Course Title: Perform haematological tests

Part B: Course Detail

Teaching Period: Term1 2015

Course Code: ONPS5666C

Course Title: Perform haematological tests

School: 155T Vocational Health and Sciences

Campus: City Campus

Program: C5283 - Diploma of Laboratory Technology (Pathology Testing)

Course Contact: Amberlee Mitton

Course Contact Phone: +61 3 9925 8053

Course Contact Email:

Name and Contact Details of All Other Relevant Staff

Gwen MacDonald     Term 1 only

Brian Long  Term 2 ,3,4   

Nominal Hours: 130

Regardless of the mode of delivery, represent a guide to the relative teaching time and student effort required to successfully achieve a particular competency/module. This may include not only scheduled classes or workplace visits but also the amount of effort required to undertake, evaluate and complete all assessment requirements, including any non-classroom activities.

Pre-requisites and Co-requisites

ONPS5659C Biological procedures

ONPS5198 Perform microscopic examination

Course Description

This unit of competency describes the ability to determine levels, function, activity and interactions of cellular and plasma components of blood using tests and procedures identified with the discipline of laboratory haematology. This course does not cover the laboratory aspects of transfusion science; these are covered in "Perform immunohaematological tests". While this unit focuses on the laboratory investigation of human physiology and pathology, it reasonably describes aspects of work performed in veterinary settings.

National Codes, Titles, Elements and Performance Criteria

National Element Code & Title:

MSL975002A Perform haematological tests


1. Process samples and associated request details

Performance Criteria:

1.1. Sort specimens according to tests requested, urgent status and volume
1.2. Return samples and request forms that do not comply with requirements to their source with
reasons for non-acceptance
1.3. Log acceptable samples and request forms, applying required document tracking mechanisms
1.4. Process samples as required by requested tests
1.5. Store samples and sample components appropriately until ready for testing


2. Perform tests

Performance Criteria:

2.1. Select authorised tests that are indicated for the requested investigations
2.2. Conduct individual tests according to documented methodologies, applying required quality
control procedures
2.3. Record all results, noting any phenomena that may be relevant to the interpretation of results
2.4. Seek advice of section head or other responsible colleague when result interpretation is outside
parameters of authorised approval
2.5. Store unused sample or sample components, for possible future reference, under conditions
suitable to maintain viability


3. Maintain a safe environment

Performance Criteria:

3.1. Use established safe work practices and personal protective equipment to ensure personal safety
and that of other laboratory personnel
3.2. Clean up spills using appropriate techniques to protect personnel, work area and environment
from contamination
3.3. Minimise the generation of wastes
3.4. Ensure the safe disposal of biohazardous materials and other laboratory wastes in accordance
with enterprise procedures


4. Maintain laboratory records

Performance Criteria:

4.1. Make entries on report forms or into computer systems, accurately calculating, recording or
transcribing required data as required
4.2. Update instrument maintenance logs as required by accreditation checklists
4.3. Maintain security and confidentiality of all clinical information, laboratory data and records

Learning Outcomes

Competency based

Details of Learning Activities

Practical classes

Online activities

Work sheets in class

Tutorials on work book questions

Teaching Schedule


Week beginningPracticalTheory-on line
9.2.15OHS review blood
Haem introduction
16.2.15 Films  Film Manual staining WCC automated 


Cells and documents

Stain theory



WBC manual

 Red cells and parameters. PCV.

Manual WCC

QC Protocols

Counting chambers

SOP requirements

2.3.15FBE parametersReviewCoulter

 Platelet count

manual and auto


FBE protocols


 Cell review

IM Test

NATA worksheet



23.3.15TEST WEEK 
30.3.15PTCoagulation Intro
13.4.15PT APTTTests
20.4.15PT APTTClotting Factors

Stago Speaker QC

Automated tests



Automated tests QC review

D Dimer Stago


QC review

Stago question sheets
18.5.15TutorialRevision terms 1and 2
25.5.15Finish any incomplete prac assessmentsWork book completion
1.6.15Test 35% 
8.6.15Additional week as required 
15.6.15Staff week 

Lab workflow, film protocols

Reporting protocols

FBE Normals

Normal film
13.7.15Intro to anaemia Fe deficiencyFe deficiency
20.7.15Megaloblastic anaemiaMegaloblastic anaemia
27.7.15NNANNA Deficiency testing
3.8.15Haemolytic anaemias 1 Reticulocytes



10.8.15Haemolysis 2Drugs, G6PD
17.8.15Haemolysis 3Metabolic
24.8.15Test week Review Questions
31.8.15Infectious diseasesBacterial and Viral infections



Malignancy of Blood Cells

Platelet disorders ITP, TTP


Spurious results

Platelets disorders, spurious results




Post splenectomy film






Prac assessment preliminary

12.10.15  Blood film assessment 


Prac assessment films -final

26.10.15Test (35%) 
2.11.15Extra testing if required 

Learning Resources

Prescribed Texts


Other Resources

Suggested texts for further information

Dacie and Lewis : Practical Haematology

Hoffbrand and Pettit : Haematology

Bain : Blood Cells a Practical Guide

Haematology Atlas (Sandoz)

Rozenberg: Microscopic Haematology

Howard and Hamilton: Haematology for images in haematology

RCPA website

ASH Bloodline website


Overview of Assessment

Assessment for this course may include tests, quizzes, assignments and practical assessment

Assessment Tasks

 two open book tests 35% each (70%)            Weeks of 1.6.2015 and 26.10.2015

6 online quizzes 5% each (30%)                      Weeks of  2.3.15

Practical assessments ongoing in all practical classes S/NYS
Practical work books- Results and questions to be completed during practical classes. S/ NYS                                                                                          

Assessment Matrix






Other Information

Assessment Information

This course is graded in accordance with competency-based assessment, but which also utilises graded assessment
CHD: Competent with High Distinction (80 – 100%)
CDI: Competent with Distinction (60 – 79%)
CC: Competent with Credit (50 – 59%)
CAG: Competency Achieved – Graded (0 – 49%)
NYC: Not Yet Competent
DNS: Did Not Submit for assessment


  • All assessment types must be passed (exams, prac, and assignments etc.). For example, if there are two tests you need to have an average of 50% to pass. You can’t make up marks from one type of assessment to another (e.g. pass the tests but fail the prac component).
  • Attendance at practical classes is compulsory. A minimum of 80% attendance is required to pass this course. If you are sick you should submit a medical certificate to 6th floor office addressed to the Program Coordinator
  • Late work that is submitted without an application for an extension will not be corrected.
  • APPLICATION FOR EXTENSION OF TIME FOR SUBMISSION OF ASSESSABLE WORK- A student may apply for an extension of up to 7 days from the original due date. They must lodge the application form (available online at least 24 hours before the due date. The application is lodged with the School Admin Office on Level 6, Bdg 51, or emailed to the Coordinator (
  • Students requiring extensions longer than 7 days must apply for Special Consideration (see the ‘Help me’ link in blackboard, via myRMIT studies or For missed assessments such as exams - you (& your doctor if you are sick) must fill out a special consideration form. This form must be lodged online with supporting evidence (e.g. medical certificate), prior to, or within, 48 hours of the scheduled time of examination.
  • If you miss an assessment task due to unavoidable circumstances, you need to follow the procedure of special consideration and apply within the allowed time frame.


Course Overview: Access Course Overview