Course Title: Prepare, standardise and use solutions

Part B: Course Detail

Teaching Period: Term2 2012

Course Code: ONPS5657C

Course Title: Prepare, standardise and use solutions

School: 155T Vocational Health and Sciences

Campus: City Campus

Program: C5283 - Diploma of Laboratory Technology (Pathology Testing)

Course Contact: Amberlee Mitton

Course Contact Phone: +61 3 9925 8053

Course Contact Email:

Name and Contact Details of All Other Relevant Staff

** primary contact:
Kirsten Balding
9925 4283
Office 51.4.01
contact by email is the best option

Nominal Hours: 100

Regardless of the mode of delivery, represent a guide to the relative teaching time and student effort required to successfully achieve a particular competency/module. This may include not only scheduled classes or workplace visits but also the amount of effort required to undertake, evaluate and complete all assessment requirements, including any non-classroom activities.

Pre-requisites and Co-requisites

ONPS5658C Perform Chemical Tests

Course Description

This unit of competency covers the ability to prepare, standardise and monitor the quality of solutions.


National Codes, Titles, Elements and Performance Criteria

National Element Code & Title:

MSL974001A Prepare, standardise and use solutions


1. Prepare solutions

Performance Criteria:

1.1. Select appropriate procedure for solution preparation
1.2. Select equipment, materials and solvent of specified purity
1.3. Measure appropriate quantities of reagents for solution preparation and record data.
1.4. Select and assemble specified laboratory equipment and appropriate grade of glassware
1.5. Perform specified dilutions
1.6. Prepare solutions to achieve homogeneous mix of the specified concentration
1.7. Label and store solutions to maintain identity and stability


2. Standardise and use volumetric solutions

Performance Criteria:

2.1. Assemble appropriate laboratory equipment
2.2. Perform serial dilutions as required
2.3. Standardise the solution to the required specified range and precision
2.4. Label and store solutions to maintain identity and stability
2.5. Use standard volumetric solutions to determine concentration of unknown solutions


3. Calculate and record data

Performance Criteria:

3.1. Calculate specified concentrations
3.2. Use authorised procedure if data is to be modified
3.3. Estimate and document uncertainty of measurement in accordance with enterprise procedures, if required
3.4. Record all relevant details according to laboratory procedures and report results
3.5. Report concentration with appropriate units


4. Monitor the quality of laboratory solutions

Performance Criteria:

4.1. Check solutions for visual deterioration and expiry date
4.2. Restandardise or dispose of dated or deteriorated solutions
4.3. Record details and label solutions according to laboratory procedures


5. Maintain a safe work environment

Performance Criteria:

5.1. Use established safe work practices and personal protective equipment to ensure personal safety and that of other laboratory personnel
5.2. Clean up spills using appropriate techniques to protect personnel, work area and environment
5.3. Minimise generation of waste and environmental impacts
5.4. Ensure the safe collection of laboratory and hazardous waste for subsequent disposal
5.5. Store equipment and reagents as required

Learning Outcomes

Details of Learning Activities

Online quizzes
Online theory presentations, worksheets and activities
whole class discussion
Pair/group discussion
Online quizzes
Laboratory experiments
Record keeping of experiments
Research activities

Teaching Schedule

Chem Solutions: 2012 Program

Week 1: 10-Jul
Theory:Introduction to acids & bases, pH. Measuring pH, titration
Prac: Measuring pH: Indicators
Log Book only

Week 2: 17-Jul
Theory: pH & pOH, Strong & weak acids & bases, pKa
Prac: Analysis of Vinegar by Titration
Week 2 Quiz (Topics in weeks 1&2)
Prac Assessment 1: Titration done in class
Prac Report: (done in class)

Week 3: 24-Jul
Theory: Primary Standards and Standardisation of NaOH
Prac: Pipetting workshop, Standardisation of NaOH
Prac Assessment 2: Standardisation done in class

Week 4: 31-Jul
Theory: Introduction to Buffers, Predicting pH of buffer solutions
Prac: Buffers I - TAE, 1x TAE by dilution
Week 4 Quiz, (Topics in weeks 3&4), Log Book only

Week 5: 7-Aug
Theory: Buffering capacity, pH compared to pKa, buffer concentration
Prac: Buffers II - Phosphate buffers and the H-H equation
Log Book only

Week 6: 14-Aug
(Prac only)
Prac: Making TE buffer, Making a saturated solution
Week 6 Quiz (Topics in weeks 5&6), Log Book only

Week 7: 21-Aug
Revision (online)
Prac: (if required) Assessment catch up session:
(1) Titration,
(2) Standardisation

