Course Title: Perform molecular biology tests and procedures
Part B: Course Detail
Teaching Period: Term1 2012
Course Code: ONPS5665C
Course Title: Perform molecular biology tests and procedures
School: 155T Vocational Health and Sciences
Campus: City Campus
Program: C5282 - Diploma of Laboratory Technology (Biotechnology)
Course Contact: Amberlee Mitton
Course Contact Phone: +61 3 9925 8053
Course Contact Email: amber.mitton@rmit.edu.au
Name and Contact Details of All Other Relevant Staff
Nominal Hours: 150
Regardless of the mode of delivery, represent a guide to the relative teaching time and student effort required to successfully achieve a particular competency/module. This may include not only scheduled classes or workplace visits but also the amount of effort required to undertake, evaluate and complete all assessment requirements, including any non-classroom activities.
Pre-requisites and Co-requisites
MSL974006A Perform biological procedures
MSL973007A Perform microscopic examination
MSL973004A Perform aseptic techniques
Course Description
This unit of competency covers the ability to isolate, purify, verify and manipulate biomolecules and their products. This work requires close attention to working with small volumes, multiple-step procedures and prevention of contamination. Personnel are required to apply a wide range of molecular biology tests and procedures.
National Codes, Titles, Elements and Performance Criteria
National Element Code & Title: |
MSL975014A Perform molecular biology tests and procedures |
Element: |
1. Interpret and schedule test requirements |
Performance Criteria: |
1.1. Review test request to identify samples to be tested, test method and equipment/instruments involved |
Element: |
2. Receive and handle samples |
Performance Criteria: |
2.1. Log and label samples according to enterprise procedures |
Element: |
3. Prepare equipment and reagents |
Performance Criteria: |
3.1. Set up equipment/instrumentation in accordance with test method requirements and perform pre-use and safety checks |
Element: |
4. Extract, verify and manipulate biomolecules |
Performance Criteria: |
4.1. Produce/extract biomolecules from samples using appropriate isolation methods |
Element: |
5. Process data |
Performance Criteria: |
5.1. Record test data noting atypical observations |
Element: |
6. Maintain a safe work environment |
Performance Criteria: |
6.1. Use established safe work practices and personal protective equipment to ensure personal safety and that of other laboratory personnel |
Element: |
7. Report and communicate results |
Performance Criteria: |
7.1. Record approved data into enterprise system |
Learning Outcomes
Details of Learning Activities
Practical exercises, lectures, discussions, class activities, research assignments, group work and peer teaching
Teaching Schedule
Week | Prac (Monday) | Theory (Thursday) |
1 | No class DNA revision- online quiz |
Revision of cell structure DNA structure The Cell cycle, Cell division |
2 | Kiwifruit extraction | Genetics of cancer |
3 | Wheat germ extraction |
DNA replication, PCR intro |
4 | Finish wheat germ extraction, Alu PCR prac |
PCR applications, forensics |
5 | Gel for VNTR PCR | transcription |
6 | Labour Day | DNA translation |
7 | RT PCR |
Sequencing theory |
8 | Mutations | Revision |
9 | Mid Sem Exams | Mid Sem Exams |
Easter break | Lac operon Conjugation |
|
10 | pGLO | Transformation transduction |
11 | research for Media watch assignment | Presentations |
12 | Presentations | Viruses, protoplasts, biolostics Restriction enzymes |
13 | Blue/White Cloning of a DNA Fragment and Assay of ß-galactosidase |
Host cells, vectors- plasmids |
14 | Miniprep pUC8 |
Vectors cont-phage & cosmids YAC, BAC, phagemids replica plating |
15 | Digestion of extracted plasmid, gel | Ti plasmid |
16 | revision |
|
17 | Exams | |
1 | No class | Meiosis Chromosomes |
2 | Genetic Barley | Chromosomes & sex determination Chromosomal abnormalities |
3 | Genetic Barley Prac Human Genetics Karyotype |
X inactivation Mendelian genetics 1 Mono hybrid cross |
4 | Prac 2 Complete results Prac 3 Barr bodies |
Dihybrid crosses Test cross |
5 | Genetics of D.melanogaster | Co dominance & incomplete dominance Sex linked inheritance Human genetics |
6 | ’Mendelian genetics 2 including statistics & probability (Chi Square) | Pedigrees, |
7 | Case studies | revision |
8 | exams | |
9 | Intro to Mitochondrial DNA Mitochondrial DNA activity in computer lab |
