Course Title: Prepare, standardise and use solutions

Part B: Course Detail

Teaching Period: Term2 2017

Course Code: BIOL5229C

Course Title: Prepare, standardise and use solutions

School: 174T School of VE Engineering, Health & Science

Campus: City Campus

Program: C5363 - Diploma of Laboratory Technology (Biotechnology)

Course Contact: Amber Mitton

Course Contact Phone: +61 3 9925 8053

Course Contact Email:

Name and Contact Details of All Other Relevant Staff

Rauha Quazi

+61 3 9925 4277

Nominal Hours: 100

Regardless of the mode of delivery, represent a guide to the relative teaching time and student effort required to successfully achieve a particular competency/module. This may include not only scheduled classes or workplace visits but also the amount of effort required to undertake, evaluate and complete all assessment requirements, including any non-classroom activities.

Pre-requisites and Co-requisites


Course Description

This unit of competency covers the ability to prepare, standardise and monitor the quality of solutions.

National Codes, Titles, Elements and Performance Criteria

National Element Code & Title:

MSL974001 Prepare, standardise and use solutions


1 Prepare solutions

Performance Criteria:

1.1 Select appropriate proce dure for solution preparation 1.2 Select equipment, materials and solvent of specified purity 1.3 Measure appropriate quantities of reagents for solution preparation and record data. 1.4 Select and assemble specified laboratory equipment and appropriate grade of glassware1.5 Perform specified dilutions 1.6 Prepare solutions to achieve homogeneous mix of the specified concentration 1.7 Label and store solutions to maintain identity and stability


2 Standardise and use volumetric solutions

Performance Criteria:

2.1 Assemble appropriate laboratory equipment 2.2 Perform serial dilutions as required 2.3 Standardise the solution to the required specified range and precision 2.4 Label and store solutions to maintain identity and stability 2.5 Use standard volumetric solutions to determine concentration of unknown solutions


3 Calculate and record data

Performance Criteria:

3.1 Calculate specified concentrations 3.2 Use authorised procedure if data is to be modified 3.3 Estimate and document uncertainty of measurement in accordance with workplace procedures, as required 3.4 Record all relevant details according to laboratory procedures and report results 3.5 Report concentration with appropriate units


4 Monitor the quality of laboratory solutions

Performance Criteria:

4.1 Check solutions for visual deterioration and expiry date 4.2 Re-standardise or dispose of dated or deteriorated solutions 4.3 Record details and label solutions according to laboratory procedures


5 Maintain a safe work environment

Performance Criteria:

5.1 Use established safe work practices and personal protective equipment (PPE) to ensure personal safety and that of other laboratory personnel 5.2 Clean up spills using appropriate techniques to protect personnel, work area and environment 5.3 Minimise generation of waste and environmental impacts 5.4 Ensure the safe collection of laboratory and hazardous waste for subsequent disposal 5.5 Store equipment and reagents as required

Learning Outcomes

Details of Learning Activities

  1. Lectures
  2. Online learning activities
  3. Practicals

Teaching Schedule


Week beginning







1.1 Introduction to acids & bases, pH. Measuring pH

Lab 1
(i) Prepare an anhydrous KHP

(ii) Measuring pH using Indicators


Log Book & Lab Skills



1.2: Strong & weak acids & bases, pKa, Titration,
Intro to standardisation Titrating accurately and precisely – Standard & Random errors

Lab 2

Analysis of Vinegar by Titration


Log Book & Lab Skills




1.3 Primary Standards and Standardisation of NaOH

Lab 3

Standardising a NaOH solution

Log Book & Lab Skills

Standardisation 1




1.4 Introduction to Buffers, Predicting pH of buffer solutions

Lab 4
Measuring pH using the pH meter

Log Book & Lab Skills

Online Quiz 1



1.5 Buffering capacity, pH compared to pKa, buffer concentration,
Titration Curves

Lab 5
Titration curve of a strong base against a weak acid

Log Book & Lab Skills

Lab Report 1
(done in class)



1.6 Predicting buffer pH

Lab 6

Buffers II: Predicting buffer pH

Log Book & Lab Skills

Online Quiz 2



Lab only

Last chance Assessment catch up session*
Lab 3 Standardising a NaOH solution

 Lab 5 Titration curve
NOTE: There is not enough time to catch up both assessments

Catch up: Lab 3 OR 5

Submit logbook & checklist for mid-semester review





Mid Semester exams

 No classes



 28 Aug

28 August – 1 Sept: Mid semester Break





2.1 Complex ions and Complexometric titrations  (lab only)


Lab 7

Testing hardness of water by a complexometric titration

Log Book & Lab Skills

Standardisation 2

Lab Report 2

(done in class)



