
5.6.Detect contamination and troubleshoot materials,
equipment and techniques
5.7.Passage samples by subculturing to preserve or grow
the line
5.8.Harvest cells or cell products to optimise yields

Element:

6. Preserve cells and tissues

Performance Criteria:

6.1.Select the appropriate preservation method
6.2.Preserve the cell lines or tissue in accordance with
the method
6.3.Check preserved cell lines regularly to ensure
viability is maintained

Element:

7. Maintain records

Performance Criteria:

7.1.Maintain records of batches of media and test data
7.2.Maintain records of active and stored tissue and cell
lines
7.3.Ensure records are retrievable, legible and accurate
7.4.Ensure records conform to the information
management, records, quality system and legal
requirements


Learning Outcomes



Details of Learning Activities

Teacher directed delivery of lessons
• Class discussion
• Worksheets
• Laboratory experiments
• Record keeping of experiments
• Research activities
• Library sessions


Teaching Schedule

WeekPractical/ActivityTheoryAssessment
 1.Library sessionTopic 1: Intro to animal tissue culture (work area & equipment)
&
Cell Biology: Cell structure and organelles
 
Assessment 1: History of tissue culture research assignment
DUE Date: Week 5
15%
 2.PRAC1: Aseptic technique in the biohazard cabinetTopic 2: Cell Biology: Life cycle, division and deathLogbook
 3.

PRAC 2: Pipetting and transfer of fluids

PRAC 3: Preparation of tissue culture solutions and sterility testing

Topic 3: Culture environment - Media and growth requirementsLogbook
 4.PRAC 4: Morphology of INT407 cellsTopic 4: The biology of cultured cellsLogbook
 5.PRAC 5: Feeding INT407 cellsTopic 5: Cell typesLogbook
 6.PRAC 6: Passaging INT407 cells using a split ratioTopic 6: Cell growthLogbook
 7. -Revision 
 8. -Mid-Semester ExamsAssessment 2: Mid semester exam (30%)
 9.PRAC 7: Cell counting using the manual method and automatic coulterTopic 7: Cell counts and viable cellsAssessment 3:
Practical report including peer review and edit.
Peer review and edit (C/NYC)
Report 15%
DUE Date: Week 11
 10. PRAC 8: Passaging a cell line using cell densityTopic 8: Contamination & Filtration, sterilization and disinfectionLogbook
 11. PRAC 9: Effect of pH og cell growthTopic 9: Cell preservation, storage and recoveryLogbook
  Student vacationStudent vacation 
 12.

 PRAC 10: Effect of seeding cell density on cell growth

Topic 10: Tissue and cell culture in industry 1: Stem cellsLogbook
 13. PRAC 11: Effect of FBS percentage on cell growth
 
Topic 11: Tissue and cell culture in industry 2: Monoclonal antibodiesLogbook
 14.Prac 12: Practical exam -Practical exam (C/NYC)
 15. - RevisionLogbook (C/NYC)
 16. - End of Semester ExamAssessment 4: End of Semester Exam 30%


Learning Resources

Prescribed Texts


References


Other Resources


Overview of Assessment

Assessments for this course typically consist of:
• Class activities
• Assignments
• Presentations
• Exams


Assessment Tasks

  • Assessment 1: History of tissue culture research assignment 15% DUE Date: Week 5
  • Assessment 2: Mid semester exam 30%
  • Assessment 3: Practical report including peer review and edit. Peer review and edit (C/NYC) Report 15% DUE Date: Week 11
  • Assessment 4: End of Semester Exam 30%
  • Practical Exam (C/NYC)
  • Log book (C/NYC)


Assessment Matrix

Other Information

Assessment Information
• To pass the course you need to pass, on average, each type of assessment (exam, prac reports, and assignments etc.). For example, if there are two tests you need to have an average of 50% to pass. But you also need to have passed the other assessments as well. You can’t make up marks from one type of assessment to another (eg pass the tests but fail the prac reports).
• Late work that is submitted without an application for an extension will not be corrected.
• Extension of time for submission of assessable work- A student may apply for an extension of up to 7 days from the original due date. They must lodge the application form (available on the web http://mams.rmit.edu.au/seca86tti4g4z.pdf) at least the day before the due date. The application is lodged with the School Admin Office on Level 6, Bdg 51. Students requiring longer extensions must apply for Special Consideration (form available on the Web). For missed assessments such as exams- you (& your doctor if you are sick) must fill out a special consideration form. This form must be lodged at the HUB or online with supporting evidence (eg medical certificate), prior to, or within, 48 hours of the scheduled time of examination.
• In order to be successful in this module, you have to achieve a pass in all of the above assessment tasks. If you miss an assessment task due to unavoidable circumstances, you need to follow the procedure of special consideration and apply within the allowed time frame.
If you have any questions about this assessment process, feel free to email me on amberlee.mitton@rmit.edu.au  

 

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