Course Title: Perform microbiological tests

Part B: Course Detail

Teaching Period: Term1 2018

Course Code: BIOL5222C

Course Title: Perform microbiological tests

School: 174T School of VE Engineering, Health & Science

Campus: City Campus

Program: C5362 - Diploma of Laboratory Technology (Pathology Testing)

Course Contact: Namrita Kaul

Course Contact Phone: +61 3 9925 4837

Course Contact Email:

Name and Contact Details of All Other Relevant Staff

Joanne Bobbitt

Nominal Hours: 140

Regardless of the mode of delivery, represent a guide to the relative teaching time and student effort required to successfully achieve a particular competency/module. This may include not only scheduled classes or workplace visits but also the amount of effort required to undertake, evaluate and complete all assessment requirements, including any non-classroom activities.

Pre-requisites and Co-requisites


MSL973007 Perform microscopic examination

MSL973004 Perform aseptic techniques

Course Description

This unit of competency covers the ability to contribute to the culture, isolation and identification of microorganisms, such as bacteria, fungi, viruses, protozoans, algae and parasites, in order to investigate the physiology and pathology of plants and animals, monitor the natural environment, and to assist in the production of foods, pharmaceutical goods and other manufactured materials.

National Codes, Titles, Elements and Performance Criteria

National Element Code & Title:

MSL975001 Perform microbiological tests


1 Receive samples and process associated request forms

Performance Criteria:


Check samples and request form details before they are accepted


Return samples and request forms that do not comply with requirements to source with reasons for non-acceptance


Log samples, recording details that allow accurate tracking and chain of custody


Distribute samples for local testing or dispatch samples to other testing facilities


Store samples appropriately where testing or transport is to be delayed


2 Prepare for safe microbiological work and aseptic applications

Performance Criteria:


Select work area and equipment required for the safe handling of materials that may contain microorganisms of specified risk groups


Wear protective apparel, replacing it when contamination is suspected


Apply correct disinfection procedures to work areas before and after use


Locate relevant emergency equipment for timely response to microbiological accidents


Apply standard precautions when handling biological materials


Minimise the production and release of aerosols, using biological safety cabinets where necessary


Clean spills, and report all spills and suspected incidents to supervisor


Wash hands before and after laboratory work and when contamination is suspected


Ensure the safe disposal of biohazardous materials and other laboratory wastes in accordance with workplace procedures


3 Process samples for direct examination

Performance Criteria:


Prepare thin smears of samples for subsequent staining to enable microscopic identification of cells


Prepare liquid films of specimens for direct observation for motility or cell structure


Prepare samples to concentrate material for subsequent staining or microscopy


4 Prepare pure cultures for microbiological work and aseptic applications

Performance Criteria:


Select culture media to maximise growth of microorganisms and cells


Inoculate media aseptically, applying techniques suitable for purpose of culture


Incubate inoculated media in conditions to optimise growth of organisms and cells


Subculture on suitable media to optimise production of pure cultures


5 Perform procedures that can assist in the identification of microorganisms

Performance Criteria:


Select staining techniques to demonstrate required cellular characteristics


Stain prepared films to demonstrate diagnostically useful characteristics


Inoculate and incubate media with pure cultures to assist in the biochemical and immunological identification of microorganisms


Perform tests on pure cultures to assist in the biochemical and immunological identification of microorganisms


Perform antibiotic sensitivity testing, if required


6 Estimate the number and/or size of micro-organisms in samples

Performance Criteria:


Count cells in undiluted samples to indicate the dilution necessary to reliably count organisms in culture


Prepare serial dilutions of samples aseptically for culture and colony counting


Count colonies for calculating number of viable organisms per unit volume


Count microorganisms in samples and cultures using spectrometric and electronic methodologies, where relevant


Estimate and document uncertainty of measurement in accordance with workplace procedures, where relevant


7 Maintain records of laboratory work

Performance Criteria:


Make entries on report forms or into computer systems, accurately calculating, recording or transcribing data as required


Maintain instrument logs as required by accreditation checklists


Maintain security and confidentiality of all clinical information, laboratory data and records

Learning Outcomes

Details of Learning Activities

Practical laboratory exercises

Lectures on theoretical aspects of microbiology

Online research activities

Group discussion

You will be provided with revision questions for the material covered each week in class.

It is expected that for every hour of theory class, each student dedicates an hour of out-of-class study to the theoretical aspects of microbiology.