Week 8: 27-Aug
Mid Semester exams

Week 9: 4-Sep
(Theory at home): Intermolecular bonding, Water as a solvent,
Serial dilutions - refresher
Week 10: 11-Sep
(Prac only)
Prac: Solvent Properties of Water, Serial dilutions
Prac Assessment: (3) Serial dilutions
Week 11: 18-Sep
(Theory at home): Enzymes
Week 11 Quiz (Enzymes)

Week of 24-Sep: Mid semester Break

Week 12: 2-Oct
(Prac only)
Prac: IDH Enzyme Assay
Prac report: IDH assay

Week 13: 9-Oct
(Theory at home): Oxidation & Reduction (Redox) Reactions
Week 13 Quiz (Redox)

Week 14: 16-Oct
(Prac only)
Prac: Redox: Half cells and Electrochemical Series
Log Book only:Submit logbook by 5pm on Friday of week 14

Week 15: 23-Oct
Revision (at home)
Prac: (if required) Last chance Assessment catch up session
(3) Serial dilutions

Week 16: 30-Oct

Week 17: 5-Nov
Alternative assessment (Special Consideration only)

*To be confirmed

Learning Resources

Prescribed Texts

Bettelheim FA, Brown WH, Campbell MK, Farrell SO (2010) General, Organic and Biochemistry  9th Ed. Thomson/Brooks-Cole, Australia 

Lab Manual  - available from Blackboard

Blackboard for this course. Access through MyRMIT.


Other Resources

Lab coat, protective gloves, safety glasses, appropriate footwear & a logbook are required at all practical classes.

A logbook is required for this course to record all experimental details. The logbook must be brought to class each week and signed off by the teacher.
The book needs to be a bound NON-SPIRAL exercise book.


Overview of Assessment

Assessments for this course typically consist of:
• Class activities
• Assignments
• Presentations
• Exams

Assessment Tasks

To pass the course you need to achieve:
80% attendance at classes AND
An average pass grade (50%) for all quizzes AND
An average pass grade (50%) for both prac reports AND
An average pass grade (50%) for both exams AND
Competent completion of all the prac assessments AND
Competent weekly record keeping in your logbook

Assessment Tasks

5 Quizzes:  total  20%
Weeks 2, 4, 6, 11, 13

2 Prac Reports: 15% each

2 Theory Exams : 25% each

Prac Assessments:  
"Competent" at all 3 tasks
1. Titration
2. Standardisation
3. Serial dilution

Logbook: "competent" (ungraded)

If you miss a write up prac you will only be eligible to write up a different prac if you provide a medical certificate or other documentation. For this to occur you need to discuss your situation with the teacher as soon as possible.

Assessment Matrix

Other Information

Plagiarism is the presentation of the work, idea or creation of another person as though it is your own. It is a form of cheating and is a very serious academic offence that may lead to expulsion from the University. Plagiarised material can be drawn from, and presented in, written, graphic and visual form, including electronic data and oral presentation. Plagiarism occurs when the origin of the material used is not appropriately cited.
It also occurs through enabling plagiarism, which is the act of assisting or allowing another person to plagiarise or to copy your own work. Please make sure you consider this carefully in completing all your work and assessments in this course and if you are unsure about whether you might have plagiarised, seek help from your teacher.

Students must attend 80% of practical classes to pass. Practical exercises will be chosen for presentation as formal reports.
Students are required to submit work on the due date at the School Office using the cover sheets provided. The work will be accepted through the School Office and you will receive a receipt which you should keep as proof of submission. Students must NOT submit work directly to the teacher.

A student may apply for an extension of up to 7 days from the original due date.
They must lodge the application form ( at least the day before the due date. The application is lodged with the School Admin Office on Level 6, Bdg 51
Late work that is submitted without an application for an extension will not be corrected.

Any student who, because of illness or other circumstances beyond their control, has been prevented from attending a test, or whose performance in a test was affected by illness or other circumstances beyond their control may apply for Special Consideration. This must be done within 48 hours of the missed assessment/ assignment by lodging the appropriate form (available at the 6th floor office) with medical certificate or other supporting documentation at the Reception Office on Level 6, Building 51. Note: It is the policy of the School of Life and Physical Sciences NOT to AUTOMATICALLY PROVIDE SUPPLEMENTARY OR DEFERRED EXAMS. You will be advised of the outcome of your application for Special Consideration. The taking of Annual Leave does NOT constitute grounds for special consideration.

Course Overview: Access Course Overview