Linked genes Crossing over |
10 | Population genetics. Hardy-Weinberg Principle |
Applications of the Hardy- Weinberg Transposable elements |
mid semester break | ||
11 | Genetically modified organisms | Information analysis about GMO’s |
student holiday | ||
12 | GMO PCR | DNA libraries Screening techniques |
13 | GMO PCR Human Genome project |
Micro arrays, Bioinformatics |
14 | Internet activity: Computer assisted Sequence analysis | Applications of biotech |
15 | revision | |
16 | exams |
Learning Resources
Prescribed Texts
11th Hour Introduction to Molecular Biology, Raineri, Deanna Available on EBL : Ebook Library |
|
Torora, Funke & Case, MicrobIology An Introduction 10th Edition. Pearson Benjamin Cummings |
References
Cumming R. 2010, Human Heredity: Principles & Issues 7thed. Thomson, California USA |
|
Tortora, Funke & Case Microbiology: An Introduction |
Other Resources
Suggested Genetics/ Molecular biology Websites:
• Cold Spring Harbor Dolan DNA Learning Centre
http://www.dnalc.org/
• Biotechnology Online
http://www.biotechnologyonline.gov.au/
• Howard Hughes Medical Institute Biointeractive
http://www.hhmi.org/biointeractive/
Overview of Assessment
Asessment may include the following:
Written Tests
Practical reports
Assignments
Presentations
Assessment Tasks
To pass this course you must pass all summative assessment tasks
Formative assessment:
• Online Quizzes
• Problem sheets/work sheets.
• Feedback and self-assessment in laboratory
Summative assessment:
• 2 Theory exams per semester: 50%
• 3 Prac reports: 30%
• 1 Research assignment and/or presentation: 20%
Maintenance of laboratory logbook- competent or not yet competent
Assessment Matrix
Other Information
Plagiarism is the presentation of the work, idea or creation of another person as though it is your own. It is a form of cheating and is a very serious academic offence that may lead to expulsion from the University. Plagiarised material can be drawn from, and presented in, written, graphic and visual form, including electronic data and oral presentation. Plagiarism occurs when the origin of the material used is not appropriately cited.
It also occurs through enabling plagiarism, which is the act of assisting or allowing another person to plagiarise or to copy your own work. Please make sure you consider this carefully in completing all your work and assessments in this course and if you are unsure about whether you might have plagiarised, seek help from your teacher.
Students must attend 80% of practical classes to pass. Practical exercises will be chosen for presentation as formal reports. If they are not satisfactory, you may be given an opportunity to resubmit. If you are absent from a session nominated for a formal report and you have applied for special consideration, it is your responsibility to negotiate a replacement report with the teacher. You MUST submit the number of required reports in order to pass the subject.
Students are required to submit work on the due date at the School Office using the cover sheets provided. The work will be accepted through the School Office and you will receive a receipt which you should keep as proof of submission. Students must NOT submit work directly to the teacher.
A student may apply for an extension of up to 7 days from the original due date.
They must lodge the application form (http://mams.rmit.edu.au/seca86tti4g4z.pdf) at least the day before the due date. The application is lodged with the School Admin Office on Level 6, Bdg 51
Late work that is submitted without an application for an extension will not be corrected.
Any student who, because of illness or other circumstances beyond their control, has been prevented from attending a test, or whose performance in a test was affected by illness or other circumstances beyond their control may apply for Special Consideration. This must be done within 48 hours of the missed assessment/ assignment by lodging the appropriate form (available at the 6th floor office) with medical certificate or other supporting documentation at the Reception Office on Level 6, Building 51. Note: It is the policy of the School of Life and Physical Sciences NOT to AUTOMATICALLY PROVIDE SUPPLEMENTARY OR DEFERRED EXAMS. You will be advised of the outcome of your application for Special Consideration. The taking of Annual Leave does NOT constitute grounds for special consideration.
Course Overview: Access Course Overview