Teaching Schedule

WeekDate TopicPractical activitiesAssessment
15/2 Introduction to Microbiology No prac 
212/2 Microscopy: Smears & Staining; 

Safety, Laboratory Rules, Reports, Assessment, Aseptic Technique, Microscope set up

Prac1: Prokaryotic & Eukaryotic cells

Prac 2: Gram Stains

Prac 3: Gram, spore & negative stains. Plating

319/2 Bacterial Metabolism and growth requirements Prac 4: Effects of  varying incubation conditions on growthPrac report
426/2 Bacterial Growth & its measurement; Prac 5: Bacterial Population Counts 
55/3 Controlling Microbial Growth
Culture media
Prac 6: Sterilisation and disinfection 
612/3 Labour Day (no theory class)Prac 7: Preparation of HBA and MAC plates 
719/3Microbial geneticsPrac 8: Bacterial conjugation 
 1016/4Classification of organismsPrac 9: Basic Identification Tests 
1123/4  Introduction to IdentificationPrac 10:Biochemical Tests 1Prac report
1230/4 More Identification TestsPrac 11: Biochemical Tests 2
137/5 Gram Positive BacteriaPrac 12: Identification of Gram Positive Cocci 
1414/5 Gram Negative BacteriaPrac 13: Identification of Gram Negative Bacilli 
1521/5 REVISION Practical Exam 
Semester 2
12/7 Normal human floraPrac schedule to follow 
29/7 Immunology and epidemiology  
316/7 Skin and wound infections  
423/7 Antimicrobials and antivirals  
530/7 Virology  
66/8 Systemic and nervous system infections  
713/8 REVISION   
 27/8Mid Semester Break
93/9 Urinogenital tract infections  
1010/9 Respiratory tract infections  
 1117/9Gastrointestinal infections  
1224/9 Food microbiology  
131/10 Biotechnology applications  
148/10 Environmental microbiology  
1515/10 REVISIONPractical exam 
1729/10additional assessment  

Learning Resources

Prescribed Texts


Other Resources

On-line resources and other material will  be provided in class and will be available via Canvas

Recommended Text Book:

 Microbiology: An Introduction Global Edition                        

The Twelfth Edition of Tortora, Funke, and Case’s Microbiology: An Introduction focuses on big picture concepts and themes in microbiology, encouraging students to visualize and synthesize tough topics such as microbial metabolism, immunology, and microbial genetics. The text and accompanying resources also help students make connections between microbiology theory and disease diagnosis, treatment, and prevention.

Author: TORTORA Publisher: PEARSON EDUCATION AUSTRALIA Publication Date: 2015-07 Edition: 12

Overview of Assessment

written tests

practical reports

assessment of laboratory practical skills

laboratory logbook

Assessment Tasks

Formative assessment:
• Online Quizzes
• Problem sheets/work sheets.
• Feedback and self-assessment in laboratory/workplace

Students must pass all of the following assessment tasks to pass this course
4 x Theory Tests (2 per semester) @ 15 % each 60%
4 x Formal Practical Reports (2 per semester) @ 10 % each 40%
1 x Practical observation (1st semester) COMPETENCY satisfactory/not yet satisfactory 
1 x Practical examination (2nd semester) COMPETENCY satisfactory/not yet satisfactory
Log Book (throughout the year) COMPETENCY satisfactory/not yet satisfactory

Assessment Matrix

Other Information

Assessment information

This course is graded in accordance with competency-based assessment, but which also utilise graded assessment
CHD Competent with High Distinction (80 – 100%)
CDI Competent with Distinction (70 – 79%)
CC Competent with Credit (60 – 69%)
CAG Competency Achieved – Graded (50 – 59%)
NYC Not Yet Competent (0 – 49%)
DNS Did Not Submit for assessment

  • To pass the course you need to pass, on average, each type of assessment (exam, prac reports, and assignments etc.). For example, if there are two tests you need to have an average of 50% to pass. But you also need to have passed the other assessments as well. You can’t make up marks from one type of assessment to another (e.g. pass the tests but fail the prac reports).
  • Extension of time for submission of assessable work- A student may apply for an extension of up to 7 days from the original due date. They must lodge the application form (available on the web ) at least the day before the due date. The application form is emailed to the Program Coordinator ( Students requiring longer extensions must apply for Special Consideration (form available on the Web). For missed assessments such as exams- you (& your doctor if you are sick) must fill out a special consideration form. This form must be lodged at the HUB or online with supporting evidence (eg medical certificate), prior to, or within, 48 hours of the scheduled time of examination.
  • Late work that is submitted without an application for an extension will not be corrected
  • If you miss an assessment task due to unavoidable circumstances, you need to follow the procedure of special consideration and apply within the allowed time frame.
  • Students must attend at least 80% of practical classes. If you miss a prac class you should provide a copy of your medical certificate or other evidence (court appearance, funeral notice, statutory declaration) to the teacher.
  • Plagiarism- Plagiarism is the presentation of the work, idea or creation of another person as though it is your own. It is a form of cheating and is a very serious academic offence that may lead to expulsion from the University. Plagiarised material can be drawn from, and presented in, written, graphic and visual form, including electronic data and oral presentation. Plagiarism occurs when the origin of the material used is not appropriately cited.
    It also occurs through enabling plagiarism, which is the act of assisting or allowing another person to plagiarise or to copy your own work. Please make sure you consider this carefully in completing all your work and assessments in this course and if you are unsure about whether you might have plagiarised, seek help from your teacher.

Course Overview: Access Course Overview