2.2 Oxidation & Reduction (Redox) Reactions

Last chance Assessment catch up session*
Lab 7 

Catch up: Lab 7

Online Quiz 3



2.3 (lab only)

Lab 8 

Redox titration:

(i) Standardisation of KI with ascorbic acid

(ii) Iodometry of vitamin C

Log Book & Lab Skills

Standardisation 3




Public holiday Fri 29 Sept – no classes



2.4 Serial dilutions – refresher

Last chance Assessment catch up session*
Lab 8 

Catch up: Lab 8



2.5 (Lab only)

Lab 9

Serial dilutions

Log Book & Lab Skills

Online Quiz 4




Last chance Assessment catch up session*
Lab 9

Catch up: Lab 9

Submit logbook & checklist for final-semester review






Theory Exam



Alternative assessment (special consideration only)


Learning Resources

Prescribed Texts


Other Resources

Handouts and Online Learning materials will be provided during the course.

A bound (non-spiral) exercise book is needed to be used as a logbook.

A scientific calculator is needed to perform calculations in class and during exam.

Safety equipment: lab coat, closed shoes, gloves

Recommended textbook

 Bettelheim FA, Brown WH, Campbell MK, Farrell SO (2013) General, Organic and Biochemistry  10th Ed. Thomson/Brooks-Cole, Australia

Overview of Assessment

written tests

assessment of laboratory skills

practical reports

Assessment Tasks




Assessment 1. Laboratory practice

a.    Logbook Records
Students must attend and record at least 80% of the labs.


Complete logbook for all attended lab sessions.

Submit logbook & checklist by Friday 5 pm of week 7 (mid semester review) and week 15 (final assessment)


b.    Labskills

Lab skills will be assessed each week along with the logbook records using the ‘logbook & labskills checklist’.

To pass the course students MUST
attend 80% of labs AND:

1.    Standardisethree (3) different solutions to the required precision and accuracy

2.    Do a titration using each of the three standardised solution to the required precision and accuracy

3.    Create a titration curve and
use it to identify the equivalence point of a titration to the required precision and accuracy

 Weeks 3, 5, 9, 11, 14


Satisfactory at all tasks

c.    2 Lab Reports (to be submitted via Turnitin)


The experiments are completed in
(1) the week 5 class and (2) the week 9 class

= 20%

Assessment 2. Theory Exam

Week 16


Assessment 3. Online quizzes

4 Online quizzes 5% each

Week 4, 6, 10, 14


Assessment Matrix

Other Information

Assessment Information

This course is graded in accordance with competency-based assessment, but which also utilise graded assessment
CHD Competent with High Distinction (80 – 100%)
CDI Competent with Distinction (70 – 79%)
CC Competent with Credit (60 – 69%)
CAG Competency Achieved – Graded (50 – 59%)
NYC Not Yet Competent
DNS Did Not Submit for assessment

  • To pass the course you need to pass, on average, each type of assessment (exams, pracs, and assignments etc.) For example, if there are two exams you need to have an average of 50% to pass and you also need to have passed the other assessment types. You can’t make up marks from one type of assessment to another (e.g. pass the exams but fail the prac component).
  • Late work that is submitted without an application for an extension (see below) will not be corrected.
  • APPLICATION FOR EXTENSION OF TIME FOR SUBMISSION OF ASSESSABLE WORK - A student may apply for an extension of up to 7 days from the original due date. They must lodge the application form (available online at least 24 hours before the due date. The application is lodged with the School Admin Office on Level 6, Bdg 51. Students requiring longer extensions must apply for SPECIAL CONSIDERATION.
  • For missed assessments such as exams- you (& your doctor if you are sick) must fill out a special consideration form. This form must be lodged online with supporting evidence prior to, or within, 48 hours of the scheduled time of examination
  • A minimum of 80% attendance at practical classes is required to pass this course. If you are sick you should submit a medical certificate to 6th floor office addressed to the Program Coordinator. If you miss a practical class that is a prac report write up you need to provide a medical certificate (or other evidence) and arrange an alternative report with your teacher.

Plagiarism is the presentation of the work, idea or creation of another person as though it is your own. It is a form of cheating and is a very serious academic offence that may lead to expulsion from the University. Plagiarised material can be drawn from, and presented in, written, graphic and visual form, including electronic data and oral presentation. Plagiarism occurs when the origin of the material used is not appropriately cited. It also occurs through enabling plagiarism, which is the act of assisting or allowing another person to plagiarise or to copy your own work. Please make sure you consider this carefully in completing all your work and assessments in this course and if you are unsure about whether you might have plagiarised, seek help from your teacher. 

Course Overview: Access Course Overview